Water-energy issues are of growing importance in the context of water shortages, higher energy, material costs, and climatic change.Wastewater from industry and domestic use should meet a specific set of requirements before being returned to the natural environment (Abou-Taleb et al., 2020). In all countries, regardless of the income levels, appropriate management of these wastewaters is a costly and persistent challenge (Wallace et al., 2015). To meet this challenge, it is important to find alternative solutions for wastewater treatment using innovative and developed technologies (Abou-Elela et al., 2019b). New technologies typically follow a development process that leads from laboratory and bench scale investigations to pilot studies and to initiate use or "full-scale demonstrations" as well (Abou-Elela et al., 2018). Biofilm-based passive aeration systems are one of the wastewater treatment systems that have attracted recent attention as an alternative energy efficient and low maintenance technologies for the treatment of municipal wastewater (Clifford