Manganese effects on haematopoietic cells and circulating coelomocytes of Asterias rubens (Linnaeus)

Oweson, Carolina; Sköld, Helén Nilsson; Pinsino, Annalisa; Matranga, Valeria; Hernroth, Bodil

doi:10.1016/j.aquatox.2008.05.016

Cited by 47 publications

(25 citation statements)

References 32 publications

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“…As previously reported, the accumulation of manganese into the coelomic fluid of exposed sea stars (Asterias rubens) induces the proliferation of haematopoietic cells (Oweson et al, 2008). Specifically, by using the substitute nucleotide 5-bromo-2'-deoxyuridine (BrdU) for tracing cell division, and by recording the mitotic index after nuclei staining, authors found that manganese induced the proliferation of cells from a putative haematopoietic tissue, the coelomic epithelium.…”

Section: How Does Manganese Affect Echinoderm Immune Cells?mentioning

confidence: 64%

“…In addition, manganese alters the immune system of sponges (Geodia cydonium, Crella elegans and Chondrosia reniformis) by inhibiting the activity of the 2', 5'-oligoadenylate synthetase (2-5A synthetase), an enzyme known to be involved in the functioning of the immune system of vertebrates (Saby et al, 2009). Surprisingly, in contrast to what was recorded in crustaceans and molluscs, in echinoderms (Asterias rubens) manganese exposure stimulated haematopoiesis, thus causing an increase in the number of circulating immune cells (Oweson et al, 2008). Manganese effects on Asterias rubens immune system will be discussed in detail in the next sections.…”

Section: Manganese Toxicity In Marine Invertebratesmentioning

confidence: 75%

“…Manganese accumulation in the sea star Asterias rubens has shown linearity with time up to 23 days at low concentrations (0.1 mg l -1 ), but its saturation kinetics were very fast at higher concentrations (Hansen & Bjerregaard, 1995). In fact, it was found that steady-state levels were reached in the coelomic fluids after only 5 days of exposure to 5.5 mg l -1 (Oweson et al, 2008).…”

Section: Manganese Toxicity In Marine Invertebratesmentioning

confidence: 95%

See 2 more Smart Citations

Manganese: A New Emerging Contaminant in the Environment

Pinsino¹,

Matranga²,

Roccheri³

2012

Environmental Contamination

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Section: How Does Manganese Affect Echinoderm Immune Cells?mentioning

confidence: 64%

Section: Manganese Toxicity In Marine Invertebratesmentioning

confidence: 75%

Section: Manganese Toxicity In Marine Invertebratesmentioning

confidence: 95%

See 1 more Smart Citation

Manganese: A New Emerging Contaminant in the Environment

Pinsino¹,

Matranga²,

Roccheri³

2012

Environmental Contamination

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“…Earlier investigations of the immune response in A. rubens have shown that although there was an increase in the amount of coelomocytes, the coelomocytes themselves showed no upregulated proliferation (Holm et al, 2008;Oweson et al, 2008). When investigating other areas of the sea star, however, it was found that the coelomic epithelium, axial organ (Leclerc et al,'87), and Tiedemann body all responded with increased cell proliferation (Holm et al, 2008;Oweson et al, 2008).…”

mentioning

confidence: 91%

Possibility of mixed progenitor cells in sea star arm regeneration

et al. 2010

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In contrast to most vertebrates, invertebrate deuterostome echinoderms, such as the sea star Asterias rubens, undergo regeneration of lost body parts. The current hypothesis suggests that differentiated cells are the main source for regenerating arm in sea stars, but there is little information regarding the origin and identity of these cells. Here, we show that several organs distant to the regenerating arm responded by proliferation, most significantly in the coelomic epithelium and larger cells of the pyloric caeca. Analyzing markers for proliferating cells and parameters indicating cell ageing, such as levels of DNA damage, pigment, and lipofuscin contents as well as telomere length and telomerase activity, we suggest that cells contributing to the new arm likely originate from progenitors rather than differentiated cells. This is the first study showing that cells of mixed origin may be recruited from more distant sources of stem/progenitor cells in a sea star, and the first described indication of a role for pyloric caeca in arm regeneration. Data on growth rate during arm regeneration further indicate that regeneration is at the expense of whole animal growth. We propose a new working hypothesis for arm regeneration in sea stars involving four phases: wound healing by coelomocytes, migration of distant progenitor cells of mixed origin including from pyloric caeca, proliferation in these organs to compensate for cell loss, and finally, local proliferation in the regenerating arm.

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“…metallurgic/chemical/agrochemical products, municipal wastewater discharges, etc) received the attention of the scientific world that considered Mn as a potential emerging contaminant in the environment, especially in the marine milieu (CICAD 63, 2004;Pinsino et al, 2012). In marine organisms some studies showed that, an Mn overload causes toxicity, although the cause-effect evidence has not been well elucidated (Regoli et al, 1991;Krång and Rosenqvist, 2006;Oweson et al, 2008;Oweson and Hernroth, 2009). Recently, we took advantage of the amenable model, the Mediterranean sea urchin Paracentrotus lividus, to investigate the potential toxicity of Mn on embryonic development, using biological and biochemical approaches.…”

Section: Introductionmentioning

confidence: 99%

Manganese overload affects p38 MAPK phosphorylation and metalloproteinase activity during sea urchin embryonic development

Pinsino

Roccheri

Matranga

2014

Marine Environmental Research

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a b s t r a c tIn the marine environment, manganese represents a potential emerging contaminant, resulting from an increased production of manganese-containing compounds. In earlier reports we found that the exposure of Paracentrotus lividus sea urchin embryos to manganese produced phenotypes with no skeleton. In addition, manganese interfered with calcium uptake, perturbed extracellular signal-regulated kinase (ERK) signaling, affected the expression of skeletogenic genes, and caused an increase of the hsc70 and hsc60 protein levels. Here, we extended our studies focusing on the temporal activation of the p38 mitogen-activated protein kinase (p38 MAPK) and the proteolytic activity of metalloproteinases (MMPs). We found that manganese affects the stage-dependent dynamics of p38 MAPK activation and inhibits the total gelatin-auto-cleaving activity of MMPs, with the exclusion of the 90e85 kDa and 68e58 kDa MMPs, whose levels remain high all throughout development. Our findings correlate, for the first time to our knowledge, an altered activation pattern of the p38 MAPK with an aberrant MMP proteolytic activity in the sea urchin embryo.

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Manganese effects on haematopoietic cells and circulating coelomocytes of Asterias rubens (Linnaeus)

Cited by 47 publications

References 32 publications

Manganese: A New Emerging Contaminant in the Environment

Manganese: A New Emerging Contaminant in the Environment

Possibility of mixed progenitor cells in sea star arm regeneration

Manganese overload affects p38 MAPK phosphorylation and metalloproteinase activity during sea urchin embryonic development

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