Mapping of quantitative trait loci for partial resistance to Mycosphaerella pinodes in pea (Pisum sativum L.), at the seedling and adult plant stages

Abstract

The inheritance of resistance to Ascochyta blight, an economically important foliar disease of field pea ( Pisum sativum L.) worldwide, was investigated. Breeding resistant pea varieties to this disease, caused by Mycosphaerella pinodes, is difficult due to the availability of only partial resistance. We mapped and characterized quantitative trait loci (QTLs) for resistance to M. pinodes in pea. A population of 135 recombinant inbred lines (RILs), derived from the cross between DP (partially resistant) and JI296 (susceptible), was genotyped with morphological, RAPD, SSR and STS markers. A genetic map was elaborated, comprising 206 markers distributed over eight linkage groups and covering 1,061 cM. The RILs were assessed under growth chamber and field conditions at the seedling and adult plant stages, respectively. Six QTLs were detected at the seedling stage, which together explained up to 74% of the variance. Ten QTLs were identified at the adult plant stage in the field, and together these explained 56.6-67.1% of the variance, depending on the resistance criteria and the organ considered. Four QTLs were detected under both growth chamber and field conditions, suggesting they were not plant-stage dependent. Three QTLs for flowering date and three QTLs for plant height were also identified in the RIL population, some of which co-located with QTLs for resistance. The relationship between QTLs for resistance to M. pinodes, plant height and flowering date is discussed.

Changes in Foliar Host Reaction to Ascochyta Rabiei With Plant Maturity

“…Since resistance is conditioned by multiple quantitative genetic effects (Gowen, Orton, Thurley, & White, 1989;Vail & Banniza, 2008;Flandez-Galves et al, 2003a) with a relatively large environmental influence (Trapero-Casas & Kaiser, 1992), different genes and possibly pathways may be in operation at different growth stages. This has previously been shown for Ascochyta blight of pea (Prioul, Frankewitz, Deniot, Morin, & Baranger, 2004). Selection and integration of the major genetic components of both seedling and maturity resistance would likely increase the durability of resistance and lead to potential for less fungicide applications.…”

Genotypic population structure of Ascochyta pinodes and Phoma pinodella in Poland

“…These stud ies in clude also map ping the ge nome of host or the ge nome of patho gens in order to find QTLs loci con fer ring re sis tance or con fer ring ag gres sive ness or vir u lence and toxin syn the sis (Barranger et al 2006, Bretag et al 1995, Cubero et al 2005, Cho et al 2004, Fondevilla et al 2006, Ford et al 1999, 1991, 1992, Kraft et al 1998, Kraft and Pfleger 2001, Marcinkowska 1996, Marcinkowska and Witkowska 1996, Pilet-Nayel et al 2002, Santra et al 2000, 2001, Prioul et al 2004, Timmerman -Vaughan et al 2000, Wroth 1999, Xue and Warkentin 2001.…”

“…ciceris and Ascochyta rabiei, faba-bean to A.fabae and lentis to A.lentis. Sub stan tial prog ress in the field of un derstand ing the quan ti ta tive re sis tance of pea, chick pea, faba-been and lentis to patho gens has been made (McPhee et al 2006, Cubero et al 2005, Ford et al 1999, Prioul et al 2004, Fondevilla et al 2006.…”

“…Knowl edge on the evo lu tion ary po ten tial of A.pinodes and P.pinodella may prove use ful to op ti mize the man age ment of re sis tance genes, ap plied of fun gi cides, max i mize their use ful and min imize the losses in ef fi cacy of these con trol meth ods. Cur rent re search in terest cover a broad range of sub jects re lated to A.pinodes and P.pinodella bi ol ogy in clud ing: ge net ics and tax on omy at mo lec u lar level, evo lu tion of these patho gens and host-patho gen in ter ac tions, gene flow and ge no type mi gra tion, oc cur rence of sex ual re pro duc tion in Po land and in other countries and phylo gen etic re la tion ships be tween strains in dif fer ent world wide subpopulations of A.pinodes and P.pinodella , Cubero et al 2005, Fatehi 2000, Fondevilla et al 2006, McPhee et al 2006, Peever et al 2002, Prioul et al 2004, Timmerman -Vaughan et al 2000, Wroth 1999, Xue and Warkentin 2001, Zhang et al 2006.…”

Current knowledge on plant/canopy architectural traits that reduce the expression and development of epidemics

“…In some cases, genes responsible for architectural traits and resistance QTLs co-localise. For example, Prioul et al (2004) observed a high relationship between QTLs of pea for resistance to M. pinodes, plant height and flowering date. Similarly, Miklas et al (2003) observed QTLs of resistance to white mold in snap bean associated with canopy height, lodging traits or internode length.…”