Manufacturing and Characterization of 3‐D Hydroxyapatite Bone Tissue Engineering Scaffolds

Chu, Tien Min G.; Hollister, Scott J.; Halloran, John W.; Feinberg, S. E.; Orton, David G.

doi:10.1111/j.1749-6632.2002.tb03061.x

Cited by 54 publications

(37 citation statements)

References 8 publications

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“…Hydroxyapatite (HA) has also attracted a great deal of attention recently [12], [13]. HA has been widely used in medicine because it is osteoconductive and has excellent biological affinity with bony tissue [14], possessing a similar chemical composition and structure as the mineral phase of bones.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Healing of Rat Calvarial Defects with MSCs Loaded on BMP-2 Releasing Chitosan/Alginate/Hydroxyapatite Scaffolds

Liu

Yuan

et al. 2014

PLoS ONE

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In this study, we designed a chitosan/alginate/hydroxyapatite scaffold as a carrier for recombinant BMP-2 (CAH/B2), and evaluated the release kinetics of BMP-2. We evaluated the effect of the CAH/B2 scaffold on the viability and differentiation of bone marrow mesenchymal stem cells (MSCs) by scanning electron microscopy, MTS, ALP assay, alizarin-red staining and qRT-PCR. Moreover, MSCs were seeded on scaffolds and used in a 8 mm rat calvarial defect model. New bone formation was assessed by radiology, hematoxylin and eosin staining 12 weeks postoperatively. We found the release kinetics of BMP-2 from the CAH/B2 scaffold were delayed compared with those from collagen gel, which is widely used for BMP-2 delivery. The BMP-2 released from the scaffold increased MSC differentiation and did not show any cytotoxicity. MSCs exhibited greater ALP activity as well as stronger calcium mineral deposition, and the bone-related markers Col1α, osteopontin, and osteocalcin were upregulated. Analysis of in vivo bone formation showed that the CAH/B2 scaffold induced more bone formation than other groups. This study demonstrates that CAH/B2 scaffolds might be useful for delivering osteogenic BMP-2 protein and present a promising bone regeneration strategy.

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Section: Introductionmentioning

confidence: 99%

Enhanced Healing of Rat Calvarial Defects with MSCs Loaded on BMP-2 Releasing Chitosan/Alginate/Hydroxyapatite Scaffolds

Liu

Yuan

et al. 2014

PLoS ONE

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“…Craniofacial-derived stem cells have potential implications in the tissue engineering of not only craniofacial structures, but also non-craniofacial tissues Sonoyama et al, 2005). Tissue-engineered bone with customized shape and dimensions has the potential for the biological replacement of not only craniofacial bones, but also of segmental defects in the appendicular bones (Feinberg et al, 2001;Chu et al, 2002;Moore et al, 2004). Calvarial bone defects are the most frequently used models for bone tissue engineering (Cowan et al, 2004;Meinel et al, 2005).…”

Section: (6) Impact Of Craniofacial Tissue Engineering On Clinical Prmentioning

confidence: 99%

Craniofacial Tissue Engineering by Stem Cells

et al. 2006

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Craniofacial tissue engineering promises the regeneration or de novo formation of dental, oral, and craniofacial structures lost to congenital anomalies, trauma, and diseases. Virtually all craniofacial structures are derivatives of mesenchymal cells. Mesenchymal stem cells are the offspring of mesenchymal cells following asymmetrical division, and reside in various craniofacial structures in the adult. Cells with characteristics of adult stem cells have been isolated from the dental pulp, the deciduous tooth, and the periodontium. Several craniofacial structures-such as the mandibular condyle, calvarial bone, cranial suture, and subcutaneous adipose tissue-have been engineered from mesenchymal stem cells, growth factor, and/or gene therapy approaches. As a departure from the reliance of current clinical practice on durable materials such as amalgam, composites, and metallic alloys, biological therapies utilize mesenchymal stem cells, delivered or internally recruited, to generate craniofacial structures in temporary scaffolding biomaterials. Craniofacial tissue engineering is likely to be realized in the foreseeable future, and represents an opportunity that dentistry cannot afford to miss.

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“…[5][6][7][8] Current technologies for patterning hydrogels have focused on creating features using layer-by-layer stacking (fused with heat, adhesives, or light), [2] molding, [9,10] 3D printing, [11] electrochemical deposition, [12] and photolithography. [5,13] These technologies have proven to be successful in producing intricate patterns within hydrogels but only address applications that require static features present before material implantation or experimentation.…”

mentioning

confidence: 99%