ABSTRACT.We assessed the transferability of 120 EST-derived Eucalyptus microsatellite primers to Campomanesia adamantium and C. pubescens. Both species are berry trees native to the Brazilian Cerrado, and population genetic information is poor. Twelve markers were used to analyze the genetic variability of four sampled populations. Regarding DNA extraction, we sampled leaf tissues from two populations of each species (80 individuals). Of the 120 primers evaluated, 87 did not amplify any PCR products, and 21 rendered nonspecific amplification. Twelve primers were successfully transferred, providing a low combined probability of genetic identity for both species (5.718 x 10 -10 for C. adamantium; 1.182 x 10 -11 for C. pubescens) and a high probability of paternity exclusion (0.99939 for C. adamantium; 0.99982 for C. pubescens). The average number of alleles in the polymorphic loci was 6.8 for C. adamantium and 7.8 for C. pubescens, ranging from 2 to 16 alleles per locus. The observed heterozygosity values for C. adamantium and C. pubescens were 0.504 and 0.503, respectively, and the expected heterozygosity values for C. adamantium and C. pubescens were 0.517 and 0.579, respectively. The populations exhibited structured genetic variability with qP values of 0.105 for C. adamantium and 0.249 for C. pubescens. Thus, we concluded that these 12 microsatellite markers, transferred from Eucalyptus, were efficient for population genetic studies of C. adamantium and C. pubescens.

Rectal swabs of 198 Holstein × Gir crossbred beef cattle from 34 milk farms in the central west of Brazil were analyzed from August 2010 to February 2011. Strains of shiga toxin-producing Escherichia coli (STEC) were isolated from 72.73% (144/198) of the animals, on over 97% of the surveyed properties. The molecular characterization indicated the most common toxin gene stx1 in 70.88% of the animals (202/285), followed by 18.95% (54/285) stx1/sxt2, and 10.18% (29/285) stx2. The presence of STEC in animals together with the probable risk factors based on a questionnaire was evaluated in the owners of the evaluated animals. Results showed that the animal category "calves" and production/technification scale "low" of the farm were related to high STEC prevalence in cattle. The season did not significantly affect the presence of STEC in cattle. The STEC strains are considered a major pathogen, causing severe and potentially lethal diseases in humans such as hemorrhagic colitis and hemolytic uremic syndrome. This high prevalence of STEC in dairy cattle poses a significant risk to public health, since these microorganisms can contaminate products intended for human consumption, e.g., water, raw and pasteurized milk, meat products, dairy products, and/or products of plant origin.

resUmo: A palmeira Guariroba (Syagrus oleracea Becc.) é a única espécie produtora de palmito amargo o qual é muito apreciado na culinária Goiana, podendo também ser utilizada na alimentação animal e paisagismo, porém ainda é pouco estudada. A germinação lenta e desuniforme das sementes acarreta problemas na propagação dessa espécie, dificultando, principalmente, a produção de mudas em escala comercial. Nesta pesquisa, objetivou-se avaliar diferentes tratamentos destinados a reduzir o estado de dormência e, consequentemente, acelerar o período germinativo de sementes de guariroba no sentido de desenvolver técnicas de fácil metodologia para serem utilizadas na agricultura familiar. O delineamento experimental utilizado foi o de blocos inteiramente casualizados, com três repetições, em esquema fatorial 2x7, envolvendo dois fatores em estudo, semente (despolpada e não despolpada) e diferentes métodos de tratamento das sementes (7 métodos). Em uma segunda etapa, verificou-se a interferência da coleobroca na germinação das sementes, contabilizando as sementes atacadas em cada tratamento, avaliadas após a emergência. Os resultados observados mostram que os tratamentos a baixas temperaturas diminuem significativamente a germinação de sementes de guariroba e que o despolpamento das sementes promovem uma redução no tempo de germinação. Ainda foi constatado que uma redução da umidade até 8% não prejudica a germinação de sementes de guariroba e que a absorção de água pelas sementes é influenciada diretamente pelo tempo de permanência das mesmas em embebição, ocorrendo de forma independente da presença ou não de polpa. Nenhum dos tratamentos estudados mostrou efeito na redução do ataque da coleobroca.Palavras-chave: Guariroba, germinação, palmito amargo, embebição de sementes, desenvolvimento. effects of different treatments on dormancY of Syagrus oleraces Becc Palm seeds ABSTRACT: The Guariroba Palm (Syagrus oleraces Becc) is the only species known as producer of bitter palm heart, which is very appreciated in Goiânia cooking and can also be used in animal feeding and landscaping; however, it is little studied. The slow and uneven germination of seeds cause problems in the propagation of this species, thus complicating mainly the seedling production at a commercial scale. This study evaluated different treatments to reduce the dormancy state and consequently to speed up the germination period of guariroba palm seeds and looking for techniques of easy comprehension and application by small farmers. The experimental design was in completely randomized

-The objective of this work was to evaluate the genetic diversity of macaúba (Acrocomia aculeata) accessions from 35 municipalities of the state of Goiás, Brazil, in order to obtain information about the genetic variability and to provide subsidies for the commercial deployment of the culture in the Midwestern region of Brazil. The bunches were harvested with ripe fruits. The following were evaluated: fruit and almond lengths and diameters; the weights of fruits and almonds; the average number of almonds, as well as the oil contents in the almond and in the dehydrated fruit. The parameters that indicated significant contributions to the variability were fruit weight, fruit width, fruit length, and oil content in the almond.Index terms: Acrocomia aculeata, agroenergy, almond quality, biodiesel, divergence, oleaginous seed. Diversidade genética de frutos de macaúba provenientes de 35 municípios de GoiásResumo -O objetivo deste trabalho foi avaliar a diversidade genética de acessos de macaúba (Acrocomia aculeata) provenientes de 35 municípios de Goiás, a fim de obter informações sobre a variabilidade genética e fornecer subsídios para implantação comercial da cultura na região Centro-Oeste. Procedeu-se à colheita dos cachos com frutos maduros. Foram avaliados: os comprimentos e os diâmetros dos frutos e das amêndoas; os pesos dos frutos e das amêndoas; o número médio de amêndoas; bem como os teores de óleo na amêndoa e no fruto desidratado. Os parâmetros que indicaram contribuições significativas para a variabilidade foram peso do fruto, largura do fruto, comprimento do fruto e teor de óleo na amêndoa.Termos para indexação: Acrocomia aculeata, agroenergia, qualidade da amêndoa, biodiesel, divergência, semente oleaginosa.The search for viable alternatives for replacing fossil fuels by biodiesel is a growing concern, especially in relation to global climate change (Pimentel et al., 2016). The macaúba -Acrocomia aculeata (Jacq.) Lodd. ex Mart. -has been used as a raw material in the production of biofuels due to its high content of oil in the fruit, production of various energy residues, besides being a native perennial plant found naturally in almost all the Brazilian territory (Pimentel et al., 2015, Evaristo et al., 2016. The oil of the macaúba mesocarp is rich in oleic acid, and is demanded by both the food and the agroenergy industry (Coimbra & Jorge, 2011).Currently, a large part of macaúba cultivation is restricted to extractive activity, and the few plantations are not yet in full production, and others in some research centers (Oliveira et al., 2016). These research centers in Brazil have been evaluating the genetic potential of different genotypes through quantitative and qualitative analyses as well as physical and chemical analysis of macaúba fruits (Matsimbe et al., 2015).Thus, the macaúba is considered of great interest by the scientific community, especially for its oleaginous property, in relation to the possibility of obtaining genetically superior materials. This results in the identificatio...