Marcadores moleculares RAPD e descritores morfológicos na avaliação da diversidade genética de goiabeiras (Psidium guajava L.)

Filho, Aroldo Gomes; Oliveira, Jurandi Gonçalves de; Viana, Alexandre Pío; Siqueira, Ana Paula de Oliveira; Oliveira, Marcos Góes; Pereira, Messias Gonzaga

doi:10.4025/actasciagron.v32i4.4720

Cited by 20 publications

(11 citation statements)

References 12 publications

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“…Random amplified polymorphic DNA (RAPD) markers are particularly useful when little is known about the genetic structure of a species. In this context, morphological data combined with molecular markers has been used to characterize the genetic diversity and variability (Gomes Filho et al, 2010). However, the quantification of phenotypic diversity among accessions is feasibly only when the phenotypic divergence reflects the genetic divergence (Dias and Kageyama, 1991) because environmental effects and gene action can complicate this assessment (Andrade et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of gabiroba based on random amplified polymorphic DNA markers and morphological characteristics

Assis¹,

Reis²,

Pinto³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. The fragmentation of the original vegetation of the Cerrado biome, caused by the expansion of agricultural areas, mainly in central-west Brazil, calls for an assessment of the native population of this vegetation, especially of the species of interest for domestication and sustainable use. The purpose of this study was to characterize the genetic diversity of 140 gabiroba mother plants (Campomanesia spp) and their progenies from 17 locations in Goiás. The morphological characteristics of the mother plants were evaluated, and the leaflets were collected for molecular analysis using 12 random amplified polymorphic DNA primers. The seed progenies of these matrices were transplanted to the field and morphologically evaluated. Distance matrices of the morphological data of the mother plants and progenies as well as the molecular data of the mother plants were constructed, and groups were formed using the Tocher method and the unweighted pair-group method based on arithmetic averages. The polymorphism level in the matrix was 90.44%. The greatest molecular distance (0.66) ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 12 (3): 3500-3509 (2013) Genetic diversity of gabiroba 3501was observed between mother plants from Santa Rita do Araguaia and Alexânia. By the Tocher method, 10, 13, and 17 groups were formed. The morphological evaluation of the mother plants and progenies as well as the molecular analysis of the mother plants showed genetic diversity. Significant genetic variability was detected in the progenies of the gabiroba base collection planted in Campus Jataí, Goiás.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity of gabiroba based on random amplified polymorphic DNA markers and morphological characteristics

Assis¹,

Reis²,

Pinto³

et al. 2013

Genet. Mol. Res.

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“…Junqueira et al, (2010) obteve resultados parecidos com a pitaya nativa do cerrado cujo polimorfismo foi de 95,06%. Em estudos com goiabeiras, Gomes Filho et al,(2010) obteve 87,97% e Pessanha et al, (2011) ao avaliar 3 regioes do Rio de Janeiro observou 97,5% de polimorfismo.…”

Section: Discussionunclassified

Estudo Da Variabilidade Genética De Uma População De Gabiroba (Campomanesia Adamantium), Utilizando Marcadores Rapd

Guaberto¹,

Veronezi²

2016

COLLOQ VITAE

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RESUMOEm estudos de conservação e seleção genética de espécies nativas da região do cerrado é comum a ocorrência natural da gabiroba (Campomanesia adamantium), pertencente à família Myrtaceae. As informações sobre a variabilidade genética dessas espécies são escassas. O objetivo foi estudar a variabilidade genética de duas populações de gabiroba provenientes das cidades de Martinópolis e Taciba no estado de São Paulo usando técnicas de RAPD. Os RAPDs apresentaram poder de discriminação eficiente entre os 20 genótipos analisados, com uma divergência genética variando de 38 a 78%. Significando que entre os indivíduos existe uma ampla base genética, possibilitando a manipulação desse material em programas de conservação. Dos 3 grupos formados, o Grupo I foi o que mais diferiu dos outros, apresentando maior variabilidade genética. O uso de marcadores RAPD apresentou ser eficaz no estudo de polimorfismo genético entre os indivíduos em questão. Palavras-chave: Myrtaceae, Fluxo Gênico, Marcadores Moleculares, Polimorfismo. STUDY OF THE GENETIC VARIABILITY OF TWO POPULATIONS OF GABIROBA (CAMPOMANESIA ADAMANTIUM), USING MOLECULAR MARKERS. ABSTRACTIn genetic conservation and selection of studies of native species of the Cerrado region it is common to naturally occurring gabiroba ( Campomanesia adamantium ) , belonging to the Myrtaceae family. Information on the genetic variability of the species are scarce. The objective was to study the genetic variability of two populations of gabiroba from the cities of Martinópolis and Taciba in São Paulo using RAPD techniques. The RAPDs presented efficient discriminating power among the 20 genotypes studied, with a genetic divergence ranging from 38 to 78%. Meaning that among individuals there is a broad base , allowing the handling of this material in conservation programs. Of the three formed groups , Group I was the one that differed from the others , with higher genetic variability. The use of molecular markers showed to be effective in the genetic polymorphism study of the individuals concerned

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“…In Psidium guajava, 117 bands were used to analyze the genetic diversity (Gomes-Filho et al, 2010). In Erythrina velutina, 106 bands were detected .…”

Section: Determining An Adequate Number Of Rapd Markers For Genetic Amentioning

confidence: 99%

Genetic analysis of Apuleia leiocarpa as revealed by random amplified polymorphic DNA markers: prospects for population genetic studies

et al. 2016

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ABSTRACT. Apuleia leiocarpa (Vogel) J.F. MacBride is a hardwood species native to South America, which is at serious risk of extinction. Therefore, it is of prime importance to examine the genetic diversity of this species, information required for developing conservation, sustainable management, and breeding strategies. Although scarcely used in recent years, random amplified polymorphic DNA markers are useful resources for the analysis of genetic diversity and structure of tree species. This study represents the first genetic analysis based on DNA markers in A. leiocarpa that aimed to investigate the levels of polymorphism and to select markers for the precise characterization of its genetic structure. We adapted the original DNA extraction protocol based on cetyltrimethyl ammonium bromide, and describe a simple procedure that can be used to obtain high-quality samples from leaf tissues of this tree. Eighteen primers were selected, revealing 92 bands, from which 75 were polymorphic and 61 were sufficient to represent the overall genetic structure of the population without compromising the precision of the analysis. Some fragments were conserved among individuals, which can be sequenced and used to analyze nucleotide diversity parameters through a wider set of A. leiocarpa individuals and populations. The individuals were separated into 11 distinct groups with variable levels of genetic diversity, which is important for selecting desirable genotypes and for the development of a conservation and sustainable management program. Our results are of prime importance for further investigations concerning the genetic characterization of this important, but vulnerable species.

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Marcadores moleculares RAPD e descritores morfológicos na avaliação da diversidade genética de goiabeiras (Psidium guajava L.)

Cited by 20 publications

References 12 publications

Genetic diversity of gabiroba based on random amplified polymorphic DNA markers and morphological characteristics

Genetic diversity of gabiroba based on random amplified polymorphic DNA markers and morphological characteristics

Estudo Da Variabilidade Genética De Uma População De Gabiroba (Campomanesia Adamantium), Utilizando Marcadores Rapd

Genetic analysis of Apuleia leiocarpa as revealed by random amplified polymorphic DNA markers: prospects for population genetic studies

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