Maple Syrup: Chemical Analysis and Nutritional Profile, Health Impacts, Safety and Quality Control, and Food Industry Applications

Saraiva, Ariana; Carrascosa, Conrado; Ramos, Fernando; Raheem, Dele; Lopes, María Celeste; Raposo, António

doi:10.3390/ijerph192013684

Cited by 13 publications

(9 citation statements)

References 85 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Obtaining brazzein or monellin from the native plants that produce them (Pentadiplandra brazzeana and Dioscoreophyllum cumminsii, respectively) has not proven to be commercially viable and so there is considerable interest in their heterologous production in either established crop plants or in microorganisms amenable to industrial fermentation. 34 Single-chain monellin has greater thermal stability compared to the native protein consisting of two polypeptide chains. Sc-monellin was successfully secreted in S. cerevisiae, utilizing the MFα1 prepro-SP under the control of a GAL1 promoter.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

A Yeast Modular Cloning (MoClo) Toolkit Expansion for Optimization of Heterologous Protein Secretion and Surface Display in Saccharomyces cerevisiae

O’Riordan,

Jurić,

O’Neill

et al. 2024

ACS Synth. Biol.

View full text Add to dashboard Cite

Saccharomyces cerevisiae is an attractive host for the expression of secreted proteins in a biotechnology context. Unfortunately, many heterologous proteins fail to enter, or efficiently progress through, the secretory pathway, resulting in poor yields. Similarly, yeast surface display has become a widely used technique in protein engineering but achieving sufficient levels of surface expression of recombinant proteins is often challenging. Signal peptides (SPs) and translational fusion partners (TFPs) can be used to direct heterologous proteins through the yeast secretory pathway, however, selection of the optimal secretion promoting sequence is largely a process of trial and error. The yeast modular cloning (MoClo) toolkit utilizes type IIS restriction enzymes to facilitate an efficient assembly of expression vectors from standardized parts. We have expanded this toolkit to enable the efficient incorporation of a panel of 16 well-characterized SPs and TFPs and five surface display anchor proteins into S. cerevisiae expression cassettes. The secretion promoting signals are validated by using five different proteins of interest. Comparison of intracellular and secreted protein levels reveals the optimal secretion promoting sequence for each individual protein. Large, protein of interestspecific variations in secretion efficiency are observed. SP sequences are also used with the five surface display anchors, and the combination of SP and anchor protein proves critical for efficient surface display. These observations highlight the value of the described panel of MoClo compatible parts to allow facile screening of SPs and TFPs and anchor proteins for optimal secretion and/ or surface display of a given protein of interest in S. cerevisiae.

show abstract

Section: ■ Results and Discussionmentioning

confidence: 99%

A Yeast Modular Cloning (MoClo) Toolkit Expansion for Optimization of Heterologous Protein Secretion and Surface Display in Saccharomyces cerevisiae

O’Riordan,

Jurić,

O’Neill

et al. 2024

ACS Synth. Biol.

View full text Add to dashboard Cite

show abstract

“…Because NTG is partially soluble in water, we surmised that it may be present in maple xylem sap and in maple syrup, which is derived from the sap via boiling using open pan heating, sometimes combined with reverse osmosis ( Saraiva et al, 2022 ). To test the antibiofilm activity of maple syrup, we purchased maple syrup from manufacturers in Vermont and New York, the two largest maple syrup producer states in the USA ( USDA, 2023 ).…”

Section: Resultsmentioning

confidence: 99%

Lignan-containing maple products inhibit Listeria monocytogenes biofilms on fresh produce

Elbakush,

Fulano,

Gomelsky

2023

Front. Microbiol.

View full text Add to dashboard Cite

Major listeriosis outbreaks have been associated with fresh produce contaminated with Listeria monocytogenes. Strains that synthesize the Pss exopolysaccharide (EPS) have an estimated 102 to 104-fold advantage over nonsynthesizing strains in causing listeriosis. They more readily attach to the surfaces of fruit and vegetables forming EPS-biofilms that better withstand stresses associated with produce storage and consumption. Here, we show that the threat to fresh produce safety posed by the listerial EPS-biofilms may be countered by broadly available maple products. We serendipitously discovered that aqueous extracts of wood from several Acer (maple) and Carya (pecan, hickory) species inhibit the formation of listerial EPS-biofilms without affecting bacterial viability. One active ingredient in maple wood was identified as nortrachelogenin-8’-O-β-D-glucopyranoside (NTG). At 120 μM, this lignan decreased colonization of the EPS-synthesizing L. monocytogenes on cantaloupe pieces by approximately 150-fold, and on cut celery and lettuce by 10 to 11-fold. Another lignan, lariciresinol, which is abundant in a common food sweetener, maple syrup, had antibiofilm activity comparable to that of NTG. Diluted in the range of 1:200 to 1:800 maple syrup from two random manufacturers prevented formation of listeiral EPS-biofilms. Importantly, not only did maple products drastically decrease colonization of fresh produce by the EPS-synthesizing strains, they also decreased, by 6 to 30-fold, colonization by the L. monocytogenes strains that do not synthesize measurable EPS, including strains from the infamous 2011 cantaloupe listeriosis outbreak. Inhibition of surface colonization by various listerial strains, broad availability of maple sap and syrup as well as maple lumber processing waste position maple products as potential antibiofilm agents for protecting fresh produce from L. monocytogenes.

show abstract

“…Sweet proteins have potential applications as low-calorie sweeteners. Obtaining brazzein or monellin from the native plants that produce them (Pentadiplandra brazzeana and Dioscoreophyllum cumminsii respectively) has not proven to be commercially viable and so there is considerable interest in their heterologous production in either established crop plants or in microorganisms amenable to industrial fermentation (29). Single-chain monellin has greater thermal stability compared to the native protein consisting of two polypeptide chains.…”

Section: Application Of the Toolkit Expansion For Secretion Of Sweet ...mentioning

confidence: 99%

A yeast modular cloning (MoClo) toolkit expansion for optimization of heterologous protein secretion and surface display inSaccharomyces cerevisiae

O’Riordan,

Jurić,

O’Neill

et al. 2023

Preprint

View full text Add to dashboard Cite

Saccharomyces cerevisiaeis an attractive host for expression of secreted proteins in a biotechnology context. Unfortunately, many heterologous proteins fail to enter, or efficiently progress through, the secretory pathway, resulting in poor yields. Similarly, yeast surface display has become a widely used technique in protein engineering but achieving sufficient levels of surface expression of recombinant proteins is often challenging. Signal peptides (SPs) and translational fusion partners (TFPs) can be used to direct heterologous proteins through the yeast secretory pathway, however, selection of the optimal secretion promoting sequence is largely a process of trial and error. The yeast modular cloning (MoClo) toolkit utilises Type IIS restriction enzymes to facilitate efficient assembly of expression vectors from standardized parts. We have expanded this toolkit to enable the efficient incorporation of a panel of sixteen well-characterized SPs and TFPs and five surface display anchor proteins intoS. cerevisiaeexpression cassettes. The secretion promoting signals were validated using five different proteins of interest. Comparison of intracellular and secreted protein levels revealed the optimal secretion promoting sequence for each individual protein. Large, protein of interest-specific variations in secretion efficiency were observed. SP sequences were also used with the five surface display anchors and the combination of SP and anchor protein proved critical for efficient surface display. These observations highlight the value of the described panel of MoClo compatible parts to allow facile screening of SPs, TFPs and anchor proteins for optimal secretion and/or surface display of a given protein of interest inS. cerevisiae.GRAPHICAL ABSTRACT

show abstract

Maple Syrup: Chemical Analysis and Nutritional Profile, Health Impacts, Safety and Quality Control, and Food Industry Applications

Cited by 13 publications

References 85 publications

A Yeast Modular Cloning (MoClo) Toolkit Expansion for Optimization of Heterologous Protein Secretion and Surface Display in Saccharomyces cerevisiae

A Yeast Modular Cloning (MoClo) Toolkit Expansion for Optimization of Heterologous Protein Secretion and Surface Display in Saccharomyces cerevisiae

Lignan-containing maple products inhibit Listeria monocytogenes biofilms on fresh produce

A yeast modular cloning (MoClo) toolkit expansion for optimization of heterologous protein secretion and surface display inSaccharomyces cerevisiae

Contact Info

Product

Resources

About