Mapping of rat prostatic binding protein genes C1, C2, and C3 to rat chromosome 5 by in situ hybridization

Zhang, Jianjun; Dirckx, Luc; Marynen, Peter; Rombauts, Wilfried; Delaey, Bernard; Berghe, Herman Van den; Cassiman, Jean Jacques

doi:10.1159/000132604

Cited by 11 publications

(5 citation statements)

References 6 publications

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“…In favour of this hypothesis are the loss of similarity of the C2B region with the two other genes upstream of position -150 and the presence of a long purine-pyrimidine stretch at position -615, a simple sequence with potential to form left-handed Z-DNA (Rich, Nordheim & Wang, 1984), which may have been involved in recombinational events (Ann, Smith & Carlson, 1988). Further supporting data come from our local¬ ization of the Cl and C2 genes on chromosome 5 (Zhang, Dirckx, Marynen et al 1988). Indeed, whereas only one Cl-specific band was observed, two C2-specific locations, one of them possibly rep¬ resenting the C2B region, were detected.…”

Section: Discussionsupporting

confidence: 58%

The androgen-dependent rat prostatic binding protein: comparison of the sequences in the 5′ part and upstream region of the Cl and C2 genes and analysis of their transcripts

Claessens

Dirckx²,

Delaey³

et al. 1989

Journal of Molecular Endocrinology

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The complete gene encoding the polypeptide Cl of the complex androgen-controlled prostatic binding protein was isolated from a rat genomic library. A new genomic fragment (C2B) containing only the 5\ m=' \ part of a C2-related gene was also purified. The segments containing exon 1 and a large part of the adjacent sequences were analysed and compared with the corresponding region of the C2A gene which has been completely sequenced previously. The high structural similarity extending over a large part of all three genomic fragments suggests the duplication of a common ancestral gene, followed by a more recent duplication of the C2-coding region. However, since the structural similarity upstream of position \m=-\150 between C2A and C2B abruptly disappears and no transcripts specific for the C2B region can be detected in prostate RNA, we propose that at a later stage in evolution the C2B region was disrupted and inactivated. Despite the common origin and the similar regulation of the two active genes, Cl and C2A, the only obvious conserved structural element is the homopurine stretch located at position \m=-\400, although sequence motifs resembling steroid hormone response elements are present at several locations.

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Section: Discussionsupporting

confidence: 58%

The androgen-dependent rat prostatic binding protein: comparison of the sequences in the 5′ part and upstream region of the Cl and C2 genes and analysis of their transcripts

Claessens

Dirckx²,

Delaey³

et al. 1989

Journal of Molecular Endocrinology

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“…34 The latter assignments should be reinvestigated in the light of our results. Moreover, the rat prostatic binding protein genes C1, C2, and C3 have been mapped to rat chromosome 5q21–31 by in situ hybridization, 67 and the genes for mouse salivary ABP have been shown to be located on chromosome 7 near the glucose phosphate isomerase‐1 locus. 68 Regions of mouse chromosome 7 and human chromosome 11 have been shown already to be syntenic (http://www.informatics.jax.org).…”

Section: Discussionmentioning

confidence: 99%

All Human Genes of the Uteroglobin Family Are Localized on Chromosome 11q12.2 and Form a Dense Cluster

Kalff-Suske

Gentz

et al. 2000

Annals of the New York Academy of Sciences

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Rabbit uteroglobin is the founder member of a family of mammalian proteins that has expanded to more than 20 members within the last few years. All members are small, secretory, rarely glycosylated dimeric proteins with unclear physiological functions and are mainly expressed in mucosal tissues. A phylogenetic analysis shows that the family can be grouped into five subfamilies, A to E. Subfamily A contains rabbit uteroglobin and its orthologues from various species; most of these have been described to form antiparallel homodimers via two intermolecular disulfide bonds. All other subfamily members contain a third conserved cysteine and, from existing biochemical data, it can be predicted that a member of subfamily B or C will likely form heterodimers with a partner from subfamily E or D, respectively. Besides the mentioned cysteines, only one central lysine is conserved in all family members. In the known uteroglobin structures, this lysine forms an exposed salt bridge with an aspartate side chain, which is conserved in almost all sequences. Using radiation hybrid mapping and P1 clone analysis and utilizing data from the human genome project, we show that all known five human family members (Clara cell 10‐kDa protein, lipophilins A and B, lacryglobin, mammaglobin) and a new member, we call lymphoglobin, are localized on chromosome 11q12.2 in a dense cluster spanning not more than approximately 400 kbp.

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“…Sixty five loci, which consist of 58 microsatellite-containing loci (marked with N in Table 1 including two loci for LSN) and seven additional loci (marked with asterisk in Table 2) were newly assigned on the rat chromosomes, while assignment of the remaining 81 genes was confirmed, except for PGl (no linkage) and PBPC2. (It was mapped to chromosome 1 by linkage analysis, although it has been previously reported to be located on chromosome 5; ZHANG et al 1988. ) It is also interesting to note that INSl has been shown by in situ hybridization, to map to the distal tip of chromosome 1, and provides thus a good telomeric anchor marker (MORI et al.…”

Section: Discussionmentioning

confidence: 99%

Rat gene mapping using PCR-analyzed microsatellites.

Serikawa¹,

Kuramoto²,

Hilbert³

et al. 1992

Genetics

332

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One hundred and seventy-four rat loci which contain short tandem repeat sequences were extracted from the GenBank or EMBL data bases and used to define primers for amplification by the polymerase chain reaction (PCR) of the microsatellite regions, creating PCR-formatted sequence-tagged microsatellite sites (STMSs). One hundred and thirty-four STMSs for 118 loci, including 6 randomly cloned STMSs, were characterized: (i) PCR-analyzed loci were assigned to specific chromosomes using a panel of rat x mouse somatic cell hybrid clones. (ii) Length variation of the STMSs among 8 inbred rat strains could be visualized at 85 of 107 loci examined (79.4%). (iii) A genetic map, integrating biochemical, coat color, mutant and restriction fragment length polymorphism loci, was constructed based on the segregation of 125 polymorphic markers in seven rat backcrosses and in two F2 crosses. Twenty four linkage groups were identified, all of which were assigned to a defined chromosome. As a reflection of the bias for coding sequences in the public data bases, the STMSs described herein are often associated with genes. Hence, the genetic map we report coincides with a gene map. The corresponding map locations of the homologous mouse and human genes are also listed for comparative mapping purposes.

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Mapping of rat prostatic binding protein genes C1, C2, and C3 to rat chromosome 5 by in situ hybridization

Cited by 11 publications

References 6 publications

The androgen-dependent rat prostatic binding protein: comparison of the sequences in the 5′ part and upstream region of the Cl and C2 genes and analysis of their transcripts

The androgen-dependent rat prostatic binding protein: comparison of the sequences in the 5′ part and upstream region of the Cl and C2 genes and analysis of their transcripts

All Human Genes of the Uteroglobin Family Are Localized on Chromosome 11q12.2 and Form a Dense Cluster

Rat gene mapping using PCR-analyzed microsatellites.

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