Mannose-binding lectin haplotypes influence Brucella abortus infection in the water buffalo (Bubalus bubalis)

Abstract

A case-control study established that the haplotype pair HYA/HYA at the MBL (mannose binding lectin) locus of water buffalo is associated with resistance to Brucella abortus infection (P < 10(-7)) and the haplotype pairs LYD/LYD with susceptibility to the same pathogen (P < 10(-7)). The subjects included in the present study were tested twice-at a 1-month interval-for the presence of anti-B. abortus antibodies in the serum by agglutination, complement fixation and flow cytometry. Cases (335 subjects) included animals consistently positive to all these tests; controls (335 subjects) comprised animals exposed yet negative by the same tests. The serum from genetically resistant subjects displayed in vitro significantly higher antibacterial activity compared to the serum from genetically susceptible subjects, lending biological significance to the results from the association study. Inhibition of the antibacterial activity following heat treatment of the serum, addition of specific MBL inhibitors (EDTA, mannose, N-acetyl-D: -glucosamine) or anti-human MBL antiserum provide convincing evidence that the antibacterial activity present in the serum results from the interaction between MBL and B. abortus. A replication study (comprising 100 cases and 100 controls) confirmed the results from the original study.

Association study of genetic variants at single nucleotide polymorphism rs109231409 of mannose-binding lectins 1 gene with mastitis susceptibility in Vrindavani crossbred cattle

“…SNPs of MBL1 gene in various breeds of pigs were found to be associated with impaired disease resistance [25]. Both MBL1and MBL2 gene mutations were found to be having impact on susceptibility to different infections [26]. Variations in the coding and the non-coding regions of the MBL gene in various species resulted in innate immune dysfunction [23].…”

Association of novel single nucleotide polymorphisms of the CXCR1 gene with the milk performance traits of Chinese native cattle

“…Compared with SNPs, haplotypes are likely to exert a greater effect on traits (Capparelli et al, 2008). In addition, haplotype analysis is more comprehensive than single-locus analysis (Fallin et al, 2001).…”

Novel SNPs of the mannan-binding lectin 2 gene and their association with production traits in Chinese Holsteins

“…Variations of both MBL1 and MBL2 have been associated with resistance to infections such as S. aureus in mice (Shi et al, 2004), K88+ E. coli, Streptococcus suis, Actinobacillus pleuropneumoniae, Mycoplasma spp, and circovirus 2 in pigs (Lillie et al, 2007), B. abortus in water buffalo (Capparelli et al, 2008), Neorickettsia risticii and Clostridium spp in horses (Podolsky et al, 2006), and meningococcal and E. coli infections in humans (Neth et al, 2000;Eisen and Minchinton, 2003;Smithson et al, 2007). Because bacteria are the most common causes of mastitis in cows, analysis of bovine MBL2 SNPs may contribute to the discovery of their association with mastitis resistance.…”

“…Impaired disease resistance has been associated with 3 single-nucleotide polymorphisms (SNPs) within the coding region of MBL1 in various breeds of pigs (Lillie et al, 2006). Studies of human MBL-C have revealed that 3 SNPs in the collagen-like domain and 3 additional mutations within the MBL2 promoter region can affect the assembly or formation of MBL-C, thus contributing to a low level of plasma MBL and innate immune dysfunction (Madsen et al, 1994(Madsen et al, , 1998Capparelli et al, 2008). Accumulating data have revealed that haplotype combinations of human MBL2 SNPs generate increasing susceptibility to various bacterial, viral, and parasitic diseases as well as to systemic lupus erythematosus, rheumatoid arthritis, hepatitis, cystic fibrosis, and ischemiareperfusion injury (Eisen and Minchinton, 2003;Holmskov et al, 2003;Takahashi et al, Genetics and Molecular Research 11 (4): 3744-3754 (2012) Z.L.…”

“…Zhao et al 2005). A case-control study has demonstrated that polymorphism at the MBL2 locus of water buffalo is associated with susceptibility and resistance to Brucella abortus infection (Capparelli et al, 2008).…”