Mapping of a new gene for resistance to broomrape races higher than F

Imerovski, Ivana; Dimitrijević, Aleksandra; Miladinović, Dragana; Dedić, Boško; Jocić, Siniša; Tubić, Nataša Kočiš; Cvejić, Sandra

doi:10.1007/s10681-015-1597-7

Cited by 27 publications

(24 citation statements)

References 43 publications

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“…Complete monogenic dominant resistance in sunflower has been reported against all the broomrape races described so far, including races A–E (Vranceanu, Tudor, Stoenescu, & Pirvu, 1980), race F (Pacureanu‐Joita, Veronesi, Raranciuc, & Stanciu, 2004), and race G (Velasco, Pérez‐Vich, Yassein, Jan, & Fernández‐Martínez, 2012). Exceptions in which resistance to sunflower broomrape is controlled by other genetic systems, including control by two independent dominant genes (Pacureanu‐Joita, Raranciuc, Stanciu, Sava, & Nastase, 2008), one dominant and one modifying gene (Velasco, Pérez‐Vich, Jan, & Fernández‐Martínez, 2007), one recessive gene (Imerovski et al., 2016), and two recessive genes (Akhtouch, Muñoz‐Ruz, Melero‐Vara, Fernández‐Martínez, & Domínguez, 2002), have also been reported, but even in these cases, resistance was characterized by the complete absence of emerged shoots of the parasite. No studies have investigated the mechanisms underlying the resistance mechanisms in resistant donor genotypes, but the absence of broomrape tubercles is an indicator of either pre‐attachment or pre‐haustorial resistance mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Characterization of post‐haustorial resistance to sunflower broomrape

Martín-Sanz¹,

Pérez‐Vich

Rueda³

et al. 2020

Crop Science

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The development of durable resistance to broomrape (Orobanche cumana Wallr.) in sunflower (Helianthus annuus L.) requires detailed characterization of the genetic and physiological bases of resistance. The objective of the present study was to map the resistance gene accurately, and to characterize the mechanism of resistance to broomrape observed in a sunflower inbred line (PHSC1102). PHSC1102, which was consistently resistant against race F and race G populations of broomrape, was crossed with PHSC1201, which was susceptible to races F and G. A mapping population of 150 F 2 genotypes was phenotyped by evaluating F 2:3 families for resistance to broomrape races F GV and G TK . The use of single nucleotide polymorphism (SNP) markers mapped the Or SII gene to Linkage Group 4 (LG4) of the sunflower genome. Minirhizotron and histological studies of the resistant line revealed that the attachment of broomrape to host roots was similar in both the resistant and susceptible lines and that the resistance was observed at a late stage (i.e., after tubercle development). Interestingly, the resistance of the PHSC1102 line was associated with the production of phenolic compounds, which were hypothesized to restrict the parasite's growth. This research provides novel and valuable information about the host-parasite interactions between sunflower and broomrape.Abbreviations: DAB, 3,3-diaminobenzidine; LG, linkage group; LOD, logarithm of odds; PBS, phosphate-buffered saline; PCR, polymerase chain reaction; QTL, quantitative trait loci; SNP, single nucleotide polymorphism; TBO, toluidine blue O.

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Section: Discussionmentioning

confidence: 99%

Characterization of post‐haustorial resistance to sunflower broomrape

Martín-Sanz¹,

Pérez‐Vich

Rueda³

et al. 2020

Crop Science

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“…For example, one commercial sunflower in Argentina (cultivar M15) showed complete resistance to O. cumana race E [56]. However, it has been pointed out that the majority of the existing resistant genes have become insufficient due to the emergence of race F and higher [57]. Race E avirulence and race F virulence on a sunflower line are allelic and controlled by a single locus [58].…”

Section: Sunflowermentioning

confidence: 99%

“…Race E avirulence and race F virulence on a sunflower line are allelic and controlled by a single locus [58]. To attain sunflower varieties resistant to O. cumana races higher than F, the introduction of resistance genes from wild sunflower species to cultivated sunflowers has been studied [57,[59][60][61][62][63]. An inbred line, AB-VL-8, which originated from a population developed from interspecific hybridization with the rough sunflower (Helianthus divaricatus) was a successful example and had full resistance to races higher than F [57,59].…”

Section: Sunflowermentioning

confidence: 99%

“…To attain sunflower varieties resistant to O. cumana races higher than F, the introduction of resistance genes from wild sunflower species to cultivated sunflowers has been studied [57,[59][60][61][62][63]. An inbred line, AB-VL-8, which originated from a population developed from interspecific hybridization with the rough sunflower (Helianthus divaricatus) was a successful example and had full resistance to races higher than F [57,59]. Successful identification of quantitative trait loci (QTLs) for O. cumana resistance in the sunflower has been reported [63][64][65][66] and utilization of QTLs in the breeding process is expected.…”

Section: Sunflowermentioning

confidence: 99%

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Recent research progress in combatting root parasitic weeds

Samejima

Sugimoto

2018

Biotechnology & Biotechnological Equipment

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The obligate root parasitic Orobanchaceae plants Striga, Orobanche and Phelipanche spp. parasitize economically important crops, vegetables and oil plants. They are the most devastating agricultural weed pests worldwide. Based on an analysis of the climatic requirements of these parasites, very large areas of new territory are at risk of invasion if care is not taken. Recent research in combatting root parasitic weeds was reviewed based on scientific papers reported from 2010 and onwards. The countermeasures fell into eight kinds: resistant varieties, tolerant varieties, microbiological approach, cultural practices, chemical controls, host-induced gene silencing, integrated management and dissemination of technologies including the current situation survey. The development of practical, feasible and economical control technologies against root parasitic weeds would be expected by advancing and combining the countermeasures.

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“…The parasitic interaction between sunflower and O. cumana generally follows a gene for gene model, with resistance in sunflower (Vrânceanu et al, 1980) and avirulence in O. cumana (Rodríguez-Ojeda et al, 2013b) controlled by dominant alleles at single loci. Nonetheless, more complex genetic control of resistance to O. cumana has been also reported in some sunflower resistant sources, including two dominant genes (Domínguez, 1996), one dominant and one recessive gene (Akhtouch et al, 2002; Akhtouch et al, 2016), one dominant and one modifying gene (Velasco et al, 2007), one recessive gene (Imerovski et al, 2016), two recessive genes (Rodríguez-Ojeda et al, 2001; Akhtouch et al, 2002), or polygenic genetic control (Labrousse et al, 2004). …”

Section: Introductionmentioning

confidence: 99%

Increased Virulence in Sunflower Broomrape (Orobanche cumana Wallr.) Populations from Southern Spain Is Associated with Greater Genetic Diversity

Martín-Sanz¹,

Malek

Fernández‐Martínez

et al. 2016

Front. Plant Sci.

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Orobanche cumana Wallr. (sunflower broomrape) is a holoparasitic weed that infects roots of sunflower in large areas of Europe and Asia. Two distant O. cumana gene pools have been identified in Spain, one in Cuenca province in the Center and another one in the Guadalquivir Valley in the South. Race F has been hypothesized to have arisen by separate mutational events in both gene pools. In the Guadalquivir Valley, race F spread in the middle 1990’s to become predominant and contained so far with race F hybrids. Recently, enhanced virulent populations of O. cumana have been observed in commercial fields parasitizing race F resistant hybrids. From them, we collected four independent populations and conducted virulence and SSR marker-based genetic diversity analysis. Virulence essays confirmed that the four populations studied can parasitize most of the race F resistant hybrids tested, but they cannot parasitize the differential inbred lines DEB-2, carrying resistance to race F and G, and P-96, resistant to F but susceptible to races G from other countries. Accordingly, the new populations have been classified as race GGV to distinguish them from other races G. Cluster analysis with a set of populations from the two Spanish gene pools and from other areas, mainly Eastern Europe, confirmed that race GGV populations maintain close genetic relatedness with the Guadalquivir Valley gene pool. This suggested that increased virulence was not caused by new introductions from other countries. Genetic diversity parameters revealed that the four populations had much greater genetic diversity than conventional populations of the same area, containing only alleles present in the Guadalquivir Valley and Cuenca gene pools. The results suggested that increased virulence may have resulted from admixture of populations from the Guadalquivir Valley and Cuenca followed by recombination of avirulence genes.

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Mapping of a new gene for resistance to broomrape races higher than F

Cited by 27 publications

References 43 publications

Characterization of post‐haustorial resistance to sunflower broomrape

Characterization of post‐haustorial resistance to sunflower broomrape

Recent research progress in combatting root parasitic weeds

Increased Virulence in Sunflower Broomrape (Orobanche cumana Wallr.) Populations from Southern Spain Is Associated with Greater Genetic Diversity

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