Mapping Novel Subcutaneous Angiogenesis Quantitative Trait Loci in [B6×MRL]F2 Mice

Abstract

Objective: MRL/MpJ mice are known for enhanced healing, but mechanistic details or how specific aspects of wounding (e.g., angiogenesis) contribute to healing are unknown. While previous studies investigated the systemic effects of immunity in MRL/MpJ healing, few have focused on tissue-intrinsic effects. Approach: Ex vivo skin biopsies from MRL/MpJ and C57BL/6J mice were cultured in ex vivo conditions that favor endothelial cell growth to compare their angiogenic potential. We localized enhanced angiogenesis quantitative trait loci (QTL) in an F2 intercross. We then performed an expression analysis in cultured skin biopsies from MRL/MpJ and C57BL/6J mice to determine the pathways that are associated with the capacity for differential growth.

Inflammation, Healing, and Genes: A Preface

“…The third study by Morales et al 3 , entitled ''Mapping novel subcutaneous angiogenesis quantitative trait loci in [B6 · MRL]F2 mice,'' focuses on angiogenesis in skin explant cultures. Explants have the obvious benefit of isolating tissue intrinsic effects, but also the less obvious advantage of isolating the target tissue from the mechanical forces of wound contracture imposed upon a punch biopsy flank skin wound, in situ.…”