Mannose-binding lectin and its roles in immune responses in grass carp (Ctenopharyngodon idella) against Aeromonas hydrophila

Dang, Yunfei; Meng, Xin-Zhan; Wang, Shentong; Li, Lisen; Zhang, Meng; Hu, Moyan; Xu, Xiaoyan; Shen, Yubang; Lv, Liqun; Wang, Rongquan; Li, Jiale

doi:10.1016/j.fsi.2017.11.013

Cited by 26 publications

(6 citation statements)

References 46 publications

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“…In the process of previous research on the innate immunity of fish, the liver and spleen of teleost fishes were used as the key research objects (Dang et al ., 2016, 2017, 2018a, 2018b), and in previous studies on the innate immune response of yellow catfish, the liver and spleen were also the focus of research (Cheng et al ., 2020; Tang et al ., 2021). To study the role of trafs in the immune response of yellow catfish, the expression patterns of traf1‐7 in the liver and spleen under A. hydrophila infection were analysed.…”

Section: Resultsmentioning

confidence: 99%

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Genome‐wide identification of the traf gene family in yellow catfish (Pelteobagrus fulvidraco) and analysis of their expression in response to bacterial challenge

Nie

Wang

Zhang

et al. 2022

Journal of Fish Biology

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Tumour necrosis factor (TNF) receptor‐associated factor (TRAF) is a receptor protein that has important functions in the immune system. Nonetheless, there have been few reports of traf genes in teleost fishes. The present study aimed to identify the traf genes from the genomic information of yellow catfish (Pelteobagrus fulvidraco). Eight traf genes were identified and named, which are distributed on different chromosomes but have similar conserved protein domains. Phylogenetic and syntenic analyses demonstrated conservation of traf genes during evolution. In addition, yellow catfish has the relatively rare traf1 and traf5 genes. Gene structure and motif analysis revealed the homology and distribution diversity of the traf genes. Quantitative real‐time reverse transcription PCR was used to study the expression patterns of traf genes in healthy fish tissues and after infection by Aeromonas hydrophila. The results demonstrated significant changes in traf gene expression, indicating a potential role in innate immunity.

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Section: Resultsmentioning

confidence: 99%

“…The source of A. hydrophila is the same as in the previous study (Dang et al ., 2016, 2017, 2018a, 2018b). A. hydrophila was separated and purified, and then a single colony was inoculated into a 1.5 ml EP tube containing Luria‐Bertani (LB) liquid medium and cultured on a shaker at 28 °C.…”

Section: Methodsmentioning

confidence: 99%

Genome‐wide identification of the traf gene family in yellow catfish (Pelteobagrus fulvidraco) and analysis of their expression in response to bacterial challenge

Nie

Wang

Zhang

et al. 2022

Journal of Fish Biology

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“…MBL is activated by binding to mannose residues and several other carbohydrates that are part of the cell wall of a number of pathogens. This reaction activates MBL-associated serine proteases that cleave and thereby activate complement components involved in pathogen lysis ( Nakao et al, 2006 ; Dang et al, 2018 ). In addition, the enzyme peptidyl-prolyl cis-trans isomerase (PPIase) was identified only in the plasma of males collected from the sea.…”

Section: Discussionmentioning

confidence: 99%

Proteins journey—from marine to freshwater ecosystem: blood plasma proteomic profiles of pink salmon Oncorhynchus gorbuscha Walbaum, 1792 during spawning migration

2023

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The pink salmon (Oncorhynchus gorbuscha) is a commercial anadromous fish species of the family Salmonidae. This species has a 2-year life cycle that distinguishes it from other salmonids. It includes the spawning migration from marine to freshwater environments, accompanied by significant physiological and biochemical adaptive changes in the body. This study reveals and describes variability in the blood plasma proteomes of female and male pink salmon collected from three biotopes—marine, estuarine and riverine—that the fish pass through in spawning migration. Identification and comparative analysis of blood plasma protein profiles were performed using proteomics and bioinformatic approaches. The blood proteomes of female and male spawners collected from different biotopes were qualitatively and quantitatively distinguished. Females differed primarily in proteins associated with reproductive system development (certain vitellogenin and choriogenin), lipid transport (fatty acid binding protein) and energy production (fructose 1,6-bisphosphatase), and males in proteins involved in blood coagulation (fibrinogen), immune response (lectins) and reproductive processes (vitellogenin). Differentially expressed sex-specific proteins were implicated in proteolysis (aminopeptidases), platelet activation (β- and γ-chain fibrinogen), cell growth and differentiation (a protein containing the TGF_BETA_2 domain) and lipid transport processes (vitellogenin and apolipoprotein). The results are of both fundamental and practical importance, adding to existing knowledge of the biochemical adaptations to spawning of pink salmon, a representative of economically important migratory fish species.

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“…Referring to the antimicrobial peptide database (APD3, http://aps. unmc.edu/AP/main.php) and published articles on antimicrobial peptides in fish, we focused on 25 antimicrobial peptide families: thrombin, histone, galectin, c-type lectin, collectin, ubiquitin, cyclin-dependent protein kinases (CDK-like), bovine pancreatic trypsin inhibitor (BPTI), chemokine, neuropeptide, phospholipase A2 (PLA2), transferrin, hemoglobin, defensin, actinoporin, liverexpressed antimicrobial peptide 2 (LEAP-2), beta2-microglobulin, thymosin, amyloid, enhancers of rudimentary (ERH), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), saposin, hepcidin, mannose-binding lectin and lysozyme (47)(48)(49)(50)(51). The lined seahorse genome database was downloaded from PRJNA347499 (46), and the homologous genes of antimicrobial peptides were searched by gene names mentioned above in the annotation file, and ascertained by BLAST (e-value = 1 × 10 −5 ) based on amino acid sequences in cds file.…”

Section: Molecular Evolution Of Antimicrobial Peptidesmentioning

confidence: 99%

Hepcidin Gene Co-Option Balancing Paternal Immune Protection and Male Pregnancy

et al. 2022

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Viviparity has originated independently more than 150 times in vertebrates, while the male pregnancy only emerged in Syngnathidae fishes, such as seahorses. The typical male pregnancy seahorses have closed sophisticated brood pouch that act as both uterus and placenta, representing an excellent model system for studying the evolutionary process of paternal immune protection. Phylogenetic analysis indicated that the hampII gene family has multiple tandem duplicated genes and shows independent lineage-specific expansion in seahorses, and they had the highest ratio of nonsynonymous substitutions to synonymous substitutions (dN/dS) in the seahorse phylogenetic branch. The expression levels of hampIIs in the brood pouch placenta were significantly higher during pregnancy than non-pregnancy. Both LPS stimulation test in vivo and cytotoxicity test in vitro proved the immunological protection function of hampIIs against pathogen infection in seahorse. Besides, seahorse hampII peptides exhibit weaker antibacterial function, but stronger agglutination and free endotoxin inhibition. We assumed that the modified immunological function seemed to be a trade-off between the resistance to microbial attack and offspring protection. In brief, this study suggests that the rapid co-option of hampIIs contributes to the evolutionary adaption to paternal immune care during male pregnancy.

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Mannose-binding lectin and its roles in immune responses in grass carp (Ctenopharyngodon idella) against Aeromonas hydrophila

Cited by 26 publications

References 46 publications

Genome‐wide identification of the traf gene family in yellow catfish (Pelteobagrus fulvidraco) and analysis of their expression in response to bacterial challenge

Genome‐wide identification of the traf gene family in yellow catfish (Pelteobagrus fulvidraco) and analysis of their expression in response to bacterial challenge

Proteins journey—from marine to freshwater ecosystem: blood plasma proteomic profiles of pink salmon Oncorhynchus gorbuscha Walbaum, 1792 during spawning migration

Hepcidin Gene Co-Option Balancing Paternal Immune Protection and Male Pregnancy

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