Mapping a New Source of Self-fertility in Perennial Ryegrass (<i>Lolium perenne</i> L.)

Aguirre, Andrea Arias; Studer, Bruno; Canto, Javier Do; Frei, Ursula; Lübberstedt, Thomas

doi:10.9787/pbb.2013.1.4.385

Cited by 11 publications

(18 citation statements)

References 20 publications

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“…The fully compatible F 2 bred true in the F 3 while segregation occurred in the 50 % compatibility class (Thorogood and Hayward, 1991). This segregation pattern is consistent with other studies (Arias-Aguirre et al, 2013;Manzanares, 2013;Thorogood et al, 2005) and is in agreement with a single gene action with gamethophytic control, where only the pollen carrying a SF allele can accomplish pollination (Figure 1).…”

Section: Genetic Self-fertilitysupporting

confidence: 91%

“…This distortion could potentially be due to the presence of another SF locus, since some markers on LG3 were found to interact with the S and Z SI loci with alleles co-segregating or even contributing to a SF reaction (Thorogood et al, 2002;Thorogood and Hayward, 1992). However, this region could not be associated with the observed SF segregation and thus, has so far been disregarded as a potential SF locus (Arias-Aguirre et al, 2013;Manzanares, 2013;Thorogood et al, 2002).…”

Section: Origin Of S and Z Sf Allelesmentioning

confidence: 99%

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Overcoming self-incompatibility in grasses: a pathway to hybrid breeding

2016

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Allogamous grasses exhibit an effective two-locus gametophytic self-incompatibility (SI) system, limiting the range of breeding techniques applicable for cultivar development. Current breeding methods based on populations are characterized by comparably low genetic gains for important traits such as biomass yield. To implement more efficient breeding schemes, the overall understanding of the SI system is crucial as are the mechanisms involved in the breakdown of SI. Self-fertile variants in outcrossing grasses have been studied, and the current level of knowledge includes approximate gene locations, linked molecular markers and first hypotheses on their mode of action. Environmental conditions increasing seed set upon self-pollination have also been described. Even though some strategies were proposed to take advantage of self-fertility, there have, so far, not been changes in the methods applied in cultivar development for allogamous grasses. In this review, we describe the current knowledge about self-fertility in allogamous grasses and outline strategies to incorporate this trait for implementation in synthetic and hybrid breeding schemes.

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Section: Genetic Self-fertilitysupporting

confidence: 91%

Section: Origin Of S and Z Sf Allelesmentioning

confidence: 99%

Overcoming self-incompatibility in grasses: a pathway to hybrid breeding

2016

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“…In addition to the loci revealed in this GWAS, a mapped locus (or loci) on LG 5, for which the only variant that has been revealed is one for self-fertility, has been identified in perennial ryegrass (Thorogood et al, 2005 ; Arias Aguirre et al, 2013 ) and in rye (Fuong et al, 1993 ).…”

Section: Discussionmentioning

confidence: 99%

“…The gametophytically controlled reaction of the pollen at the stigma surface enables quantification of the degree of incompatibility between two plants simply by observation of the proportion of compatible and incompatible pollen grains alighting on a stigma surface in so-called semi- in-vivo pollinations. Semi- in-vivo pollination tests have been used successfully for genetic linkage mapping of SI and self-fertility loci in perennial ryegrass (Thorogood et al, 2002 , 2005 ; Arias Aguirre et al, 2013 ). Classically, genetic linkage mapping in outcrossing species is based on segregating populations derived from bi-parental crosses between parents with contrasting phenotypes.…”

Section: Introductionmentioning

confidence: 99%

A Novel Multivariate Approach to Phenotyping and Association Mapping of Multi-Locus Gametophytic Self-Incompatibility Reveals S, Z, and Other Loci in a Perennial Ryegrass (Poaceae) Population

et al. 2017

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Self-incompatibility (SI) is a mechanism that many flowering plants employ to prevent fertilisation by self- and self-like pollen ensuring heterozygosity and hybrid vigour. Although a number of single locus mechanisms have been characterised in detail, no multi-locus systems have been fully elucidated. Historically, examples of the genetic analysis of multi-locus SI, to make analysis tractable, are either made on the progeny of bi-parental crosses, where the number of alleles at each locus is restricted, or on crosses prepared in such a way that only one of the SI loci segregates. Perennial ryegrass (Lolium perenne L.) possesses a well-documented two locus (S and Z) gametophytic incompatibility system. A more universal, realistic proof of principle study was conducted in a perennial ryegrass population in which allelic and non-allelic diversity was not artificially restricted. A complex pattern of pollinations from a diallel cross was revealed which could not possibly be interpreted easily per se, even with an already established genetic model. Instead, pollination scores were distilled into principal component scores described as Compatibility Components (CC1-CC3). These were then subjected to a conventional genome-wide association analysis. CC1 associated with markers on linkage groups (LGs) 1, 2, 3, and 6, CC2 exclusively with markers in a genomic region on LG 2, and CC3 with markers on LG 1. BLAST alignment with the Brachypodium physical map revealed highly significantly associated markers with peak associations with genes adjacent and four genes away from the chromosomal locations of candidate SI genes, S- and Z-DUF247, respectively. Further significant associations were found in a Brachypodium distachyon chromosome 3 region, having shared synteny with Lolium LG 1, suggesting further SI loci linked to S or extensive micro-re-arrangement of the genome between B. distachyon and L. perenne. Significant associations with gene sequences aligning with marker sequences on Lolium LGs 3 and 6 were also identified. We therefore demonstrate the power of a novel association genetics approach to identify the genes controlling multi-locus gametophytic SI systems and to identify novel loci potentially involved in already established SI systems.

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“…Mutations in loci non-allelic to S and Z can also lead to SC, by interrupting the downstream cascade triggered by the initial self-recognition (Do Canto et al 2016). Such putative mutations have been found in rye ( Secale cereale L.) and perennial ryegrass (Voylokov et al 1993; Egorova et al 2000; Thorogood et al 2005; Arias-Aguirre et al 2013, Do Canto et al 2018; Slatter et al 2020). A detailed molecular understanding of how SC arises in SI grasses is lacking, but several studies have acknowledged that calcium (Ca 2+ ) signaling plays an essential role in the recognition and/or inhibition of self-pollen (Yang et al 2009; Chen et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Identification of candidate genes for self-compatibility in perennial ryegrass (Lolium perenneL.)

Cropano

Manzanares

Yates

et al. 2021

Preprint

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Self-incompatibility (SI) is a genetic mechanism preventing self-pollination in approximately 40% of plant species. Two multiallelic loci, called S and Z, control the gametophytic SI system of the grass family (Poaceae), which contains all major forage grasses. Loci independent from S and Z have been reported to disrupt SI and lead to self-compatibility (SC). A locus causing SC in perennial ryegrass (Lolium perenne L.) was previously mapped on linkage group (LG) 5 in a F2 population segregating for SC. Using a subset of the same population (n=73), we first performed low-resolution quantitative trait locus (QTL) mapping to exclude the presence of additional, previously undetected contributors to SC. The previously reported QTL on LG 5 explained 38.4% of the phenotypic variation, and no significant contribution from other genomic regions was found. This was verified by the presence of significantly distorted markers in the region overlapping with the QTL. Second, we fine mapped the QTL to 0.26 cM using additional 2,056 plants and 23 novel sequence-based markers. Using an Italian ryegrass (Lolium multiflorum Lam.) genome assembly as reference, the markers flanking SC were estimated to span a ~3 Mb region encoding for 57 predicted genes. Among these, seven genes were proposed as relevant candidate genes based on their annotation and function described in previous studies. Our work is a step forward to identify SC genes in forage grasses and provides diagnostic markers for marker-assisted introgression of SC into elite germplasm.

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Mapping a New Source of Self-fertility in Perennial Ryegrass (Lolium perenne L.)

Cited by 11 publications

References 20 publications

Overcoming self-incompatibility in grasses: a pathway to hybrid breeding

Overcoming self-incompatibility in grasses: a pathway to hybrid breeding

A Novel Multivariate Approach to Phenotyping and Association Mapping of Multi-Locus Gametophytic Self-Incompatibility Reveals S, Z, and Other Loci in a Perennial Ryegrass (Poaceae) Population

Identification of candidate genes for self-compatibility in perennial ryegrass (Lolium perenneL.)

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