2006
DOI: 10.1002/dvdy.20915
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Mammalian nuclear transfer

Abstract: During development, the genetic content of each cell remains, with a few exceptions, identical to that of the zygote. Differentiated cells, therefore, retain all the genetic information necessary to generate an entire organism (nuclear totipotency). Nuclear transfer (NT) was initially developed to test experimentally this concept by cloning animals from differentiated cells. It has, since then, been used to study the role of genetic and epigenetic alterations during development and disease. In this review, we … Show more

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Cited by 112 publications
(91 citation statements)
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“…102 Since the first success of cloning in sheep, 108 viable offspring have been obtained by SCNT in ungulates (cattle, 32 goat, 4 mule, 115 horse, 33 pig, 84 and mouflon 65 ), rodents (mouse 104 and rat 112 ), lagomorphs (rabbit 13 ), and carnivores (cat 92 and dog 59 ). Such cloning has many potential benefits and research applications, including study of genetic and epigenetic mechanisms underlying developmental biology, aging, and carcinogenesis 67 ; generation of organs for xenotransplantation; development of transgenic animals for production of valuable recombinant proteins; and preservation of endangered species. 103 The major impediment to the practical use of SCNT technology is that less than 5% of SCNT embryos develop normally to term.…”
Section: Introductionmentioning
confidence: 99%
“…102 Since the first success of cloning in sheep, 108 viable offspring have been obtained by SCNT in ungulates (cattle, 32 goat, 4 mule, 115 horse, 33 pig, 84 and mouflon 65 ), rodents (mouse 104 and rat 112 ), lagomorphs (rabbit 13 ), and carnivores (cat 92 and dog 59 ). Such cloning has many potential benefits and research applications, including study of genetic and epigenetic mechanisms underlying developmental biology, aging, and carcinogenesis 67 ; generation of organs for xenotransplantation; development of transgenic animals for production of valuable recombinant proteins; and preservation of endangered species. 103 The major impediment to the practical use of SCNT technology is that less than 5% of SCNT embryos develop normally to term.…”
Section: Introductionmentioning
confidence: 99%
“…It was discovered first in Xenopus and later in many other animal species that once transfered into enucleated eggs, differentiated somatic cells could be reprogrammed to a totipotent status and to generate live animals [1]. The mechanism underlying the reprogramming process has been the focus of intensive research.…”
Section: Introductionmentioning
confidence: 99%
“…The same experiment was repeated 30 years later using the sheep as a model [2]. However, the initial emphasis that has welcomed the production of Dolly the sheep has been progressively quenched in the light of the multitude of developmental defects described in clones [3]. In particular, an emerging insight is that development and function of extra-embryonic membranes is particularly affected by somatic cell nuclear transfer (SCNT) [4,5].…”
Section: Introductionmentioning
confidence: 99%
“…Global changes in DNA methylation and modifications of histone proteins are the molecular fingerprint of the physiological programming taking place during normal development, and a great deal of effort is currently being put toward establishing how these global changes are preserved or altered in cloned embryos [3].…”
Section: Introductionmentioning
confidence: 99%