Mariner and the ITm Superfamily of Transposons

Tellier, Michael; Bouuaert, Corentin Claeys; Chalmers, Ronald

doi:10.1128/microbiolspec.mdna3-0033-2014

Cited by 61 publications

(18 citation statements)

References 116 publications

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“…Mariner/Tc1/IS630 family transposases are unusual as they integrate their transposons, with a 2 bp stagger, strictly at TA target sequences (Tellier et al, 2015). They are widespread in nature and are used as tools for genome engineering and therapeutic applications.…”

Section: Introductionmentioning

confidence: 99%

A bend, flip and trap mechanism for transposon integration

Morris

Grey

McKenzie³

et al. 2016

eLife

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Cut-and-paste DNA transposons of the mariner/Tc1 family are useful tools for genome engineering and are inserted specifically at TA target sites. A crystal structure of the mariner transposase Mos1 (derived from Drosophila mauritiana), in complex with transposon ends covalently joined to target DNA, portrays the transposition machinery after DNA integration. It reveals severe distortion of target DNA and flipping of the target adenines into extra-helical positions. Fluorescence experiments confirm dynamic base flipping in solution. Transposase residues W159, R186, F187 and K190 stabilise the target DNA distortions and are required for efficient transposon integration and transposition in vitro. Transposase recognises the flipped target adenines via base-specific interactions with backbone atoms, offering a molecular basis for TA target sequence selection. Our results will provide a template for re-designing mariner/Tc1 transposases with modified target specificities.DOI: http://dx.doi.org/10.7554/eLife.15537.001

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Section: Introductionmentioning

confidence: 99%

A bend, flip and trap mechanism for transposon integration

Morris

Grey

McKenzie³

et al. 2016

eLife

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“…Usually, those elements represent a single open reading frame (ORF) coding for the transposase of ~350 amino acid (aa) residues. The ITm transposases possess a domain for site-specific DNA binding in N terminal part and a catalytic DDE/D domain in C terminal part (Tellier et al 2015). The sequences of ITm elements are typically flanked by a pair of terminal inverted repeats (TIRs) varying in length from 20 to 1900 bp.…”

Section: Introductionmentioning

confidence: 99%

“…Some ITm transposons additionally have the subterminal inverted repeats (SIRs) of variable length (175-1403 bp) (Claudianos et al 2002;Zhang et al 2016b). Despite the variability of TIRs, their terminy usually represent a TA dinucleotide target site duplication (TSD) (van Luenen and Plasterk 1994). ITm superfamily is divided into nine families: IS630, mariner, Tc1, pogo, maT, plants, mosquito, rosa and L31 (Shao and Tu 2001;Tellier et al 2015;Zhang et al 2016b;Puzakov et al 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Despite the variability of TIRs, their terminy usually represent a TA dinucleotide target site duplication (TSD) (van Luenen and Plasterk 1994). ITm superfamily is divided into nine families: IS630, mariner, Tc1, pogo, maT, plants, mosquito, rosa and L31 (Shao and Tu 2001;Tellier et al 2015;Zhang et al 2016b;Puzakov et al 2018). The classification of ITm elements is based on phylogenetic analysis and, until recently, it was well correlated with structural features (the length of peptide chain between the second and the third amino acid residues of the catalytic DDE/D triad (Tellier et al 2015).…”

Section: Introductionmentioning

confidence: 99%

“…ITm superfamily is divided into nine families: IS630, mariner, Tc1, pogo, maT, plants, mosquito, rosa and L31 (Shao and Tu 2001;Tellier et al 2015;Zhang et al 2016b;Puzakov et al 2018). The classification of ITm elements is based on phylogenetic analysis and, until recently, it was well correlated with structural features (the length of peptide chain between the second and the third amino acid residues of the catalytic DDE/D triad (Tellier et al 2015). However, the recent studies have identified, at least, four different groups of ITm elements with DD37E signature: mosquito (Shao and Tu 2001), TRT (Zhang et al 2016a), L18 and L31 (Puzakov et al 2018).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Why did theTc1-like elements of mollusks acquired the spliceosomal introns?

Пузаков

Puzakova

Чересиз

2019

Preprint

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Transposable elements are the DNA sequences capable of transpositions within the genome and, thus, exerting a considerable influence on the genome functioning and structure and providing the source of new genes. Transposable elements are classified into retrotransposons and the DNA transposons. IS630/Tc1/mariner superfamily of DNA transposons is one of the most diverse groups broadly represented among the eukaryotes. We identified a new group of Tc1-like elements in the mollusks, which we named TLEWI. These DNA transposons are characterized by the low copy number, the lack of terminal inverted repeats and the presence of DD36E signature and the spliceosomal introns in transposase sequence. Their prevalence among the mollusks is limited to subclass Pteriomorpha (Bivalvia). Since TLEWI possess the features of domesticated TE and the structure similar to the eukaryotic genes, which is not typical for the DNA transposons, we consider the hypothesis of co-optation of TLEWI gene by the bivalves.

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Prokaryotic DNA Transposons: Classes and Mechanism

Chandler

2017

Encyclopedia of Life Sciences

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Transposition plays a major role in genome plasticity causing chromosome rearrangements, deoxyribonucleic acid (DNA) insertion and deletion mutations and is key in gene sequestration and transmission by horizontal gene transfer. The processes play important roles in genome evolution and function. Transposable elements (TR) can be classified according to the chemistry of the transposition process itself: the nature of the catalytic mechanisms involved in the DNA cleavages necessary to liberate a copy of the transposon from its donor DNA and DNA strand transfer events (involved in insertion into a target DNA). This chapter is centred on prokaryotic TE, which use DNA intermediates for their movement. DNA transposable elements are extremely diverse, but, with a few exceptions, the mechanisms they use in their movement are common to both prokaryotic and eukaryotic TE. There are only a limited number of enzyme types that carry out DNA transposition. They are DDE (DED), Y, S, Y1 (HUH), Y2 (HUH) transposases, names of which are based on the active‐site residues involved in catalysis. More recently, an additional class, the casposons, has been identified in archaea and bacteria, which use a different type of sequence‐specific endonuclease. Transposition requires assembly of precise protein DNA complexes called transpososomes. Key Concepts Transposons are found in all living organisms. Transposons are diverse in organisation. Transposons use a limited number of enzyme types (transposases) for their movement. Transposases catalyse DNA cleavage in the donor DNA molecule and DNA strand transfer into the target DNA. Transposition requires an assembly of a precise protein–DNA complex, the transpososome. Transpososomes are assembled through a strictly ordered series of events in which the DNA–protein interactions position the DNA for catalysis.

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Mariner and the ITm Superfamily of Transposons

Cited by 61 publications

References 116 publications

A bend, flip and trap mechanism for transposon integration

A bend, flip and trap mechanism for transposon integration

Why did theTc1-like elements of mollusks acquired the spliceosomal introns?

Prokaryotic DNA Transposons: Classes and Mechanism

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