2003
DOI: 10.1007/s00705-002-0898-z
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Mapping of the bovine herpesvirus 1 glycoprotein C promoter region and its specific transactivation by the viral BICP27 gene product

Abstract: We investigated the cis-acting sequences of the promoter regulating the gene encoding the glycoprotein C (gC) of bovine herpesvirus 1 (BoHV-1), a gene of the gamma1 class. S1 nuclease protection assays revealed that gC transcription initiated predominantly at position C18281 of the viral genome, corresponding to 21 and 56 bases downstream from putative TATA-like and CAAT boxes, respectively. To map the gC promoter (pgC), we measured the ability of a series of nested deletions of the region +71 to -1155 with re… Show more

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Cited by 11 publications
(9 citation statements)
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“…All enzymes used for cloning procedures were purchased from Amersham Pharmacia Biotech (GE). The BICP27 ORF (Singh et al, 1996) and the minimal gC promoter sequence (Hamel and Simard, 2003) were amplified from BHV-1 genomic DNA by PCR using Deep Vent DNA polymerase (New England BioLabs). The primers for constructing all the recombinant plasmids are listed in Table 1.…”
Section: Plasmid Constructionmentioning
confidence: 99%
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“…All enzymes used for cloning procedures were purchased from Amersham Pharmacia Biotech (GE). The BICP27 ORF (Singh et al, 1996) and the minimal gC promoter sequence (Hamel and Simard, 2003) were amplified from BHV-1 genomic DNA by PCR using Deep Vent DNA polymerase (New England BioLabs). The primers for constructing all the recombinant plasmids are listed in Table 1.…”
Section: Plasmid Constructionmentioning
confidence: 99%
“…To address whether NLS, NoLS or both is critical for the transactivation activity of BICP27 on gC gene promoter, a PCR-based approach was used to isolate the BHV-1 gC promoter (Hamel and Simard, 2003). The promoter sequence was cloned into the pGL3 vector to generate pGL-gCp-Luc.…”
Section: Regulation Of Gc Promoter By Bicp27 and Its Nls Nols Or Botmentioning
confidence: 99%
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“…However, it is unclear how a putative interaction of KSHV Mta with the RNA polymerase II CTD could explain Mta's promoter specificity. Other Mta homologs within the Herpesviridae, including the human CMV UL69 protein, varicella-zoster virus 4 protein, bovine herpesvirus type 1 ICP27 protein, and equine herpesvirus type 1 ICP27 protein, all demonstrate transcriptional mechanisms of transactivation (36,69,77,88,106). The EICP27 protein physically interacts with and synergistically cooperates with the immediate-early EICP0 protein in transactivating viral promoters (2).…”
Section: Discussionmentioning
confidence: 99%
“…Furthermore, ICP27 is proven to be implicated in apoptosis, determining the composition of HSV-1 virions and inhibition of type I interferon signaling (Zachos et al, 2001;Melchjorsen et al, 2006;Johnson et al, 2008;Sedlackova and Rice, 2008;Gillis et al, 2009). To date, ICP27 and its other homologues have been extensively stud-ied, however, the exact functional properties of PRV UL54 are less understood, despite the fact that the aa sequence of UL54 shows 41% identity to ICP27 (Hamel and Simard, 2003;Boyne and Whitehouse, 2006;Melchjorsen et al, 2006;Boyne et al, 2008;Johnson et al, 2008;Sandri-Goldin, 2008;Toth and Stamminger, 2008;Boyne and Whitehouse, 2009;Guo et al, 2009;Juillard et al, 2009;Ding et al, 2010;Ote et al, 2010).…”
Section: Introductionmentioning
confidence: 99%