Manganese(II) chloride alters behavioral and neurochemical parameters in larvae and adult zebrafish

Altenhofen, Stefani; Wiprich, Melissa Talita; Nery, Laura Roesler; Leite, Carlos Eduardo; Vianna, Mônica Ryff Moreira Roca; Bonan, Carla Denise

doi:10.1016/j.aquatox.2016.11.013

Cited by 74 publications

(44 citation statements)

References 57 publications

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“…We placed animals individually in experimental tanks (30 cm long × 15 cm high × 10 cm wide) with water and recorded them for 6 min. After 60 s of habituation, we analyzed the locomotion and exploratory patterns of the fish using EthoVision XT tracking software (version 11.5, Noldus, Wageningen, The Netherlands) at a rate of 30 positions per second (Altenhofen et al, 2017b). We evaluated the following behavioral parameters: distance (m), mean speed (m/s), time spent in the upper zone (bottom vs. upper levels; s), crossings, and latency to the upper zone (s).…”

Section: Novel Tank Testmentioning

confidence: 99%

“…We evaluated inhibitory avoidance using a glass tank (18 cm long × 9 cm wide × 7 cm high) with two equal-size compartments, designated hereon as dark and white and divided by a sliding guillotine-type partition (9 cm × 7 cm; Blank et al, 2009;Altenhofen et al, 2017b;Bridi et al, 2017). We defined compartments with opaque plastic self-adhesive films in black or white colors that externally covering the walls, the floor, and the corresponding sides of the partition.…”

Section: Aversive Memorymentioning

confidence: 99%

“…The zebrafish (Danio rerio) is a highly reliable model. It usually takes zebrafish less than 2 h to learn a task (Aoki et al, 2015), and the retention time can exceed 24 h (Altenhofen et al, 2017b;Bridi et al, 2017). Despite the increasing number of toxicological studies about larvicides and insecticides with an indication for continuous use, there are few studies about the effects of pyriproxyfen in fish, which is an important model for pollution studies (Charreton et al, 2015;Kais et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Pyriproxyfen Exposure Impairs Cognitive Parameters and Alters Cortisol Levels in Zebrafish

Gusso

Reolon

Gonzalez

et al. 2020

Front. Behav. Neurosci.

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Pyriproxyfen is one of the most used larvicides and insecticides; it acts as an analog of juvenile insect hormone (a growth regulator). It is highly toxic during all stages of mosquito development, suppresses metamorphosis, and interferes in insect reproduction and proliferation. Pyriproxyfen and its main metabolite have been shown to affect brain development in rodents. This compound is employed mainly to eliminate outbreaks of the genus Aedes, even in potable water. Despite the increasing number of toxicological studies about larvicides and insecticides-with an indication of continuous use-there have been few studies about the effects of pyriproxyfen in non-target species such as fish. This study evaluated the effects of pyriproxyfen on behavioral, cognitive, and endocrine parameters in zebrafish. We exposed adult zebrafish to different pyriproxyfen (Pestanal) concentrations (0.125, 0.675, and 1.75 mg/l) for 96 h. We analyzed behavioral parameters, memory, cortisol levels, and gene expression of glucocorticoid receptor (gr) and corticotrophin-releasing factor (crf) after pyriproxyfen exposure. This exposure did not alter locomotion (distance or mean speed), anxiety-like behavior (latency to enter to the top zone of the tank or time in the top zone of the tank), and social or aggressive behavior. However, there was impaired inhibitory avoidance memory at all tested pyriproxyfen concentrations. Cortisol levels were reduced in exposed groups when compared to control or vehicle. However, gr and crf gene expression in pyriproxyfen-treated animals were unaltered when compared to control or vehicle groups. Taken together, these findings indicate that pyriproxyfen may induce cognitive impairment and altered cortisol levels in zebrafish, a non-target species.

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Section: Novel Tank Testmentioning

confidence: 99%

Section: Aversive Memorymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Pyriproxyfen Exposure Impairs Cognitive Parameters and Alters Cortisol Levels in Zebrafish

Gusso

Reolon

Gonzalez

et al. 2020

Front. Behav. Neurosci.

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“…Manganese, an example of these substances, is a natural element, essential for diverse physiologic processes, including metabolism of amino acids, lipids, proteins and carbohydrates (ERIKSON et al, 2005), immunologic system, regulation of cell energy, growth of the bone and connective tissue, blood clotting (ERIKSON and ASCHNER, 2003), and for some enzymes related to the synthesis and metabolism of neurotransmitters (GOLUB et al, 2005). However, at high concentrations or prolonged exposure periods, it shows toxicity for different organs in a wide spectrum of animals (ZHANG et al, 2003;ALTENHOFEN et al, 2017;CARVALHO et al, 2018). Its occurrence in aquatic environments may be caused by anthropogenic activities as well, especially in regions near to the industries responsible for the manufacture of iron and steel, use of fertilizers, fungicides and animal supplementation (PATIL et al, 2016).…”

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confidence: 99%

Evaluation of intestinal histological damage in zebrafish exposed to environmentally relevant concentrations of manganese

Rodrigues

Souza

Silva

et al. 2018

CeN

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Manganese is an essential element for many physiological processes. However, in prolonged exposures or at high concentrations it may present toxicity to several organs, which has made it an object of study in pharmacological, behavioral, neurological and environmental research in different organisms. Thus, it was aimed to evaluate the damage caused by acute and chronic exposures to this element, through the histopathological analysis of the zebrafish (Danio rerio) intestine. Samples were embedded in paraffin for further processing in rotating microtome, and the slides were stained with Alcian Blue and hematoxylin for observation and analysis under light microscopy. The results demonstrated the potential of the metal to cause histological changes in this organ, such as fusion of villi (in 0.5 and 4.0 mg L-1), and to increase by more than 50% the number of goblet cells (in 8.0 and 16 mg L-1) in the region analyzed, mainly in the highest concentrations of Manganese Chloride and in chronic exposures, characterizing a possible inflammation of the intestinal epithelium. Thus, the histological analysis of intestines demonstrated to be a reliable alternative for the toxicological evaluation. It is important to monitor the concentrations of this and other metals in the water bodies, given their toxicity.

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“…Behavioral analysis can be considered as a robust and sensitive tool for detecting sub-lethal effects of chemical on fish (Kane et al 2004;Eissa et al 2010). Indeed, numerous neurotoxic chemicals, such as pesticides and metals, may provoke abnormal swimming behavior or compromise swimming ability in fish and other aquatic organisms (Altenhofen et al 2017;Qiang et al 2016). In this work, effects of 2,4-D sublethal concentrations on the swimming behavior of larvae were assessed by measuring thigmotaxis behavior and the lightdark transition response at 120 hpf.…”

Section: Discussionmentioning

confidence: 99%

2,4-Dichlorophenoxyacetic acid herbicide effects on zebrafish larvae: development, neurotransmission and behavior as sensitive endpoints

Gaaied

Oliveira

Domingues

et al. 2019

Environ Sci Pollut Res

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Assessment of pesticides toxicity using zebrafish early life stages is relevant for aquatic systems safety. This study aimed to evaluate the short-term effects of 2,4-dichlorophenoxyacetic acid (2,4-D) on zebrafish (Danio rerio) embryos from 3 h post fertilization to 96 hpf. A set of 2,4-D concentrations ranging from 0.32 to 80 mg/L were tested and median lethal concentration (LC 50) at 96-h was calculated as 2.86 mg/L. A sub-teratogenic concentrations range from 0.02 to 0.8 mg/L was then used to assess effects at ontogenic, biochemical, and behavioral levels. The main developmental defects were tail deformities and pericardial edema at concentrations equal or above 0.32 mg/L. Cholinesterase activity (at 96 hpf) and larvae swimming behavior (at 120 hpf) were affected even at the lowest tested dose (0.02 mg/L). The behavior analysis was a sensitive endpoint, with a decrease in the swimming distance of exposed larvae during light period. The effect of 2,4-D in ChE was translated by an inhibition of the enzyme activity in all treated groups. These findings demonstrate that 2,4-D can alter the cholinergic system by affecting ChE activity which may be involved in the locomotion reduction of exposed larvae and emphasize the potential of neurotransmission and behavioral endpoints as early warning signs of herbicides contamination in aquatic ecosystems.

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Manganese(II) chloride alters behavioral and neurochemical parameters in larvae and adult zebrafish

Cited by 74 publications

References 57 publications

Pyriproxyfen Exposure Impairs Cognitive Parameters and Alters Cortisol Levels in Zebrafish

Pyriproxyfen Exposure Impairs Cognitive Parameters and Alters Cortisol Levels in Zebrafish

Evaluation of intestinal histological damage in zebrafish exposed to environmentally relevant concentrations of manganese

2,4-Dichlorophenoxyacetic acid herbicide effects on zebrafish larvae: development, neurotransmission and behavior as sensitive endpoints

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