Manipulation of rhizobia microflora for improving legume productivity and soil fertility: A critical assessment

Brockwell, J; Bottomley, Peter J.; Thies, Janice E.

doi:10.1007/bf00032245

Cited by 245 publications

(103 citation statements)

References 333 publications

(164 reference statements)

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“…In the second experiment, failure of inoculation to elicit response in cowpea and green gram could be attributed to the presence of effective indigenous rhizobia or highly competitive but ineffective indigenous strains that lock out the inoculant strains from occupying the nodules (Theuri et al 2006). Yield and nodulation are seldom increased by inoculation partly due to high population size of indigenous rhizobia in tropical soils (Kang 1977;Danso and Owiredu 1988;Thies et al 1995). This was confirmed by the MPN trial on the soil from Chonyi indicating rhizobial populations of >13.5 Â 10 3 CFU g…”

Section: Discussionsupporting

confidence: 51%

“…However, inoculation is not universal and inoculation does not always elicit positive responses. Inoculation of legumes is necessary in the absence of compatible rhizobia and when rhizobial populations are low or inefficient in fixing N (Brockwell et al 1995;Catroux et al 2001;Fening and Danso 2002;Abaidoo et al 2007). Use of rhizobia inoculants for soybeans (Glycine max L.…”

Section: Introductionmentioning

confidence: 99%

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Potential of indigenous bradyrhizobia versus commercial inoculants to improve cowpea (Vigna unguiculataL. walp.) and green gram (Vigna radiataL. wilczek.) yields in Kenya

Mathu

Herrmann

Pypers

et al. 2012

Soil Science and Plant Nutrition

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Limited information is available on reduced cowpea (Vigna unguiculata L. Walp.) and green gram (Vigna radiata L.Wilczek.) yields in Kenya. Declining soil fertility and absence or presence of ineffective indigenous rhizobia in soils are assumptions that have been formulated but still require to be demonstrated. In this study, soils were collected from legume growing areas of Western (Bungoma), Nyanza (Bondo), Eastern (Isiolo), Central (Meru) and Coast (Kilifi) provinces in Kenya to assess indigenous rhizobia in soils nodulating cowpea and green gram under greenhouse conditions. Our results showed that highest nodule fresh weights of 4.63 and 3.32 g plant À1 for cowpea and green gram were observed in one soil from Isiolo and another from Kilifi, respectively, suggesting the presence of significant infective indigenous strains in both soils. On the other hand, the lowest nodule fresh weights of 2.17 and 0.72 g plant À1 were observed in one soil from Bungoma for cowpea and green gram, respectively. Symbiotic nitrogen (N) fixation by cowpea and green gram was highest in Kilifi soil with values of 98% and 97%, respectively. A second greenhouse experiment was undertaken to evaluate the performance of commercial rhizobial inoculants with both legumes in Chonyi soil (also from Coast province) containing significant indigenous rhizobia [>13.5 Â 10 3 Colony Forming Units (CFU) g À1]. Rhizobial inoculation did not significantly (P < 0.05) affect nodulation, biomass yield and shoot N content in cowpea and green gram compared with controls. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the 16S-23S rDNA intergenic spacer (IGS) region analysis of nodules revealed six groups of which only IGS Group IV corresponded with those from commercial inoculants applied, indicating a lower competitiveness of inoculated strains. In cowpea, IGS III was dominant in nodules of plants inoculated with Biofix and Rizoliq commercial inoculants, and the uninoculated control treatment (63.2, 60 and 52.9%, respectively). Similarly, in green gram, IGS Group III was dominant in nodules of plants inoculated with Biofix 704 and Rizoliq commercial inoculants, and the uninoculated control treatment (75, 73.7 and 61.1%, respectively). Our results suggest that the systematic inoculation of both legumes with current available commercial inoculants to improve biomass yields is not necessary in these regions of Kenya. Also, according to our study, it would make sense to promote the utilization of indigenous strains performing well with both legumes.

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Section: Discussionsupporting

confidence: 51%

Section: Introductionmentioning

confidence: 99%

Potential of indigenous bradyrhizobia versus commercial inoculants to improve cowpea (Vigna unguiculataL. walp.) and green gram (Vigna radiataL. wilczek.) yields in Kenya

Mathu

Herrmann

Pypers

et al. 2012

Soil Science and Plant Nutrition

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“…Esses autores verificaram que em solos com população rizobiana elevada o aumento da taxa de inóculo favoreceu o ANPA em plantas de feijão-caupi. Contudo, Brockwell et al (1995) comentam que a inoculação em solos que apresentam população rizobiana estabelecida acima de 10 3 rizóbios g -1 de solo é viável, desde que a maioria dos rizóbios seja ineficiente para a leguminosa em estudo.…”

Section: Resultsunclassified

“…De acordo com Brockwell et al (1995), a melhor maneira de se estabelecer uma estirpe de rizóbio na presença de população rizobiana nativa é por meio da aplicação de taxa elevada de inóculo eficaz, colocandoo perto dos primeiros sítios de nodulação da plantano caso, a raiz. Krasova-Wade et al (2006) demonstraram que a densidade e competitividade de uma população rizobiana nativa podem ser modificadas por estirpes introduzidas pela aplicação de taxas de inóculo superiores à densidade da população rizobiana nativa.…”

Section: Introductionunclassified

Nodulação e eficiência da fixação do N2 em feijão-caupi por efeito da taxa do inóculo

Silva¹,

Santos

Sousa³

et al. 2012

Rev. Bras. Ciênc. Solo

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& Márcia do Vale Barreto Figueiredo (7)RESUMO O feijão-caupi (Vigna unguiculata (L.) Walp.) pode adquirir nitrogênio em quantidades adequadas para suprir suas necessidades, por meio do processo de fixação biológica do nitrogênio (FBN), quando associado com rizóbios específicos e eficientes. O objetivo deste trabalho foi avaliar o efeito da aplicação de diferentes taxas de inóculo na nodulação e FBN na cultivar BRS Pujante de feijão-caupi. O experimento foi conduzido em casa de vegetação, utilizando-se vasos plásticos com 2 kg de solo, em delineamento experimental de blocos ao acaso com sete tratamentos e quatro repetições. Os tratamentos constaram de cinco concentrações de células da estirpe de Bradyrhizobium sp. BR 3267 (células/semente), um tratamento sem inoculação e com adição de fertilizante nitrogenado (controle + N) e um controle sem inoculação e sem adição de fertilizante nitrogenado (controle). Foram avaliados o número e a massa seca de nódulos, a massa seca da parte aérea e o acúmulo de N na parte aérea. À medida que houve aumento da concentração de células na semente, elevaram-se os parâmetros da nodulação e fixação do N 2 . A aplicação da maior taxa de inóculo, que foi de 6,65 x 10 7 células da estirpe BR 3267/ semente, promoveu aumento da massa seca da parte aérea por planta correspondente a 27 % da massa seca da parte aérea do tratamento controle e semelhante ao tratamento controle + N. A inoculação a partir da aplicação de 8 x 10 5 células da estirpe BR 3267/semente forneceu maior quantidade de N para as plantas, em relação à dos tratamentos controle e controle + N. A cultivar BRS Pujante foi

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“…Although many other factors could be considered in selecting the strains that will be used in commercial inoculants (see Brockwell et al 1995), several of the strains in this study look to be promising candidates based on their effectiveness and soil persistence. An important character not yet assessed, however, is genetic stability.…”

Section: Discussionmentioning

confidence: 99%

Selecting improved Lotus nodulating rhizobia to expedite the development of new forage species

et al. 2011

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Aims In the past decades the increasing focus by Australian pasture development programs on the genus Lotus has seen the evaluation of many species previously untested in Australia. In field trials, nodulation failure was commonplace. This work was undertaken to select effective symbionts for Lotus to ensure further agronomic evaluation of the genus was not compromised. The symbiotic needs of Lotus ornithopodioides were a particular focus of the studies.Methods Glasshouse experiments were undertaken to evaluate symbiotic relationships between 15 Lotus spp and 23 strains of nodulating Mesorhizobium loti. This was followed by evaluation of elite rhizobial strains for their ability to persist and form nodules under field conditions. Results Complex symbiotic interactions were recorded between strains of lotus rhizobia and the different species of Lotus. Notably, the rhizobia that are currently provided commercially in Australia for the inoculation of Lotus corniculatus (strain SU343) and Lotus uliginosus (strain CC829) did not form effective symbioses with the promising species L. ornithopodioides and L. maroccanus. No strain we evaluated was compatible with all the Lotus species, however several strains with a broad host range were identified. WSM1293 and WSM1348 were the most effective strains on L. ornithopodioides and L. peregrinus.These strains were also moderately effective on L. corniculatus (79 and 52% of SU343), less effective on L. maroccanus (26 and 49% of SRDI110) but were ineffective on L. uliginosus. The latter species overall had very specific rhizobial needs. Both WSM1293 and WSM1348 produced adequate levels of nodulation when inoculated on L. ornithopodioides, over two seasons at three field sites. Conclusions Effective and persistent strains are now available that should allow the un-compromised evaluation of many of the contemporary Lotus species in the field. Selecting a strain for use in commercial inoculants will be more problematic, given the very Plant Soil (2011) 348:231-243

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Manipulation of rhizobia microflora for improving legume productivity and soil fertility: A critical assessment

Cited by 245 publications

References 333 publications

Potential of indigenous bradyrhizobia versus commercial inoculants to improve cowpea (Vigna unguiculataL. walp.) and green gram (Vigna radiataL. wilczek.) yields in Kenya

Potential of indigenous bradyrhizobia versus commercial inoculants to improve cowpea (Vigna unguiculataL. walp.) and green gram (Vigna radiataL. wilczek.) yields in Kenya

Nodulação e eficiência da fixação do N2 em feijão-caupi por efeito da taxa do inóculo

Selecting improved Lotus nodulating rhizobia to expedite the development of new forage species

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