Mango explant browning: Effect of ontogenic age, mycorrhization and pre-treatments

Krishna, Hare; Sairam, R. K.; Singh, S. K.; Patel, V.B.; Sharma, R.R.; Grover, Monendra; Nain, Lata; Sachdev, Archana

doi:10.1016/j.scienta.2008.05.040

Cited by 74 publications

(50 citation statements)

References 28 publications

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“…4). Similar to our findings, Krishna et al (2008) reported a positive correlation between phenol exudation and necrosis of explants. Thus, AOPP controls phenol content and medium browning for 4 weeks from the start of culture, increasing the plant regeneration potential.…”

Section: Discussionsupporting

confidence: 92%

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L-2-aminooxy-3-phenylpropionic acid (AOPP) Controls Browning and Promotes Adventitious Bud Formation in Neofinetia falcata in vitro

Mitsukuri

Johkan

Yamasaki

et al. 2010

J. Japan. Soc. Hort. Sci.

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The effect of L-2-aminooxy-3-phenylpropionic acid (AOPP) on bud formation was investigated by determination of total phenolics in explants and medium browning in the micropropagation of the epiphytic orchid Neofinetia falcata (Thunb.) H.H. Hu. Explants, upper portion of plantlets, were cultured on a medium containing 0, 0.01, 0.1 or 1.0 mM AOPP. Browning was decreased from 70% to 3% by increasing AOPP concentration up to 1.0 mM. All surviving explants developed buds at 0, 0.01 and 0.1 mM AOPP. The highest mean number of buds (7.2) was produced on medium containing 0.01 mM AOPP. Multiple buds were formed on the explants 8 weeks after planting and multiple shoots had developed after 12 weeks. Total phenolics continuously increased in control explants up to 2.03 mg·gFW −1 after 4 weeks. In contrast, in explants treated with 0.01 mM AOPP, total phenolics ceased to increase for 4 weeks of culture and remained at 1.46 mg·gFW −1 , but thereafter increased to 2.24 mg·gFW −1 , the same level as the control, after 8 weeks. Medium browning continuously increased in the control, whereas medium containing 0.01 mM AOPP showed little browning after 4 weeks of culture and thereafter increased to the same degree as the control. The present results suggest that 0.01 mM AOPP is the most suitable concentration to control browning and bud formation in N. falcata.

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Section: Discussionsupporting

confidence: 92%

“…Medium browning was measured using a modified protocol (Krishna et al, 2008;Park et al, 2006). Media (5.0 g) sampled from two culture vessels were homogenized in a mortar with 80% ethyl alcohol.…”

Section: Medium Browningmentioning

confidence: 99%

L-2-aminooxy-3-phenylpropionic acid (AOPP) Controls Browning and Promotes Adventitious Bud Formation in Neofinetia falcata in vitro

Mitsukuri

Johkan

Yamasaki

et al. 2010

J. Japan. Soc. Hort. Sci.

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“…In the reaction, the H 2 O 2 produced is scavenged by catalase (CAT) and peroxidase (POD), reducing the formation of hydroxyl radicals (OH˙) (Scandalios, 1993). Finally, polyphenoloxidase (PPO) catalyses the O 2 -dependent oxidation of catechols in quinones, and may synergistically act with POD, promoting its activation through the generation of H 2 O 2 by the oxidation of phenolic compounds (Krishna et al, 2008). Although less studied, PPO is an abundant plant enzyme (Agrawal and Purohit, 2012).…”

Section: Introductionmentioning

confidence: 99%

Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

et al. 2016

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This study aimed to evaluate the variation in the levels of proline, oxidative metabolism and photosynthetic pigments in plants of Pitcairnia encholirioides grown in vitro under different conditions and after acclimatization. The analyses were performed after 150 days of in vitro cultivation in MS media supplemented with 10 µM GA 3 or 0.2 µM NAA, sucrose at 15 or 30 g L -1 , in test tubes which allowed gas exchange or in a hermetically sealed system, and 180 days after acclimatization. The in vitro maintenance in hermetically sealed flasks, with GA 3 and 15 g L -1 sucrose had adverse metabolic effects, which was demonstrated by the lower proline and photosynthetic pigments accumulation and by the increase in antioxidant enzymes activities. After acclimatization, differences for proline and photosynthetic pigments were no longer found and the enzymatic activities ranged unevenly. The results suggest that the in vitro cultivation in media with 0.2 µM NAA and 30 g L -1 sucrose, in test tubes capped with closures which allowed gas exchange, is more suitable for micropropagation of P. encholirioides, providing a prolonged maintenance of in vitro cultures and plantlets with superior quality for ex vitro development.Keywords: enzymatic activity, oxidative stress, micropropagation, proline. Niveis de prolina, do metabolismo oxidativo e dos pigmentos fotossintéticos durante o crescimento in vitro e aclimatização de Pitcairnia encholirioides L.B. Sm. (Bromeliaceae) ResumoEste trabalho objetivou avaliar a contribuição da prolina, do metabolismo oxidativo e dos pigmentos fotossintéticos na propagação in vitro e aclimatização de Pitcairnia encholirioides, uma bromélia criticamente ameaçada de extinção. As análises foram realizadas após 150 dias de cultivo in vitro em meio MS suplementado com 10 µM de GA 3 ou 0,2 µM de ANA, 15 ou 30 g L -1 de sacarose, em tubos de ensaio que permitiam trocas gasosas ou em sistema hermeticamente vedado, e também 180 dias após aclimatização. A manutenção in vitro em frascos hermeticamente fechados, com GA 3 e 15 g L -1 de sacarose apresentou efeito metabólico adverso, demonstrado pelo menor acúmulo de prolina e pigmentos fotossintéticos e também pelo aumento das atividades de enzimas antioxidantes. Após aclimatização, as diferenças para prolina e pigmentos fotossintéticos não foram mais encontradas e as atividades enzimáticas variaram de maneira desuniforme. Os resultados sugerem que o cultivo in vitro em meio com 0,2 µM de ANA e 30 g L -1 de sacarose, em tubos fechados com tampas que permitem trocas gasosas, é mais adequado para a micropropagação de P. encholirioides, proporcionando uma manutenção prolongada das culturas in vitro e plântulas com qualidade superior para o desenvolvimento ex vitro.Palavras-chave: atividade enzimática, estresse oxidativo, micropropagação, prolina.

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“…L-Ascorbic acid (100 mg/l) and 0.3 % PVP (polyvinylpyrrolidone) were added in the induction and conversion media to control explants browning (Mishra et al, 2010) along with frequent sub-culturing and incubation in dark conditions to minimize the effects of phenolic exudations (Ara et al, 2000). Pre-treatment of explants with antioxidants solutions (Hare Krishna et al, 2008), pre-culture in liquid induction media (Li et al, 2012), use of activated charcoal (0.5 gm/l) in basal media, incubation of nucellar cultures in dark with frequent sub culturing (Litz, 2003) are strategies which were found to be effective for prevention of browning of explants…”

Section: Explant Surface Sterilization and Treatmentsmentioning

confidence: 99%

In vitro regeneration of Mango (Mangifera indica L.) cv. Baramasi through nucellar embryogenesis

Al-Busaidi¹,

Shukla²,

Al-Burashdi³

et al. 2016

J. Hortic. For.

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Vegetative propagation via grafting is a common method to propagate mono-embryonic mango (Mangifera indica L.) varieties in Oman which is time consuming and expensive. Standardize in-vitro regeneration protocol in producing true-to-type, disease free and homogeneous high quality plants is prerequisite. Nucellar tissues from immature mango fruits of mono-embryonic cv. Baramasi were used as explants to induce somatic embryogenesis and plantlets regeneration. Several media compositions were evaluated for all the four stages that is, induction, conversion, maturation and germination. Modified Gamborg's B5 major salts, MS minor salts, iron source and organics were used as basal media with varying hormone concentrations. The highest number of callused explants (12.6) was observed in induction media 1 (IND1) containing 1 mg/l 2, 4-D, 0.25 mg/l BAP, 400 mg/l L-glutamine and 500 mg/l malt extract. About 49.6% callus produced somatic embryos (SEs) and maximum 69.7 SEs were proliferated from each embryogenic callus in conversion media 2 (CM2) having 0.5 mg/l BAP. The highest germination (35.9%) with well-developed shoot, leaves and roots was observed in germination media 5 (GM5) containing 0.1 mg/l IAA, 1 mg/l Kinetin and 0.5 mg/l GA3. About 65% of transplanted plants are still surviving in green house conditions even after 4 months of transfer.

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Mango explant browning: Effect of ontogenic age, mycorrhization and pre-treatments

Cited by 74 publications

References 28 publications

L-2-aminooxy-3-phenylpropionic acid (AOPP) Controls Browning and Promotes Adventitious Bud Formation in Neofinetia falcata in vitro

L-2-aminooxy-3-phenylpropionic acid (AOPP) Controls Browning and Promotes Adventitious Bud Formation in Neofinetia falcata in vitro

Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

In vitro regeneration of Mango (Mangifera indica L.) cv. Baramasi through nucellar embryogenesis

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