Mangiferin attenuates cisplatin-induced acute kidney injury in rats mediating modulation of MAPK pathway

Abstract

Cisplatin has been confined due to the reported cases of nephrotoxicity. In the present study, an active xanthone, Mangiferin (from Mangifera indica) was investigated for its defensive role in cisplatin-induced nephrotoxicity. Male wistar albino rats were divided into six groups i.e., group 1 (normal); group 2 (cisplatin control); group 3, 4, and 5 (mangiferin 10, 20, and 40 mg/kg, i.p.); and per se (40 mg/kg; i.p.). The treatment was given for 10 days. On day 7, single dose of cisplatin 8 mg/kg i.p. was administered to induce nephrotoxicity in all groups except normal and per se. On day 11, animals were anesthetized, blood was taken from heart and serum was separated. Thereafter, rats were sacrificed and kidneys were isolated and preserved for histopathological, ultrastructural, immunohistochemical, and western blot analysis. Cisplatin control group showed significant impairment in renal function due to increased inflammation and oxidative stress which was also confirmed by histopathology and MAPK pathway proteins expression. However, pretreatment with mangiferin 20 and 40 mg/kg significantly reversed the renal function along with the structural changes and the levels of antioxidants. Mangiferin treatment attenuated DNA damage and apoptotic pathway.

Characterization of protein extracts from different types of human teeth and insight in biomineralization

“…4C). Finally, to remove collagen biased mineralization effects, a cisplatin induced nephrotoxic rat kidney tissue with elevated collagen content 41–43 (confirmed by rat collagen I ELISA kit, see Supplementary Fig. 6) was also analyzed by mineralization experiments followed by NTA size measurements at different time points (Supplementary Fig.…”

Acute Effects of a Polyphenol-Rich Leaf Extract of Mangifera indica L. (Zynamite) on Cognitive Function in Healthy Adults: A Double-Blind, Placebo-Controlled Crossover Study

“…Increased acetylcholine activity would be expected to have a beneficial, inter-related effect on both focussed attention and memory consolidation/retrieval [64] and, therefore, could encompass many of the effects seen in the current study. However, it is equally likely that the effects seen here may be related to 'indirect' interactions within mammalian cellular signal transduction pathways, a property that mangiferin shares with other polyphenols [8,[11][12][13][14]. These interactions potentially drive downstream modulation of neuroinflammation, neurotransmission, neurotrophin receptor and signalling pathway interactions, and increased synthesis of neurotrophins and vasodilatory molecules, leading to increased angiogenesis/neurogenesis and local cerebral blood flow [15][16][17][18][19].…”

“…Whilst structurally distinct from the flavonoids and other polyphenols that are ubiquitous in plant derived foods, mangiferin [8,[11][12][13][14] likely owes its beneficial bioactivity to some similar mechanisms of action as found in the wider polyphenol group class [15], including interactions with, and modulation of, diverse components of a wide range of mammalian cellular signal transduction pathways. These pathways, in turn, control gene transcription and a plethora of cellular responses, including cell proliferation, apoptosis, and the synthesis of growth factors, and vasodilatory and inflammatory molecules.…”

Cordyceps cicadae Mycelia Ameliorate Cisplatin-Induced Acute Kidney Injury by Suppressing the TLR4/NF-κB/MAPK and Activating the HO-1/Nrf2 and Sirt-1/AMPK Pathways in Mice

“…Sections with a thickness of 5 μm were cut and stained with H&E, then photographed using light microscopy (Nikon Eclipse TS100, Japan). Renal injury was scored based on the percentage of epithelial necrosis in the cortical tubules: 0, normal kidney; 1, <25% damage; 2, 25-50% damage; 3, 50-75% damage; and 4, >75% damage [21].…”