Manufacturing the Next Generation of Highly Sensitive and Reproducible Lateral Flow Immunoassay

Tisone, Thomas C.; O’Farrell, Brendan

doi:10.1007/978-1-59745-240-3_8

Cited by 14 publications

(10 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The clinical utility of the developed test should be further evaluated with a cohort of healthy controls and with samples from patients with chest pain visiting EDs. Furthermore, LFIA strip manufacturing and reporter drying process is known to be critical for the test performance even in industrial scale 35 . Therefore, the final clinical performance of the test, including the determination proper reference ranges of the UCNP-LFIA, should be evaluated with a finally optimized production prototype of the UCNP-LFIA manufactured with controlled and reproducible processes.…”

Section: Discussionmentioning

confidence: 99%

Sensitive and quantitative detection of cardiac troponin I with upconverting nanoparticle lateral flow test with minimized interference

Bayoumy

Martiskainen

Heikkilä

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Measurement of cardiac troponin I (cTnI) should be feasible for point-of-care testing (POCT) to diagnose acute myocardial infarction (AMI). Lateral flow immunoassays (LFIAs) have been long implemented in POCT and clinical settings. However, sensitivity, matrix effect and quantitation in lateral flow immunoassays (LFIAs) have been major limiting factors. The performance of LFIAs can be improved with upconverting nanoparticle (UCNP) reporters. Here we report a new methodological approach to quantify cTnI using UCNP-LFIA technology with minimized plasma interference. The performance of the developed UCNP-LFIA was evaluated using clinical plasma samples (n = 262). The developed UCNP-LFIA was compared to two reference assays, the Siemens Advia Centaur assay and an in-house well-based cTnI assay. By introducing an anti-IgM scrub line and dried EDTA in the LFIA strip, the detection of cTnI in plasma samples was fully recovered. The UCNP-LFIA was able to quantify cTnI concentrations in patient samples within the range of 30–10,000 ng/L. The LoB and LoD of the UCNP-LFIA were 8.4 ng/L and 30 ng/L. The method comparisons showed good correlation (Spearman’s correlation 0.956 and 0.949, p < 0.0001). The developed UCNP-LFIA had LoD suitable for ruling in AMI in patients with elevated cTnI levels and was able to quantify cTnI concentrations in patient samples. The technology has potential to provide simple and rapid assay for POCT in ED setting

show abstract

Section: Discussionmentioning

confidence: 99%

Sensitive and quantitative detection of cardiac troponin I with upconverting nanoparticle lateral flow test with minimized interference

Bayoumy

Martiskainen

Heikkilä

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…When a solution of the conjugates of the target and the secondary antibody (in this case IgG and gold nanoparticle conjugated anti-IgG (aIgG-AuNP)), IgG-aIgG-AuNP, is applied from the side of the NC membrane, the conjugates move to the surface of NC membrane due to capillary forces. In this regard, the process of conjugation and hybridization with the immobilized antibodies is similar to that of a lateral-flow immunoassay [17] and the details about fabrication of the application pad and the conjugate pad can be found in [18] and has been omitted here for the sake of brevity. Due to antibody-antigen hybridization, a sandwich structure (aIgG-IgG-aIgG-AuNP) is formed and is shown in Fig.…”

Section: A Igg Detection: Principle Materials and Methodsmentioning

confidence: 99%

Wireless Biosensing Using Silver-Enhancement Based Self-Assembled Antennas in Passive Radio Frequency Identification (RFID) Tags

et al. 2015

View full text Add to dashboard Cite

In this paper, we present a silver-enhancement technique for self-assembling radio-frequency (RF) antennas and demonstrate its application for remote biosensing. When target analytes or pathogens are present in a sample the silverenhancement process self-assembles a chain of micro-monopole antennas. As the size of the silver-enhanced particles grows, the chain of micro-antenna segments bridge together to complete a macro-antenna structure. The change in the electrical impedance across the bridge modulates the reflection properties of the antenna at a desired frequency. In this paper we have used this principle to model, optimize and design a ratiometric mode 915 MHz radio-frequency identification (RFID) based biosensor which uses relative received signal strength indicator (RSSI) to measure and detect different concentration levels of target analytes. We have validated the proof-of-concept for detecting two types of analytes: (a) IgG in rabbit serum at concentration levels ranging from 20 ng to 60 ng; and (b) moisture in a sample at volumes ranging from 5µl to 40µl. A significant advantage of the proposed biosensor is that the concentration level of target analytes or pathogens can be remotely interrogated in a concealed, packaged or in a bio-hazardous environment, where direct electrical or optical measurement is considered to be impractical.

show abstract

“…Similar to the operation principle of the lateralflow immunoassay method [19], the target analyte, IgG in this case, first conjugates with the gold nanoparticle labeled antiIgG to form a partial sandwich structure (IgG-aIgG-AuNP). When the partial sandwich conjugate is applied to the AuNP Pad, it flows along the nitrocellulose membrane due to its capillary force.…”

Section: B Integration With Paper-based Microfluidicsmentioning

confidence: 98%

Self-Powered Wireless Affinity-Based Biosensor Based on Integration of Paper-Based Microfluidics and Self-Assembled RFID Antennas

Yuan

Alocilja

Chakrabartty

2016

IEEE Trans. Biomed. Circuits Syst.

View full text Add to dashboard Cite

This paper presents a wireless, self-powered, affinity-based biosensor based on the integration of paper-based microfluidics with our previously reported method for self-assembling radio-frequency (RF) antennas. At the core of the proposed approach is a silver-enhancement technique that grows portions of a RF antenna in regions where target antigens hybridize with target specific affinity probes. The hybridization regions are defined by a network of nitrocellulose based microfluidic channels which implement a self-powered approach to sample the reagent and control its flow and mixing. The integration substrate for the biosensor has been constructed using polyethylene and the patterning of the antenna on the substrate has been achieved using a low-cost ink-jet printing technique. The substrate has been integrated with passive radio-frequency identification (RFID) tags to demonstrate that the resulting sensor-tag can be used for continuous monitoring in a food supply-chain where direct measurement of analytes is typically considered to be impractical. We validate the proof-of-concept operation of the proposed sensor-tag using IgG as a model analyte and using a 915 MHz Ultra-high-frequency (UHF) RFID tagging technology.

show abstract

Manufacturing the Next Generation of Highly Sensitive and Reproducible Lateral Flow Immunoassay

Cited by 14 publications

References 0 publications

Sensitive and quantitative detection of cardiac troponin I with upconverting nanoparticle lateral flow test with minimized interference

Sensitive and quantitative detection of cardiac troponin I with upconverting nanoparticle lateral flow test with minimized interference

Wireless Biosensing Using Silver-Enhancement Based Self-Assembled Antennas in Passive Radio Frequency Identification (RFID) Tags

Self-Powered Wireless Affinity-Based Biosensor Based on Integration of Paper-Based Microfluidics and Self-Assembled RFID Antennas

Contact Info

Product

Resources

About