MAM: a “new” high‐incidence antigen found on multiple cell lines

Montgomery, William M.; Nance, Sandra; Donnelly, Sarah F.; Brady, Tim; Anderson, Garth; Mintz, Paul D.; Moulds, Marilyn; Daniels, Geoff; Spring, Frances A.; Molina, Nelly Stella Roa; Asis, Eleanor A. De; Olivares, Esperanza

doi:10.1046/j.1537-2995.2000.40091132.x

Cited by 11 publications

(6 citation statements)

References 17 publications

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“…According to the International Society of Blood Transfusion (ISBT) in human medicine, the criteria for inclusion as a high‐incidence antigen are as follows: (1) an incidence of >90% in most populations tested, but usually >99%; (2) distinction from all other high‐incidence specificities; and (3) demonstration that the antigen is lacking in at least 2 siblings, giving evidence that the negative phenotype is genetically determined . Studies identifying high‐incidence antigens usually report only a few individuals lacking the antigen . In this study, the prevalence of the Dal − minor allele was >10% in several breeds and thus the Dal antigen seems to be a true blood group system rather than a high‐incidence antigen.…”

Section: Discussionmentioning

confidence: 96%

Prevalence and Mode of Inheritance of theDalBlood Group in Dogs in North America

Goulet

Giger

Arsenault

et al. 2017

Veterinary Internal Medicne

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BackgroundThe Dal blood group system was identified a decade ago by the accidental sensitization of a Dal− Dalmatian with a Dal+ blood transfusion. Similar Dal‐related blood incompatibilities have been suspected in other Dalmatians, Doberman Pinschers, and other breeds.ObjectivesTo determine the prevalence and mode of inheritance of the Dal antigen expression in dogs.AnimalsA total of 1130 dogs including 128 Dalmatians, 432 Doberman Pinschers, 21 Shih Tzus, and 549 dogs of other breeds including 228 blood donors were recruited from North America between 2008 and 2015.MethodsProspectively, dogs were blood typed for Dal applying a gel column technique using polyclonal canine anti‐Dal sera. Pedigrees from 8 typed families were analyzed.ResultsThe prevalence of the Dal+ blood type varied between 85.6 and 100% in Dalmatians and 43.3–78.6% in Doberman Pinschers depending on geographical area. Dal− dogs were identified mostly in Dalmatians (15/128; 11.7%), Doberman Pinschers (183/432; 42.4%), and Shih Tzus (12/21; 57.1%), and sporadically in mixed‐breed dogs (3/122; 2.5%), Lhasa Apsos (1/6) and Bichon Frises (1/3). Only 6/245 (2.4%) blood donors were found to be Dal−, including 5 Doberman Pinschers. The mode of inheritance of the Dal+ phenotype was determined to be autosomal dominant.Conclusions and Clinical ImportanceThe high percentage of Dal− Doberman Pinchers, Dalmatians and Shih Tzus increases their risk of being sensitized by a blood transfusion from the common Dal+ donor. Extended Dal typing is recommended in those breeds and in dogs when blood incompatibility problems arise after initial transfusions.

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Section: Discussionmentioning

confidence: 96%

Prevalence and Mode of Inheritance of theDalBlood Group in Dogs in North America

Goulet

Giger

Arsenault

et al. 2017

Veterinary Internal Medicne

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“…There are currently nine antigens in the 901 series (Table 8). Since 1995, two new antigens, both of high incidence, have been added: 901015 (ABTI) [63] and 901016 (MAM) [66], although 901015 (ABTI) has now become VEL2 of the 211 collection. Three antigens became the sole antigens of new blood group systems and their 901 numbers are obsolete: 901006 (Ok a ) has become OK1; 901007 (JMH) has become JMH1; and 901011 (MER2) has become RAPH1 (Table 1).…”

Section: Series High‐incidence Antigensmentioning

confidence: 99%

Blood group terminology 2004: from the International Society of Blood Transfusion committee on terminology for red cell surface antigens

Fletcher²,

et al. 2004

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“…4 ), including a wide variety of haematopoietic tissues and cell types 16 . Conflicting data regarding MAM expression on platelets 17 , coupled with reports of foetal and newborn low platelet counts in infants of MAM-negative individuals (P1, P2, P9; data not shown) prompted us to investigate MAM expression on platelets. We found that MAM is expressed on platelets and MAM expression is independent of the activation status of platelets (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

Disruption of the tumour-associated EMP3 enhances erythroid proliferation and causes the MAM-negative phenotype

et al. 2020

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The clinically important MAM blood group antigen is present on haematopoietic cells of all humans except rare MAM-negative individuals. Its molecular basis is unknown. By whole-exome sequencing we identify EMP3 , encoding epithelial membrane protein 3 (EMP3), as a candidate gene, then demonstrate inactivating mutations in ten known MAM-negative individuals. We show that EMP3, a purported tumour suppressor in various solid tumours, is expressed in erythroid cells. Disruption of EMP3 by CRISPR/Cas9 gene editing in an immortalised human erythroid cell line (BEL-A2) abolishes MAM expression. We find EMP3 to associate with, and stabilise, CD44 in the plasma membrane. Furthermore, cultured erythroid progenitor cells from MAM-negative individuals show markedly increased proliferation and higher reticulocyte yields, suggesting an important regulatory role for EMP3 in erythropoiesis and control of cell production. Our data establish MAM as a new blood group system and demonstrate an interaction of EMP3 with the cell surface signalling molecule CD44.

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MAM: a “new” high‐incidence antigen found on multiple cell lines

Cited by 11 publications

References 17 publications

Prevalence and Mode of Inheritance of theDalBlood Group in Dogs in North America

Prevalence and Mode of Inheritance of theDalBlood Group in Dogs in North America

Blood group terminology 2004: from the International Society of Blood Transfusion committee on terminology for red cell surface antigens

Disruption of the tumour-associated EMP3 enhances erythroid proliferation and causes the MAM-negative phenotype

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