“…19,25 New imaging concepts, together with the availability of lasers and the use of computers have completely transformed optical microscopy. 24,26 Scanning confocal microscopy offers improved rejection of out-of-focus noise and provides greater resolution than conventional imaging, yielding greatly 20 Rhodamine B 1% saline solution, 3 h Confocal scanning microscope Sidhu et al 61 Rhodamine B 0.25% in distilled water, 3 h Two-photon laser technique Yoshikawa et al 63 Rhodamine B 1% saline solution, 3 h Confocal scanning microscope 64 Fluorescein 20% aqueous solution, 1 h, n/a n/a Kim et al 65 Rhodamine B 1% aqueous solution, n/a, n/a Conventional light microscope Harashima et al 66 Rhodamine 6 Rhodamine B isothiocyanate 1% in 50% alcoholic solution 20 8C/1 h Confocal scanning microscope Hossain et al 70 Rhodamine B n/a Conventional light microscope Kohara et al 55 Rhodamine B 0.6% during thermocycling n/a Palma-Dibb et al 4 Rhodamine B 0.2% aqueous solution, 24 h, n/a Optical microscope Pioch et al 54 Rhodamine B isothiocyanate 1% in 50% alcoholic solution, 24 h, 20 8C Confocal scanning microscope Corona et al 71 Rhodamine B 0.2%, aqueous solution, for 24 h, n/a Optical microscope de Almeida et al 52 Rhodamine 25 The intricate and often complicated methodology of specimen drying required for conventional SEM or TEM analysis, is not necessary for confocal microscopy. This advantage leads to a decreased risk of shrinkage or desiccation artifacts and allows the same specimens to be subsequently studied using other, additional microscopic techniques.…”