Manganese(II) oxidation by the multi-copper oxidase CopA from Brevibacillus panacihumi MK-8

Zeng, Xin; Zhang, Mengtian; Liu, Yunying; Tang, Wenwei

doi:10.1016/j.enzmictec.2018.04.011

Cited by 25 publications

(13 citation statements)

References 25 publications

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“…Thus, the highly e cient manganese oxidation recombinant strain BL21-pET-copA has been successfully constructed and the target gene copA deletion recombinant plasmid has also been constructed by overlapping PCR. The copA gene-deleted strain (MK-8-ΔcopA) barely oxidized manganese, further demonstrating that the copA gene is the manganese oxidase gene (Zeng et al 2018).…”

Section: Introductionmentioning

confidence: 94%

“…The recombinant strain BL21-pET-copA has been successfully constructed in previous experiment (Zeng et al 2018). The strain was cultured in LB medium containing 1% (w/v) tryptone, 0.5% (w/v) yeast extract and 1% (w/v) sodium chloride and grown at 37℃ to an OD 600 ~0.6-0.8 supplemented with 10 mM Tris-HCl pH7.2 and 50 µg/mL kanamycin.…”

Section: Strain Media and Growth Conditionsmentioning

confidence: 99%

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Effect of Cu(II) and Conserved Copper Binding Sites on Multicopper Oxidase CopA and Characterization of the BioMnOx

Tang

Jin

Liu

et al. 2022

Preprint

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The microbial manganese removal process is believed to be the catalytic oxidation of Mn(II) by manganese oxidase. In this study, the multicopper oxidase CopA was purified and found to have high manganese oxidation activity in vitro and Cu(II) can significantly enhance its manganese oxidation activity. The gene site-directed mutagenesis was used to mutate four conserved copper binding sites of CopA and then obtain four mutant strains. The manganese removal efficiency of the four strains was determined to find that H120 is the catalytic active site of the CopA. Protein modification analysis of CopA obtained under different conditions by mass spectrometry revealed that the loss of Cu(Ⅱ) and the mutation of the conserved copper binding site H120 resulted in the loss of modification of ethoxyformyl and quinone, the number of modifications was reduced and the position of modification was changed, eventually causing a decrease in protein activity. It reveals that Cu(II) and H120 play an indispensable role in the manganese oxidation of the multicopper oxidase CopA. The Mn valence state of BioMnOx was analyzed by XPS, finding that both the strain-mediated product and the CopA-mediated product were composed of MnO2 and Mn3O4 and the average valence of Mn is 3.2.

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Section: Introductionmentioning

confidence: 94%

Section: Strain Media and Growth Conditionsmentioning

confidence: 99%

Effect of Cu(II) and Conserved Copper Binding Sites on Multicopper Oxidase CopA and Characterization of the BioMnOx

Tang

Jin

Liu

et al. 2022

Preprint

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“…Multicopper oxidase type enzymes CumA, MnxG, MofA, and McoA in some model organisms were responsible for Mn(II) oxidation [96][97][98]. Geszvain et al [99] found that after deleting either MnxG or MofA, the strains were still able to oxidize Mn(II), while oxidation was completely lost upon removal of both MnxG and MofA genes.…”

Section: Enzyme Driven Direct Oxidationmentioning

confidence: 99%

Removal of Manganese(II) from Acid Mine Wastewater: A Review of the Challenges and Opportunities with Special Emphasis on Mn-Oxidizing Bacteria and Microalgae

et al. 2019

Water

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Many global mining activities release large amounts of acidic mine drainage with high levels of manganese (Mn) having potentially detrimental effects on the environment. This review provides a comprehensive assessment of the main implications and challenges of Mn(II) removal from mine drainage. We first present the sources of contamination from mineral processing, as well as the adverse effects of Mn on mining ecosystems. Then the comparison of several techniques to remove Mn(II) from wastewater, as well as an assessment of the challenges associated with precipitation, adsorption, and oxidation/filtration are provided. We also critically analyze remediation options with special emphasis on Mn-oxidizing bacteria (MnOB) and microalgae. Recent literature demonstrates that MnOB can efficiently oxidize dissolved Mn(II) to Mn(III, IV) through enzymatic catalysis. Microalgae can also accelerate Mn(II) oxidation through indirect oxidation by increasing solution pH and dissolved oxygen production during its growth. Microbial oxidation and the removal of Mn(II) have been effective in treating artificial wastewater and groundwater under neutral conditions with adequate oxygen. Compared to physicochemical techniques, the bioremediation of manganese mine drainage without the addition of chemical reagents is relatively inexpensive. However, wastewater from manganese mines is acidic and has low-levels of dissolved oxygen, which inhibit the oxidizing ability of MnOB. We propose an alternative treatment for manganese mine drainage that focuses on the synergistic interactions of Mn in wastewater with co-immobilized MnOB/microalgae.

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“…The results here support the hypothesis that isolate SK3 also shares the activity of MCO enzyme in Mn(II) oxidation, as was reported with Ps. putida GB-1 [7] as well as in other genera, such as Bacillus [4], Brevibacillus [5], and Leptothrix [6].…”

Section: Isolation Of Pseudomonas Sp Sk3 and Its Phylogenetic Analysismentioning

confidence: 99%

Natural Attenuation of Mn(II) in Metal Refinery Wastewater: Microbial Community Structure Analysis and Isolation of a New Mn(II)-Oxidizing Bacterium Pseudomonas sp. SK3

Kitjanukit

Takamatsu

Okibe

2019

Water

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Natural attenuation of Mn(II) was observed inside the metal refinery wastewater pipeline, accompanying dark brown-colored mineralization (mostly MnIVO2 with some MnIII2O3 and Fe2O3) on the inner pipe surface. The Mn-deposit hosted the bacterial community comprised of Hyphomicrobium sp. (22.1%), Magnetospirillum sp. (3.2%), Geobacter sp. (0.3%), Bacillus sp. (0.18%), Pseudomonas sp. (0.03%), and non-metal-metabolizing bacteria (74.2%). Culture enrichment of the Mn-deposit led to the isolation of a new heterotrophic Mn(II)-oxidizer Pseudomonas sp. SK3, with its closest relative Ps. resinovorans (with 98.4% 16S rRNA gene sequence identity), which was previously unknown as an Mn(II)-oxidizer. Oxidation of up to 100 mg/L Mn(II) was readily initiated and completed by isolate SK3, even in the presence of high contents of MgSO4 (a typical solute in metal refinery wastewaters). Additional Cu(II) facilitated Mn(II) oxidation by isolate SK3 (implying the involvement of multicopper oxidase enzyme), allowing a 2-fold greater Mn removal rate, compared to the well-studied Mn(II)-oxidizer Ps. putida MnB1. Poorly crystalline biogenic birnessite was formed by isolate SK3 via one-electron transfer oxidation, gradually raising the Mn AOS (average oxidation state) to 3.80 in 72 h. Together with its efficient in vitro Mn(II) oxidation behavior, a high Mn AOS level of 3.75 was observed with the pipeline Mn-deposit sample collected in situ. The overall results, including the microbial community structure analysis of the pipeline sample, suggest that the natural Mn(II) attenuation phenomenon was characterized by robust in situ activity of Mn(II) oxidizers (including strain SK3) for continuous generation of Mn(IV). This likely synergistically facilitated chemical Mn(II)/Mn(IV) synproportionation for effective Mn removal in the complex ecosystem established in this artificial pipeline structure. The potential utility of isolate SK3 is illustrated for further industrial application in metal refinery wastewater treatment processes.

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Manganese(II) oxidation by the multi-copper oxidase CopA from Brevibacillus panacihumi MK-8

Cited by 25 publications

References 25 publications

Effect of Cu(II) and Conserved Copper Binding Sites on Multicopper Oxidase CopA and Characterization of the BioMnOx

Effect of Cu(II) and Conserved Copper Binding Sites on Multicopper Oxidase CopA and Characterization of the BioMnOx

Removal of Manganese(II) from Acid Mine Wastewater: A Review of the Challenges and Opportunities with Special Emphasis on Mn-Oxidizing Bacteria and Microalgae

Natural Attenuation of Mn(II) in Metal Refinery Wastewater: Microbial Community Structure Analysis and Isolation of a New Mn(II)-Oxidizing Bacterium Pseudomonas sp. SK3

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