Management of Renin-Angiotensin-Aldosterone System Blockade in Kidney Transplant Recipients

Heleniak, Zbigniew; Kuźmiuk-Glembin, Izabella; Adrych, Dorota; Garnier, Hanna; Wiśniewski, Jakub Bożydar; Rutkowski, Przemysław; Rutkowski, Bolesław; Tylicki, Leszek; Dębska-Ślizień, Alicja

doi:10.1016/j.transproceed.2018.03.027

Cited by 4 publications

(1 citation statement)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, many studies [94][95][96][97] have confirmed that the RAS system is closely related to proteinuria caused by various diseases, such as diabetic nephropathy and CKD. CKD is associated with hypoxia which can activate HIF-1 [98] and induce profibrogenic changes in proximal tubular epithelial cells and interstitial fibroblasts, leading to further aggravation of proteinuria [99].…”

Section: Hif-1 Ras Vegf and Age-rage Signaling Pathwaymentioning

confidence: 99%

Identifying Synergistic Mechanisms of Multiple Ingredients in Shuangbai Tablets against Proteinuria by Virtual Screening and a Network Pharmacology Approach

Hao

et al. 2020

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Shuangbai Tablets (SBT), a traditional herbal mixture, has shown substantial clinical efficacy. However, a systematic mechanism of its active ingredients and pharmacological mechanisms of action against proteinuria continues being lacking. A network pharmacology approach was effectual in discovering the relationship of multiple ingredients and targets of the herbal mixture. This study aimed to identify key targets, major active ingredients, and pathways of SBT against proteinuria by network pharmacology approach combined with thin layer chromatography (TLC). Human phenotype (HP) disease analysis, gene ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, and molecular docking were used in this study. To this end, a total of 48 candidate targets of 118 active ingredients of SBT were identified. Network analysis showed PTGS2, ESR1, and NOS2 to be the three key targets, and beta-sitosterol, quercetin, and berberine were the three major active ingredients; among them one of the major active ingredients, quercetin, was discriminated by TLC. These results of the functional enrichment analysis indicated that the most relevant disease including these 48 candidate proteins is proteinuria, SBT treated proteinuria by sympathetically regulating multiple biological pathways, such as the HIF-1, RAS, AGE-RAGE, and VEGF signaling pathways. Additionally, molecular docking validation suggested that major active ingredients of SBT were capable of binding to HIF-1A and VEGFA of the main pathways. Consequently, key targets, major active ingredients, and pathways based on data analysis of SBT against proteinuria were systematically identified confirming its utility and providing a new drug against proteinuria.

show abstract