Mangrovibacter plantisponsor gen. nov., sp. nov., a nitrogen-fixing bacterium isolated from a mangrove-associated wild rice (Porteresia coarctata Tateoka)

Abstract

Mangrovibacter plantisponsor gen. nov., sp. nov., a nitrogen-fixing bacterium isolated from a mangrove-associated wild rice (Porteresia coarctata Tateoka A facultatively anaerobic, nitrogen-fixing bacterium, strain MSSRF40 T , was isolated from roots of mangrove-associated wild rice (Porteresia coarctata Tateoka). On the basis of 16S rRNA gene sequence similarities, strain MSSRF40 T was shown to belong to the family Enterobacteriaceae, most closely related to Cronobacter muytjensii E603 T (97.2 % sequence similarity), Enterobacter cloacae subsp. dissolvens LMG 2683 T (97.1 %), E. radicincitans D5/23 T (97.1 %) and E. ludwigii EN-119 T (97.0 %). Sequence analysis of rpoB, gyrB and hsp60 genes showed that strain MSSRF40 T had relatively low sequence similarity (,91, ,84 and ,90 %) to recognized species of different genera of the family Enterobacteriaceae and formed an independent phyletic lineage in all phylogenetic analyses using the 16S rRNA, rpoB, gyrB and hsp60 genes, clearly indicating that strain MSSRF40 T could not be affiliated to any of the recognized genera within the family Enterobacteriaceae. The dominant cellular fatty acids were C 16 : 0 , C 16 : 1 v7c and/or iso-C 15 : 0 2-OH and C 18 : 1 v7c, similar to those of other members of the Enterobacteriaceae. The DNA G+C content was 50.1 mol%. Phylogenetic distinctiveness and phenotypic differences from its phylogenetic neighbours indicated that strain MSSRF40 T represents a novel species and genus within the family Enterobacteriaceae, for which the name Mangrovibacter plantisponsor gen. nov., sp. nov. is proposed. The type strain of Mangrovibacter plantisponsor is strain MSSRF40 T (5LMG 24236 T 5DSM 19579 T ).The family Enterobacteriaceae is a large, heterogeneous group of Gram-negative, facultatively anaerobic, rodshaped bacteria that do not form endospores and are catalase-positive and oxidase-negative (Brenner, 1984). Members of the family are distributed worldwide, with some being saprophytes and others being parasites of plants and animals. Many species of this family are of considerable economic importance due to their pathogenic effects on agriculture and livestock (Janda & Abbott, 2006). Currently, there are more than 35 genera described in this family (Dauga, 2002;Janda, 2006), including the recently described genus Cronobacter (Iversen et al., 2008), which was previous recognized as Farmer's Enterobacter sakazakii biogroups 1-4, 7, 8, 11 and 13 (Iversen et al., 2007, 2008).Several studies have shown that members of the Enterobacteriaceae may have beneficial effects on plant development when they are associated with the plants as rhizobacteria (Lodewyckx et al., 2002;Taghavi et al., 2009). They improve plant growth via nitrogen fixation, suppression of plant pathogens and production of phytohormones and enzymes involved in the metabolism of growth regulators such as ethylene, 1-aminocyclopropane 1-carboxylic acid (ACC), auxins and indole-3-acetic acid (IAA) (Gyaneshwar et al., 2001; Kämpfer et al., 2005;Taghavi et al., 2009).During a study of di...

Budvicia diplopodorum sp. nov. and emended description of the genus Budvicia

“…The component C 17 : 0 was found in three members of the genus Yersinia tested in this study and has been reported for the genus Yersinia and other genera within the family Enterobacteriaceae by other authors (Jantzen & Lassen, 1980;Kämpfer et al, 2005;Whittaker et al, 2005;Madhaiyan et al, 2010;Rameshkumar et al, 2010).…”

“…The amplification, sequencing and phylogenetic analysis of the three genes were carried out as described previously (Rameshkumar et al, 2010 T in the genus Budvicia. These data revealed that rpoB, gyrB and hsp60 sequences have a high phylogenetic resolution for species identification in the family Enterobacteriaceae.…”

Transfer of Pseudomonas flectens Johnson 1956 to Phaseolibacter gen. nov., in the family Enterobacteriaceae , as Phaseolibacter flectens gen. nov., comb. nov.

“…For example, the type strain of Mangrovibacter plantisponsor (Rameshkumar et al, 2010) shares 97.2 % 16S rRNA gene sequence similarity with Cronobacter muytjensii E603 T and 97.1 % similarity with Enterobacter cloacae subsp. dissolvens LMG 2683…”

“…Grows on MacConkey agar. The following results are obtained from API 20E strips after 48 h of incubation at 28 u C: Larabinose fermentation is variable (negative for the type strain), D-mannitol, inositol, sorbitol, rhamnose and amygdalin are not fermented; acetoin is produced Brady et al, 2010;Brenner & Farmer, 2005;Hollis et al, 1981); 3, Pantoea (Pantoea agglomerans DSM 3493 T : Brenner & Farmer, 2005;Gavini et al, 1989;Kageyama et al, 1992;Mergaert et al, 1993); 4, Leclercia (Leclercia adecarboxylata DSM 30081 T : Brenner & Farmer, 2005;Rameshkumar et al, 2010;Tamura et al, 1986); 5, Raoultella (Raoultella planticola DSM 3096 T : Brenner & Farmer, 2005;Drancourt et al, 2001;Gavini et al, 1986) Brenner & Farmer, 2005;Grimont & Grimont, 2006;Rameshkumar et al, 2010); 10, Cedecea (Cedecea davisae DSM 4568…”

Rosenbergiella nectarea gen. nov., sp. nov., in the family Enterobacteriaceae, isolated from floral nectar

“…For example, the type strain of Mangrovibacter plantisponsor (Rameshkumar et al, 2010) shares 97.2 % 16S rRNA gene sequence similarity with Cronobacter muytjensii E603 T . Moreover, the type strains of the type species of Klebsiella and Raoultella, which were described as two different genera (Drancourt et al, 2001), share 98.0 % 16S rRNA gene sequence similarity.…”