Marek's Disease Virus Infection in the Brain: Virus Replication, Cellular Infiltration, and Major Histocompatibility Complex Antigen Expression

Gimeno, Isabel M.; Witter, R. L.; Hunt, Henry D.; Lee, L. F.; Reddy, Sanjay M.; Neumann, Ulrich

doi:10.1354/vp.38-5-491

Cited by 64 publications

(47 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There- (26). Highly virulent strains of MDV-1, such as RB1B, cause a stronger MHC-I downregulation than less virulent strains or vaccine strains such as CVI988 (18). Nevertheless, downregulation of MHC-I was not detectable in chicken and human cells expressing the UL49.5 proteins of RB1B and CVI988.…”

Section: Discussionmentioning

confidence: 99%

“…MDV-1 is known to reduce MHC-I cell surface expression levels on infected cells (18,26). To assess whether MDV-1 UL49.5 is responsible for this phenomenon, we transiently expressed chicken hepatoma cells (LMH) and MJS cells with plasmids coding for the UL49.5 homologs of the MDV-1 strains RB1B and CVI988.…”

Section: Ul495 Of Mdv-1 Is Not Responsible For Mhc-i Downregulation mentioning

confidence: 99%

“…Serotype 1 of MDV is oncogenic, inducing T cell tumors in infected poultry. MHC-I downregulation has been observed on MDV-1-infected chicken cells (26,33) and in epithelial and infiltrating cells derived from brain tissue of infected chickens (18). To date, the responsible viral protein(s) have not been identified.…”

mentioning

confidence: 99%

See 2 more Smart Citations

The Capacity of UL49.5 Proteins To Inhibit TAP Is Widely Distributed among Members of the Genus Varicellovirus

Verweij

Lipińska

Koppers-Lalić

et al. 2011

J Virol

View full text Add to dashboard Cite

The lifelong infection by varicelloviruses is characterized by a fine balance between the host immune response and immune evasion strategies used by these viruses. Virus-derived peptides are presented to cytotoxic T lymphocytes by major histocompatibility complex (MHC) class I molecules. The transporter associated with antigen processing (TAP) transports the peptides from the cytosol into the endoplasmic reticulum, where the loading of MHC-I molecules occurs. The varicelloviruses bovine herpesvirus 1 (BoHV-1), pseudorabies virus, and equid herpesviruses 1 and 4 have been found to encode a UL49.5 protein that inhibits TAP-mediated peptide transport. To investigate to what extent UL49.5-mediated TAP inhibition is conserved within the family of Alphaherpesvirinae, the homologs of another five varicelloviruses, one mardivirus, and one iltovirus were studied. The UL49.5 proteins of BoHV-5, bubaline herpesvirus 1, cervid herpesvirus 1, and felid herpesvirus 1 were identified as potent TAP inhibitors. The varicella-zoster virus and simian varicellovirus UL49.5 proteins fail to block TAP; this is not due to the absence of viral cofactors that might assist in this process, since cells infected with these viruses did not show reduced TAP function either. The UL49.5 homologs of the mardivirus Marek's disease virus 1 and the iltovirus infectious laryngotracheitis virus did not block TAP, suggesting that the capacity to inhibit TAP via UL49.5 has been acquired by varicelloviruses only. A phylogenetic analysis of viruses that inhibit TAP through their UL49.5 proteins reveals an interesting hereditary pattern, pointing toward the presence of this capacity in defined clades within the genus Varicellovirus.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Ul495 Of Mdv-1 Is Not Responsible For Mhc-i Downregulation mentioning

confidence: 99%

See 1 more Smart Citation

The Capacity of UL49.5 Proteins To Inhibit TAP Is Widely Distributed among Members of the Genus Varicellovirus

Verweij

Lipińska

Koppers-Lalić

et al. 2011

J Virol

View full text Add to dashboard Cite

show abstract

“…These were absent in any of the birds infected with the very virulent RB1B strain. More recently, an acute paralysis has been described following parenteral infection with very virulent'/MDV, and this has been ascribed to lesions within the central nervous system (Gimeno et al ., , 2001Witter et al ., 1999). The immunopathology of the central nervous system in birds infected by different routes will be an important aspect for further study to enhance understanding of the pathobiology of MDV infection.…”

Section: Discussionmentioning

confidence: 99%

Route of challenge is critical in determining the clinical outcome of infection with a very virulent oncogenic herpesvirus, Marek's disease virus

et al. 2007

View full text Add to dashboard Cite

The majority of experimental studies examining Marek's disease virus infection have used parenteral injection of cell-associated virus. The aim of this study was to examine whether the route of entry of virus was critical in determining the outcome of infection. Susceptible (L7) and resistant (L6) White Leghorn chickens were infected with a very virulent Marek's disease virus, RB1B, by either the intra-abdominal or intra-tracheal route. Birds infected by the intra-tracheal route had earlier, higher or more sustained blood, spleen and lung viral concentrations than those infected by the intra-abdominal route. L7 birds had higher viral loads than L6 birds infected by the same route. Clinical outcomes reflected these data. Resistant birds infected by the intra-tracheal route had an increased prevalence of tumours and shorter survival times compared with those infected by the intra-abdominal route. Susceptible birds infected by the intra-tracheal route became paralysed 10 days after infection. L7 birds had shorter survival times and increased prevalences of tumours than L6 birds. The pathology and viraemia seen with intra-tracheal infection could not be fully replicated by increasing the dose in intra-abdominal infections. We conclude that instillation of infective dust produces a more aggressive infection that depends on the route of entry and form of virus, and not just on the challenge dose. Â© 2007 Houghton Trust Ltd

show abstract

“…An avidin Á/biotin Á/peroxidase complex (Vectastain † ABC kit; Vector Laboratories, Burlingame, California, USA) was used for immunohistochemistry as described elsewhere (Gimeno et al , 2001). In particular, the immunohistochemistry staining of the antigens Meq and CD30 were amplified with the tyramide signal reaction following the manufacturer's instruction for the TSA TM Biotin System Kit (PerkinElmer Life Science, Boston, Massachusetts, USA).…”

Section: Methodsmentioning

confidence: 99%

Novel criteria for the diagnosis of Marek's disease virus-induced lymphomas

Gimeno¹,

Witter²,

Fadly³

et al. 2005

Avian Pathology

Self Cite

View full text Add to dashboard Cite

Several novel criteria have been tested to assist in the differential diagnosis of tumours induced by Marek's disease virus (MDV) from those induced by avian leukosis virus (ALV) and reticuloendotheliosis virus (REV). A collection of tumours induced by inoculation of specific strains of MDV, ALV and REV, alone or in combination, were tested for quantification of MDV DNA by real-time polymerase chain reaction, expression of the MDV oncogene Meq, expression of several cell markers associated with transformation (CD30, Marek's disease-associated surface antigen, and p53), and level of DNA methylation in the tumour cells. In addition, tissues latently infected with MDV and non-infected tissues were tested as controls. Tumours induced by MDV had about 10 2 -fold more copies of MDV DNA than either tissues latently infected by MDV or tumours induced by retrovirus in MDV-vaccinated chickens. Moreover, the MDV antigen Meq was consistently expressed in all MDV tumours but it could not be detected in tissues latently infected with MDV or in tumours induced by retrovirus in MDV-vaccinated chickens. Other markers studied were not specific for MDV and therefore had limited value for diagnosis. Nonetheless, some of these markers might have potential value in research as they will help to identify transformed cells.

show abstract

Marek's Disease Virus Infection in the Brain: Virus Replication, Cellular Infiltration, and Major Histocompatibility Complex Antigen Expression

Cited by 64 publications

References 33 publications

The Capacity of UL49.5 Proteins To Inhibit TAP Is Widely Distributed among Members of the Genus Varicellovirus

The Capacity of UL49.5 Proteins To Inhibit TAP Is Widely Distributed among Members of the Genus Varicellovirus

Route of challenge is critical in determining the clinical outcome of infection with a very virulent oncogenic herpesvirus, Marek's disease virus

Novel criteria for the diagnosis of Marek's disease virus-induced lymphomas

Contact Info

Product

Resources

About