Abstract. The contribution of natural sources to ambient air particulate matter (PM) concentrations is often not considered; however, it may be significant for certain areas and during specific periods of the year. In the framework of the AIRUSE-LIFE+ project, state-of-the-art methods have been employed for assessing the contribution of major natural sources (African dust, sea salt and forest fires) to PM concentrations, in southern European urban areas. 24 h measurements of PM 10 and PM 2.5 mass and chemical composition were performed over the course of a year in five cities: Porto, Barcelona, Milan, Florence and Athens. Net African dust and sea-salt concentrations were calculated based on the methodologies proposed by the EC (SEC 2011/208). The contribution of uncontrolled forest fires was calculated through receptor modelling. Sensitivity analysis with respect to the calculation of African dust was also performed, in order to identify major parameters affecting the estimated net dust concentrations. African dust contribution to PM concentrations was more pronounced in the eastern Mediterranean, with the mean annual relative contribution to PM 10 decreasing from 21 % in Athens, to 5 % in Florence, and around 2 % in Milan, Barcelona and Porto. The respective contribution to PM 2.5 was calculated equal to 14 % in Athens and from 1.3 to 2.4 % in all other cities. High seasonal variability of contributions was observed, with dust transport events occurring at different periods in the western and eastern Mediterranean basin. Sea salt was mostly related to the coarse mode and also exhibited significant seasonal variability. Sea-salt concentrations were highest in Porto, with average relative contributions equal to 12.3 % for PM 10 . Contributions from uncontrolled forest fires were quantified only for Porto and were low on an annual basis (1.4 and 1.9 % to PM 10 and PM 2.5 , respectively); nevertheless, contributions were greatly increased during events, reaching 20 and 22 % of 24 h PM 10 and PM 2.5 concentrations, respectively.