Mapping of Genetic Determinants of Rubella Virus Associated with Growth in Joint Tissue

Lund, Karen; Chantler, J. K.

doi:10.1128/jvi.74.2.796-804.2000

Cited by 20 publications

(2 citation statements)

References 52 publications

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“…Recently, Lund demonstrated that the growth of rubella virus strains vary in their abilities to replicate and persists in cell cultures derived from human joint tissues, and that this arthrotropism appears to be linked to their association with joint symptoms in vivo (13) .…”

Section: Discussionmentioning

confidence: 99%

Infectivity of wild-type rubella virus in fibrochondrocyte cells

Figueiredo¹,

Oliveira²,

Afonso³

et al. 2004

J. Bras. Patol. Med. Lab.

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This study describes the rapid growth of the rubella virus in samples of a primary fibrochondrocyte cell culture with the development of a cytopathic effect (CPE), in response to infection by the rubella virus. The cells were isolated from the meniscus joint of a rabbit after enzymatic extraction and incubated at 37°C with a Dulbecco's modified Eagle's medium (DMEM), supplemented with 10% fetal calf serum. A total of six clinical samples from urine, blood and cerebrospinal fluid were inoculated in the fibrochondrocyte and the cell lines of the African green monkey kidney -ATCC CCL-81 (Vero). The fibrochondrocyte cell showed CPE after 24 hours and virus growth was confirmed by immunohistochemistry and Nested PCR. The cells inoculated with samples were examined by phase contrast microscopy and showed characteristic rounding, along with bipolar and multipolar cells. The infection curve increased during the five days of observation, showing that the titers in fibrochondrocyte cells were then higher than those observed in Vero cell lines. Fibrochondrocytes Rubella virus Isolation resumoEste trabalho descreve o rápido crescimento do vírus da rubéola em amostras clínicas em cultura primária de fibrocondrócitos com desenvolvimento de efeito citopático em resposta a infecção pelo vírus da rubéola. As células foram isoladas do menisco do joelho do coelho após extração enzimática e incubadas a 37°C em meio DMEM suplementado com 10% de soro fetal bovino. Seis amostras clínicas de urina, sangue e liquor foram inoculadas na cultura primária de fibrocondrócitos e na linhagem Vero. As células de fibrocondrócitos apresentaram efeito citopático após 24 horas de incubação e o vírus foi detectado por imunoistoquímica e Nested PCR. As células infectadas apresentaram aspecto arredondado, com formação de alguns prolongamentos citoplasmáticos e sincícios, produzindo células multinucleadas. A curva de crescimento da infecciosidade do vírus foi mais alta quando comparada com a linhagem celular Vero. Fibrocondrócitos Vírus da rubéola Isolamento unitermos y key words ARTIGO ORIGINAL

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Section: Discussionmentioning

confidence: 99%

Infectivity of wild-type rubella virus in fibrochondrocyte cells

Figueiredo¹,

Oliveira²,

Afonso³

et al. 2004

J. Bras. Patol. Med. Lab.

View full text Add to dashboard Cite

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“…(Lund and Chantler 2000; Mitchell et al 1998) Similarly, rubella vaccine response variability is due, in part, to differences in host genetics. (Jacobson et al 2009; Tan et al 2001) Our lab has identified a number of genes where genetic polymorphisms are associated with vaccine response including: HLA; cytokines and cytokine receptor genes; pattern recognition receptor and antiviral genes; as well as in vitamin receptor and other genes.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide SNP associations with rubella-specific cytokine responses in measles-mumps-rubella vaccine recipients

et al. 2014

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Genetic polymorphisms are known to affect responses to both viral infection and vaccination. Our previous work has described genetic polymorphisms significantly associated with variations in immune response to rubella vaccine from multiple gene families with known immune function, including: HLA, cytokine and cytokine receptor genes, and in genes controlling innate and adaptive immunity. In this study, we assessed cellular immune responses (IFNγ and IL-6) in a cohort of healthy younger individuals and performed genome wide SNP analysis on these same individuals. Here, we report the first genome-wide association study focused on immune responses following rubella vaccination. Our results indicate that rs16928280 in PTPRD (protein tyrosine phosphatase delta) and a collection of SNPs in ACO1 (encoding an iron regulatory protein) are associated with inter-individual variations in IFNγ response to rubella virus stimulation. In contrast, we did not identify any significant genetic associations with rubella-specific IL-6 response. These genetic regions may influence rubella vaccine-induced IFNγ responses and warrant further studies in additional cohorts in order to confirm these findings.

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Genomic analysis of the Chinese genotype 1F rubella virus that disappeared after 2002 in China

et al. 2014

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Genotype 1F was likely localized geographically to China as it has not been reported elsewhere. In this study, whole genome sequences of two rubella 1F virus isolates were completed. Both viruses contained 9,761 nt with a single nucleotide deletion in the intergenic region, compared to the NCBI rubella reference sequence (NC 001545). No evidence of recombination was found between 1F and other rubella viruses. The genetic distance between 1F viruses and 10 other rubella virus genotypes (1a, 1B, 1C, 1D, 1E, 1G, 1J 2A, 2B, and 2C) ranged from 3.9% to 8.6% by pairwise comparison. A region known to be hypervariable in other rubella genotypes was also the most variable region in the 1F genomes. Comparisons to all available rubella virus sequences from GenBank identified 22 nucleotide variations exclusively in 1F viruses. Among these unique variations, C9306U is located within the recommended molecular window for rubella virus genotyping assignment, could be useful to confirm 1F viruses. Using the Bayesian Markov Chain Monte Carlo (MCMC) method, the time of the most recent common ancestor for the genotype 1F was estimated between 1976 and 1995. Recent rubella molecular surveillance suggests that this indigenous strain may have circulated for less than three decades, as it has not been detected since 2002.

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Mapping of Genetic Determinants of Rubella Virus Associated with Growth in Joint Tissue

Cited by 20 publications

References 52 publications

Infectivity of wild-type rubella virus in fibrochondrocyte cells

Infectivity of wild-type rubella virus in fibrochondrocyte cells

Genome-wide SNP associations with rubella-specific cytokine responses in measles-mumps-rubella vaccine recipients

Genomic analysis of the Chinese genotype 1F rubella virus that disappeared after 2002 in China

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