Marine Fish Sperm Cryopreservation and Quality Evaluation in Sperm Structure and Function

Effect of extenders, cryoprotectants, and freezing methods on sperm quality and fertilisation rate of the European sea bass (Dicentrarchus labrax)

“…The cryoprotectants were maintained at a concentration of 10% (v/v), which has been reported to be effective for many species (Withler, 1982). Our results are consistent with previous reports showing that egg-yolk citrate and urea-egg-yolk with 10% DMSO produced the best post-thaw motility and viability, whereas glycerol performed poorly in this study and with other species such as mrigal (Cirrhinus cirrhosis), common carp (Cyprinus carpio L.) and red seabream (Pagrus major) (Rao, 1989;Hua et al, 2012).…”