Manipulating the Gibberellin Response to Reduce Plant Height in Chrysanthemum Morifolium

Petty, L. M.; Harberd, Nicholas P.; Thomas, Brian; Jackson, Stephen D.

doi:10.17660/actahortic.2001.560.9

Cited by 7 publications

(5 citation statements)

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“…Also, antisense silencing of the GA biosynthesis gene GA20-oxidase resulted in smaller leaves, delayed flowering time and reduced fertility in Arabidopsis [8]. Overexpression of the Arabidopsis GA signalling gene GIBBERELLIN INSENSITIVE ( GAI ) in apple and Chrysanthemum reduced plant height, but was correlated with reduced rooting ability and delayed flowering in the respective species [7], [22]. In light of these observations, introduction of the AtSHI gene to poinsettia might be highly interesting as a means to control elongation growth without introducing undesired morphological or developmental changes.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of the AtSHI Gene in Poinsettia, Euphorbia pulcherrima, Results in Compact Plants

et al. 2013

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Euphorbia pulcherrima, poinsettia, is a non-food and non-feed vegetatively propagated ornamental plant. Appropriate plant height is one of the most important traits in poinsettia production and is commonly achieved by application of chemical growth retardants. To produce compact poinsettia plants with desirable height and reduce the utilization of growth retardants, the Arabidopsis SHORT INTERNODE (AtSHI) gene controlled by the cauliflower mosaic virus 35S promoter was introduced into poinsettia by Agrobacterium-mediated transformation. Three independent transgenic lines were produced and stable integration of transgene was verified by PCR and Southern blot analysis. Reduced plant height (21–52%) and internode lengths (31–49%) were obtained in the transgenic lines compared to control plants. This correlates positively with the AtSHI transcript levels, with the highest levels in the most dwarfed transgenic line (TL1). The indole-3-acetic acid (IAA) content appeared lower (11–31% reduction) in the transgenic lines compared to the wild type (WT) controls, with the lowest level (31% reduction) in TL1. Total internode numbers, bract numbers and bract area were significantly reduced in all transgenic lines in comparison with the WT controls. Only TL1 showed significantly lower plant diameter, total leaf area and total dry weight, whereas none of the AtSHI expressing lines showed altered timing of flower initiation, cyathia abscission or bract necrosis. This study demonstrated that introduction of the AtSHI gene into poinsettia by genetic engineering can be an effective approach in controlling plant height without negatively affecting flowering time. This can help to reduce or avoid the use of toxic growth retardants of environmental and human health concern. This is the first report that AtSHI gene was overexpressed in poinsettia and transgenic poinsettia plants with compact growth were produced.

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Section: Introductionmentioning

confidence: 99%

Overexpression of the AtSHI Gene in Poinsettia, Euphorbia pulcherrima, Results in Compact Plants

et al. 2013

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“…Ectopic expression of gai in Chrysanthemum also produced dwarfed plants. However, both the number and size of the flowers were reduced and the transgenic plants had delayed flowering time (Petty et al. , 2001).…”

Section: Introductionmentioning

confidence: 99%

Production of compact plants by overexpression of AtSHI in the ornamental Kalanchoë

Lütken

Jensen

Topp

et al. 2010

Plant Biotechnology Journal

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SummaryGrowth retardation is an important breeding aim and an essential part of horticultural plant production. Here, the potential of transferring the Arabidopsis short inter- ing a widespread presence of this transcription factor. These findings are important because they suggest that transformation with the AtSHI gene could be applied to several species as a tool for growth retardation, and that this approach could substitute the use of conventional chemical growth regulation in plant production.

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“…Also, antisense silencing of the GA biosynthesis gene GA20-oxidase resulted in smaller leaves, delayed flowering time and reduced fertility in Arabidopsis [8]. Overexpression of the Arabidopsis GA signalling gene GIBBERELLIN INSENSITIVE (GAI) in apple and Chrysanthemum reduced plant height, but was correlated with reduced rooting ability and delayed flowering in the respective species [7,22]. In light of these observations, introduction of the AtSHI gene to poinsettia might be highly interesting as a means to control elongation growth without introducing undesired morphological or developmental changes.…”

Section: Introductionmentioning

confidence: 99%

Correction: Overexpression of the AtSHI Gene in Poinsettia, Euphorbia pulcherrima, Results in Compact Plants

Islam¹,

Lütken²,

Haugslien³

et al. 2013

PLoS ONE

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Euphorbia pulcherrima, poinsettia, is a non-food and non-feed vegetatively propagated ornamental plant. Appropriate plant height is one of the most important traits in poinsettia production and is commonly achieved by application of chemical growth retardants. To produce compact poinsettia plants with desirable height and reduce the utilization of growth retardants, the Arabidopsis SHORT INTERNODE (AtSHI) gene controlled by the cauliflower mosaic virus 35S promoter was introduced into poinsettia by Agrobacterium-mediated transformation. Three independent transgenic lines were produced and stable integration of transgene was verified by PCR and Southern blot analysis. Reduced plant height (21-52%) and internode lengths (31-49%) were obtained in the transgenic lines compared to control plants. This correlates positively with the AtSHI transcript levels, with the highest levels in the most dwarfed transgenic line (TL1). The indole-3-acetic acid (IAA) content appeared lower (11-31% reduction) in the transgenic lines compared to the wild type (WT) controls, with the lowest level (31% reduction) in TL1. Total internode numbers, bract numbers and bract area were significantly reduced in all transgenic lines in comparison with the WT controls. Only TL1 showed significantly lower plant diameter, total leaf area and total dry weight, whereas none of the AtSHI expressing lines showed altered timing of flower initiation, cyathia abscission or bract necrosis. This study demonstrated that introduction of the AtSHI gene into poinsettia by genetic engineering can be an effective approach in controlling plant height without negatively affecting flowering time. This can help to reduce or avoid the use of toxic growth retardants of environmental and human health concern. This is the first report that AtSHI gene was overexpressed in poinsettia and transgenic poinsettia plants with compact growth were produced.

show abstract

Manipulating the Gibberellin Response to Reduce Plant Height in Chrysanthemum Morifolium

Cited by 7 publications

References 0 publications

Overexpression of the AtSHI Gene in Poinsettia, Euphorbia pulcherrima, Results in Compact Plants

Overexpression of the AtSHI Gene in Poinsettia, Euphorbia pulcherrima, Results in Compact Plants

Production of compact plants by overexpression of AtSHI in the ornamental Kalanchoë

Correction: Overexpression of the AtSHI Gene in Poinsettia, Euphorbia pulcherrima, Results in Compact Plants

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