Aerial hyperspectral and multispectral satellite data are the two most commonly used datasets to identify natural and semi-natural vegetation. However, there is no documented analysis based on data from several areas concerning the difference in the classification accuracy of non-forest Natura 2000 habitat with the use of aerial hyperspectral and satellite multispectral data. Also, there is no recommendation, on which habitat can be classified with sufficient accuracy using free multispectral images. This study aimed to analyse the difference in classification accuracy of Natura 2000 habitats representing: meadows, grasslands, heaths and mires between data with different spectral resolutions and the results utility for nature conservation compared to conventional maps. The analysis was conducted in five study areas in Poland. The classification was performed on multispectral Sentinel-2 (S2) and hyperspectral HySpex (HS) images using the Random Forest algorithm. Based on the results, it can be stated that the use of HS data resulted in higher classification accuracy, on average 0.14, than using S2 images, regardless of the area of the habitat. However, the difference in accuracy was not constant, varying by area and habitat characterisation. Greater differences in accuracy were observed for areas where habitats were characterised by high α-diversity or β-diversity. The HS and S2 data make it possible to create maps that provide a great deal of new knowledge about the distribution of Natura 2000 habitats, which is necessary for the management of protected areas. The obtained results indicate that by using S2 images it is possible to identify, at a satisfactory level, alluvial meadows and grassland. For heaths and mires, using HS data improved the results, but it is also possible to acquire general distribution of these classes, whereas HS images are obligatory for mapping salt, Molinia and lowland hay meadows.