Short Communication Validation of EST-derived microsatellite markers for two Cerrado-endemic Campomanesia (Myrtaceae) species

Miranda, Evelyn Alejandra; Novaes, Carolina Ribeiro Diniz Boaventura; Braga, Renato de Souza; Reis, Edésio Fialho dos; Pinto, Jefferson Fernando Naves; Telles, Mariana P. C.

doi:10.4238/gmr.15017658

Cited by 11 publications

(22 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Following the popularization and modernization of laboratory techniques for the analysis of genetic markers [6], primers developed for the detection of variability in accessions of plants of agricultural value have gradually been transferred to plants of ecological interest (e.g., [7][8][9][10][11]). With this, indices of genetic diversity (e.g., the number of alleles, number of haplotypes-k, haplotype diversity-h, nucleotide diversity-π, observed and expected heterozygosity-H O and H E ) and population structuring, such as F ST [12,13], R ST , G ST and θ ST , have been applied increasingly in other areas, such as phylogenetics, phylogeography, biogeography, molecular ecology, ecological genetics, genetic geography, and landscape genetics [14].…”

Section: Introductionmentioning

confidence: 99%

Recent Trends in Research on the Genetic Diversity of Plants: Implications for Conservation

et al. 2019

View full text Add to dashboard Cite

Genetic diversity and its distribution, both within and between populations, may be determined by micro-evolutionary processes, such as the demographic history of populations, natural selection, and gene flow. In plants, indices of genetic diversity (e.g., k, h and π) and structure (e.g., FST) are typically inferred from sequences of chloroplast markers. Given the recent advances and popularization of molecular techniques for research in population genetics, phylogenetics, phylogeography, and ecology, we adopted a scientometric approach to compile evidence on the recent trends in the use of cpDNA sequences as markers for the analysis of genetic diversity in botanical studies, over the years. We also used phylogenetic modeling to assess the relative contribution of relatedness or ecological and reproductive characters to the genetic diversity of plants. We postulated that genetic diversity could be defined not only by microevolutionary factors and life history traits, but also by relatedness, so that species more closely related phylogenetically would have similar genetic diversities. We found a clear tendency for an increase in the number of studies over time, confirming the hypothesis that the advances in the area of molecular genetics have supported the accumulation of data on the genetic diversity of plants. However, we found that the vast majority of these data have been produced by Chinese authors, and refer specifically to populations of Chinese plants. Most of the data on genetic diversity have been obtained for species in the International Union for Conservation of Nature (IUCN) category NE (Not Evaluated), which indicates a relative lack of attention on threatened species. In general, we observed very high FST values in the groups analyzed and, as we focused primarily on species that have not been evaluated by the IUCN, the number of plant species that are threatened with extinction may be much greater than that indicated by the listing of this organization. We also found that the number of haplotypes (k) was influenced by the type of geographic distribution of the plant, while haplotype diversity (h) was affected by the type of flower, and the fixation index (FST), by the type of habitat. The plant species most closely-related phylogenetically have similar levels of genetic diversity. Overall, then, it will important to consider phylogenetic dependence in future studies that evaluate the effects of life-history traits on plant genetic diversity.

show abstract

Section: Introductionmentioning

confidence: 99%

Recent Trends in Research on the Genetic Diversity of Plants: Implications for Conservation

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Recent research has validated the transferability of microsatellite markers from closely related species of C. adamantium , including expressed sequence tag (EST)–derived simple sequence repeat (SSR) markers from Eucalyptus L'Hér. (Myrtaceae) (Miranda et al., ; Crispim et al., ) and SSR primers from Eucalyptus spp., Eugenia uniflora L., and Melaleuca alternifolia Cheel (Fagundes et al., ). Analysis of genetic diversity using transferable molecular markers only reflects polymorphisms in conserved genomic regions among congeneric species or genera from the same family; however, transferable microsatellite markers from other species may be limited in the level of genetic diversity they can reveal in the target species (Queirós et al., ).…”

mentioning

confidence: 99%

Development and characterization of microsatellite markers in Campomanesia adamantium, a native plant of the Cerrado ecoregions of South America

et al. 2019

View full text Add to dashboard Cite

Premise A novel set of nuclear microsatellite markers was developed and characterized for Campomanesia adamantium (Myrtaceae) and tested for cross‐amplification in the related species C. sessiliflora. Methods and Results Forty‐one primer pairs were designed for simple sequence repeat loci, of which 36 successfully amplified and were polymorphic. The number of alleles ranged from two to 14, with an average of 8.14 alleles per locus. Additionally, cross‐amplification was tested in C. sessiliflora; more than 55.5% of the microsatellite loci amplified, confirming the use of these microsatellite markers in a related species. Conclusions We developed a set of microsatellite markers that will be useful for future studies of genetic diversity and population structure of C. adamantium and a closely related species, which will aid in future conservation efforts.

show abstract

“…Seven transferable EST-derived microsatellite markers developed by Grattapaglia et al [21] for Eucalyptus ssp. (EMBRA 1076, 1335, 1363, 1364, 1374, 1470, and 1811) were amplified by polymerase chain reaction (PCR) according to the conditions described by Miranda et al [18]. The length of the amplified fragments was determined using an automated sequencer (ABI3500).…”

Section: Microsatellite Markersmentioning

confidence: 99%

“…Research using molecular markers in guavira is still uncommon [17,18]. Therefore, population genetic studies are required to support conservation and breeding programs.…”

Section: Introductionmentioning

confidence: 99%

“…Ensuring genetic diversity can strongly influence the long-term viability of plant populations and their ability to adapt to changing climatic and environmental conditions. The degradation of the Cerrado biome, either by inadequate extractive exploitation of fruits or by the expansion of agricultural frontiers, may affect the genetic variability of Campomanesia [18]. Besides, plants that lack genetic diversity may be more susceptible to environmental stresses and less competitive with exotic invasive species.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Relationship between Genetic Variability and Land Use and Land Cover in Populations of Campomanesia adamantium (Myrtaceae)

et al. 2018

View full text Add to dashboard Cite

Campomanesia adamantium is an endemic plant of Cerrado biome that has potential for cultivation because its fruits have culinary and medicinal uses. However, genetic diversity studies using molecular markers with Cerrado species are scarce, and the inadequate extractive exploitation of fruits and the expansion of agricultural frontiers may also affect genetic variability. Therefore, studies in this field are of interest as they can provide sources for conservation and breeding programs. In this context, we investigated the genetic diversity of native populations of C. adamantium from different sites and the relationship between genetic variability and the land use and land cover of each site. A total of 207 plants were sampled in seven sites and characterized with seven polymorphic microsatellite markers. The use and coverage of land were mapped based on aerial images, and the land was classified into different categories. The genetic diversity was high in all populations, with low levels of differentiation due to allele sharing, mainly in Mato Grosso do Sul and Paraguay populations. The geographically closest populations were more genetically similar. The use and coverage of land indicated that intense agriculture promotes a significant decrease in genetic variability.

show abstract

Short Communication Validation of EST-derived microsatellite markers for two Cerrado-endemic Campomanesia (Myrtaceae) species

Cited by 11 publications

References 20 publications

Recent Trends in Research on the Genetic Diversity of Plants: Implications for Conservation

Recent Trends in Research on the Genetic Diversity of Plants: Implications for Conservation

Development and characterization of microsatellite markers in Campomanesia adamantium, a native plant of the Cerrado ecoregions of South America

Relationship between Genetic Variability and Land Use and Land Cover in Populations of Campomanesia adamantium (Myrtaceae)

Contact Info

Product

Resources

About