Margins of oral leukoplakia: autofluorescence and histopathology

Elvers, Dirk; Braunschweig, Till; Hilgers, Ralf-Dieter; Ghassemi, Alireza; Möhlhenrich, Stephan Christian; Hölzle, Frank; Gerressen, Marcus; Modabber, Ali

doi:10.1016/j.bjoms.2014.11.004

Cited by 15 publications

(10 citation statements)

References 25 publications

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“…Results were encouraging, demonstrating that the autofluorescence-based excision was successful in achieving a clear molecular margin when excising PMD ( Farah et al, 2018 ). These results confirmed those previously reported in literature, in which VELscope ® demonstrated that the actual sizes of some lesions are significantly larger than they look clinically ( Elvers et al, 2015 ).…”

Section: Velscope ®supporting

confidence: 92%

An Overview on Current Non-invasive Diagnostic Devices in Oral Oncology

et al. 2018

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Oral squamous cell carcinoma (OSCC) is the most common head and neck malignancy, and despite advances in cancer therapies, the overall 5-year survival rate has remained below 50% over the past decades. OSCC is typically preceded by potentially malignant disorders (PMD), but distinguishing high-risk from low-risk PMD is challenging. In the last years, several diagnostic methods as light-based detection systems (LBDS) have been proposed to facilitate the detection of OSCC and PMD. Furthermore, the recent evolution of nanotechnology may provide new opportunities to detect PMD and OSCC at an early stage. Indeed, several preclinical studies showed the potential of nanotechnology to enhance diagnostic accuracy. For these reasons, it is fundamental to conduct studies to evaluate the efficacy of nanotechnology implementation in LBDS. The aim of this article is to review the current literature on LBDS and to provide a summary of the sensitivity and specificity of each technique, and possible future applications of nanotechnologies. The LBDS showed great potential for screening and monitoring oral lesions, but there are several factors that hinder an extensive use of these devices. These devices seem to be useful in assessing lesion margins that must be biopsied. However, to date, conventional oral examination, and tissue biopsy remain the gold standard for OSCC diagnosis. The use of nanotechnologies could be the next step in the evolution of LBDS, thus providing devices that can help clinicians to detect and better monitor oral lesions.

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Section: Velscope ®supporting

confidence: 92%

An Overview on Current Non-invasive Diagnostic Devices in Oral Oncology

et al. 2018

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“…According to Jane-Salas et al [32], there are no clinical benefits throughout the use of VELscope ® on oral lesions diagnosis. Elvers et al [33] said that VELscope ® could diagnose lesions on mucosal inflammation loci, but this method could help clinicians to extend biopsy margins beyond visible margins. Hanken et al [34] said that VELscope ® could be a non-invasive and simple test to diagnose OPMD.…”

Section: Discussionmentioning

confidence: 99%

Early Diagnosis on Oral and Potentially Oral Malignant Lesions: A Systematic Review on the VELscope® Fluorescence Method

et al. 2019

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The fluorescence method is an innovative technique used by pathologists for examining body mucosa, and for the abnormalities tissue screening, potentially leading to the earlier discovery of pre-cancer, cancer or other disease processes. The early detection is one of the best mechanisms for enabling treatment success, increasing survival rates and maintaining a high quality of life. The purpose of this review is to evaluate the clinical efficiency of this diagnostic tool applied to the oral cavity (VELscope®). A literature systematic review has been performed. The initial research provided 53 results after applying the inclusion and exclusion criteria, and after a manual screening of the abstracts by the authors, only 25 results were eligible for review. The results and data contained in all the researches, no older than 10 years, were manually evaluated, and provided useful information on this diagnostic method. The VELscope® mean value about sensitivity and specificity resulted of 70.19% and 65.95%, respectively, by results analysis, but despite this some studies disagree about its clinical effectiveness, and this diagnostic method is still much debated in scientific and clinical medical literature. Surely being able to have efficient and effective tools from this point of view could help the clinician in the diagnosis, and also make timelier the pharmacological or surgical therapy, improving the quality of life of the patient, and in some cases guaranteeing a longer survival term.

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“…Over the past three decades, many researchers have reported on the use of autofluorescence visualization devices such as the VELscope for uses including not only screening for OSCC but other clinical applications such as the setting of margins during surgery for other types of cancer (Awan et al, ; Elvers et al, ; Lane et al, ; Onoyama et al, ; Poh et al, ; Poh et al, ; Scheer et al, ; Westra & Sidransky, ). In general, a reduction in FAD and NADH levels is considered to be a factor influencing the FVL image, with the reason being that cancer cells are often found to undergo a metabolic switch from favoring energy production through oxidative phosphorylation to energy production through aerobic glycolysis (the Warburg effect; Warburg, ).…”

Section: Discussionmentioning

confidence: 99%

“…A number of studies on the autofluorescence imaging method have been concerned with validating the differential diagnosis of OSCC and OPMD, by making comparisons between the FVL of the suspected lesion and cytodiagnosis or biopsy (Awan et al, ; Elvers et al, ; Ganga et al, ; Petruzzi et al, ; Scheer et al, ; Yamamoto et al, ). However, there is currently no standardized numerical cutoff value for assisting in the identification of tumors using such autofluorescence visualization devices.…”

Section: Introductionmentioning

confidence: 99%

The luminance ratio of autofluorescence in a xenograft mouse model is stable through tumor growth stages

Sumi

Umemura

Adachi

et al. 2018

Clinical & Exp Dental Res

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The aim of this research was to investigate the value of autofluorescence imaging of oral cancer across different stages of tumor growth, to assist in detecting tumors. A xenograft mouse model was created with human oral squamous cell carcinoma cell line HSC‐3 being subcutaneously inoculated into nude mice. Tumor imaging was performed with an autofluorescence imaging method (Illumiscan®) using the luminance ratio, which was defined as the luminance of the tumor site over the luminance of normal skin tissue normalized to a value of 1.0. This luminance ratio was continuously observed postinoculation. Tumor and normal skin tissues were harvested, and differences in the concentrations of flavin adenine dinucleotide and nicotinamide adenine dinucleotide were examined. The luminance ratio of the tumor sites was 0.85 ± 0.05, and there was no significant change in the ratio over time, even if the tumor proliferated and expanded. Furthermore, flavin adenine dinucleotide and nicotinamide adenine dinucleotide were significantly lower in tumor tissue than in normal skin tissue. A luminance ratio under 0.90 indicates a high possibility of tumor, irrespective of the tumor growth stage. However, this cutoff value was determined using a xenograft mouse model and therefore requires further validation before being used in clinical diagnosis.

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Margins of oral leukoplakia: autofluorescence and histopathology

Cited by 15 publications

References 25 publications

An Overview on Current Non-invasive Diagnostic Devices in Oral Oncology

An Overview on Current Non-invasive Diagnostic Devices in Oral Oncology

Early Diagnosis on Oral and Potentially Oral Malignant Lesions: A Systematic Review on the VELscope® Fluorescence Method

The luminance ratio of autofluorescence in a xenograft mouse model is stable through tumor growth stages

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