Manipulation of particles based on swirl

Abstract

A method of manipulating particles based on swirl was proposed in this article. The pressure and velocity distributions of swirling flow field were analyzed theoretically. The mechanism of particle trapping and controlling based on swirling flow was discussed, and the feasibility of the proposed method was verified by experiments. The results show that a swirling flow can be generated by placing two microtubules in parallel and jetting fluid in opposite directions. Particles with arbitrary size and shape can be trapped and controlled to rotate and move directionally and quantitatively with proper swirling flow parameters.

A Review of Single‐Cell Pose Adjustment and Puncture

“…Optical field [36,[103][104][105][106][107]109,115,167] Trapping D, ξ, δ Medium, High 4-20 High (0.0001 % 0.001) High rotation accuracy, label-free, single-cell, low drift Optical damage, expensive instrumentation, weak capture low throughput, bulky optical system with opaque Magnetic field [8,119,120,125,126,128,133,134,168] Magnetic gradient D, χ High 5-120 Higher (0.0001 % 0.01) Greater drive power, reliability and efficiency Constrained by the magnetic field distribution, requires pretreatment of cells, labeling, force hysteresis Acoustic field [138,139,[141][142][143][144][145][146]155,169] Axial acoustic forces D, ρ, β Low 10-3100 Low (1 % 10) Label-free Stimulation of cells, required piezoelectric substrates for chip fabrication, limited precise cell rotation Low cost, massive, article rotation Hydrodynamic drive [64,156,160,[160][161][162][163][164][165] Hydrodynamic inertial forces D Low 10-5 50 Low (1 % 10) Easy to integrate manufacturing Rotational accuracy, complex and cumbersome external control equipment, poor flexibility, difficult to control specific single-cell a)…”

“…Stable cell rotation requires control of the flow rate in the pipette to generate laminar fluid (Figure 7c), to achieve out-of-plane and in-plane rotation with a cell rotation accuracy of 1.9°under a visual feedback system. On that basis, Zhang et al [47,161,162] systematically explored the vortex phenomenon formed by dualpipette and three-pipette spray fluids (Figure 7d), which arose from the directional motion formed by the fluid. Furthermore, the cells of different shapes and sizes, ranging from a few micrometers to several hundred micrometers in size, could be captured using the vortex stationary points.…”

“…[64] d) Double-tube and triple-tube spray of fluid to form vortices. [47,161,162] e) Schematic of the multifunctional manipulation using whirling flow generated by vibration of a single-piezo actuator. [163] Mechanism of the multifunctional micromanipulation, immobilization and transportation in Y-and X-axis, rotation.…”

“…Leung et al [64] 2012 Single-tube spray fluid 3 22.8 cell s À1 Mouse oocytes 50 Polar body position Zhang et al [161] 2017 Double microtube-induced cyclonic flow 1 0.0147 m s Shrimp egg, particle 100-550 Cell rotation…”

“…c) A standard suction-holding needle generating layers of fluid [64]. d) Double-tube and triple-tube spray of fluid to form vortices [47,161,162]. e) Schematic of the multifunctional manipulation using whirling flow generated by vibration of a single-piezo actuator [163].…”