Manometric, Titrimetric, and Colorimetric Methods for Measurement of Urease Activity

Slyke, Donald D. Van; Archibald, Reginald M.

doi:10.1016/s0021-9258(18)71897-8

Cited by 125 publications

(16 citation statements)

References 15 publications

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“…The activity of urease has been measured by the release of ammonia and carbon dioxide from urea over fixed intervals of time. The techniques include Nesslerization (Sumner, 1926;Kistiakowsky et al, 1952), titration of the ammonia (Van Slyke and Cullen, 1914; Sumner and Hand, 1928;Gorin et al, 1962;Gorin and Chin, 1966), and manometric determination of carbon dioxide (Van Slyke, 1927;Van Slyke and Archibald, 1944). In addition, color reactions are available, at least in principle (Momose et al, 1965).…”

mentioning

confidence: 99%

Jack bean urease (EC 3.5.1.5). A new purification and reliable rate assay

Blakeley¹,

Webb²,

Zerner³

1969

Biochemistry

120

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mentioning

confidence: 99%

Jack bean urease (EC 3.5.1.5). A new purification and reliable rate assay

Blakeley¹,

Webb²,

Zerner³

1969

Biochemistry

120

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“…Kojic acid was used as a standard tyrosinaseinhibitor. Urease inhibitory activity was determined according to Van Slyke and Archibald (1944). Thiourea used as standard urease inhibitor agent.…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial, antioxidant, anti-tyrosinase and anti-urease activities and FT-IR analyses of Helichrysum graveolens (Bieb) Sweet

Aydın¹

2019

Bangladesh J. Bot.

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Helichrysum graveolens (Bieb) Sweet possesses various medicinal applications such as diuretic, treatment of the sand and stone in kidney, regulation of digestion problems and strenghtening of the immune system. The investigation was carried out to reveal ethanol extracts of flower and stem parts of H. graveolens for antimicrobial, antioxidant, anti-tyrosinase and anti-urease activities. Ethanol extract of stem part of H. graveolens exhibited more antibacterial activity against growth of bacteria except for K. pneumoniae. Stem part of H. graveolens has higher copper reducing antioxidant capacity (CUPRAC) and anti-urease activities than flower part of H. graveolens. Anti-tyrosinase activity (IC50 values) of stem and flower parts of H. graveolens was found to be 0.01549 ± 0.0006 μg/ml and 0.01515 ± 0.0016 μg/ml, respectively. This survey suggests that ethanol extracts of different parts of H. graveolens is an alternative source for natural antioxidant and antimicrobial compounds, urease and tyrosinase inhibitors that could be utilized in drug and food industries.

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“…Urease inhibitory activity was assayed by the spectrophotometric method developed by Van Slyke and Archibald (19), in which urea is hydrolyzed over time to yield ammonia and carbon dioxide. Briefly, an assay mixture of a total volume of 1.9 mL, containing extract solution (or standard inhibitor), phosphate buffer (100 mM, pH 6.8 and containing 500 mM urea), and urease solution (16 mg/mL) was pre-incubated at room temperature for 15 min.…”

Section: In Vitro Urease Inhibitory Activitymentioning

confidence: 99%

Histone Deacetylase, Xanthine Oxidase and Urease Inhibitory Activities of Eremurus spectabilis M. Bieb. Extracts

Bayrak¹,

Yanardağ²

2021

experimed

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Objective: Natural ingredients derived from plants (especially edible ones) continue to be investigated for understanding whether they have therapeutic/preventive effects on certain ailments, such as cancer and gastrointestinal disorders. The main goal of the current work is to investigate the inhibitory potential of Eremurus spectabilis M. Bieb. extracts on histone deacetylase (HDAC), xanthine oxidase (XO) and urease activities.Material and Method: Fresh E. spectabilis leaves were obtained from a local market in Eyup/Istanbul. Inhibitory activities of ethanolic and ethyl acetate extracts on HDAC, XO and urease were examined.Results: According to our findings, ethanolic and ethyl acetate extracts of E. spectabili both have revealed apparent inhibitory effects against all examined enzymes. Ethanolic extract showed a better inhibitory effect on HDAC than ethyl acetate extract. On the other hand, the inhibitory effects of ethyl acetate extract against both XO and urease were higher than the ethanolic extract. Conclusion:Consequently, due to the inhibitory effects of the plant being studied, it might be suggested that it can be used for medicinal purposes, in raw extract form, or as a source of bioactive compounds.

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Manometric, Titrimetric, and Colorimetric Methods for Measurement of Urease Activity

Cited by 125 publications

References 15 publications

Jack bean urease (EC 3.5.1.5). A new purification and reliable rate assay

Jack bean urease (EC 3.5.1.5). A new purification and reliable rate assay

Antimicrobial, antioxidant, anti-tyrosinase and anti-urease activities and FT-IR analyses of Helichrysum graveolens (Bieb) Sweet

Histone Deacetylase, Xanthine Oxidase and Urease Inhibitory Activities of Eremurus spectabilis M. Bieb. Extracts

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