Marinilactibacillus psychrotolerans gen. nov., sp. nov., a halophilic and alkaliphilic marine lactic acid bacterium isolated from marine organisms in temperate and subtropical areas of Japan

Abstract

Marinilactibacillus psychrotolerans gen. nov., sp. nov., a halophilic and alkaliphilic marine lactic acid bacterium isolated from marine organisms in temperate and subtropical areas of Japan A novel marine lactic acid rod bacterium has been described for eight strains isolated from living and decomposing marine organisms collected from temperate and subtropical areas of Japan. The isolates were Gram-positive, catalase-negative, non-sporulating and motile with peritrichous flagella. They were slightly halophilic, highly halotolerant and alkaliphilic; the optimum NaCl concentration for growth was 2?0-3?75 % (w/v) with a range from 0 to 17?0-20?5 % (depending on the strain); the optimum pH was between 8?0 and 9?5 with a range from 6?0 to 10?0. They were psychrotolerant, growing well at 21?8˚C with a maximum at 40-45˚C and the optimum at 37-40˚C. Lactate yields were 87-100 % per consumed glucose; the residual products were formate, acetate and ethanol with a molar ratio of approximately 2 : 1 : 1. The product composition was markedly affected by the pH of fermentation medium; at higher pH, the yield of lactate decreased (60-65 % at pH 9?0) and that of other products increased conversely. The cell-wall peptidoglycan type was type A4b, Orn-D-Glu, whereas that of the genus Alkalibacterium, the phylogenetically closest lactic acid bacterium, was type A4b, Orn-D-Asp. The major cellular fatty acids were C16 : 0, C16 : 1D9, C18 : 0 and C18 : 1D9 (oleic acid). The G+C content of the DNA was 34?6-36?2 mol%. The eight isolates were phenotypically homogeneous and formed a single genomic species. The 16S rRNA gene sequence analysis indicated that the isolates constituted an independent phylogenetic lineage within the radiation of lactic acid bacteria with 96?2 % similarity to the genus Alkalibacterium. The secondary structure and the nucleotide sequence of the V6 region of the 16S rRNA were characteristic of the organism among other related lactic acid genera. On the bases of phenotypic and phylogenetic distinctness, the organism was proposed to belong to a new genus and species, Marinilactibacillus psychrotolerans gen. nov., sp. nov. The type strain, M13-2 T (G+C=36?2 mol%), has been deposited in the IAM, NBRC, NCIMB and NRIC culture collections as IAM 14980

Temporal Stability and Biodiversity of Two Complex Antilisterial Cheese-Ripening Microbial Consortia

“…By 16S rDNA analysis they were identified as Marinolactobacillus psychrotolerans. This species has never been isolated from redsmear cheese or any other dairy product, but only from marine organisms in Japan (22). Due to its ability to use lactose and its halophilic character, however, it obviously found a niche in the salty smear of a German milk product, representing an interesting case of Beijerinck's law (everything is everywhere, but the environment selects).…”

Caracterização taxonômica e fisiológica de bactérias ácido-láticas isoladas de pescado marinho

“…Algumas espécies de Lactobacillus também apresentam o crescimento limitado a baixas concentrações de NaCl, como, por exemplo, Lactobacillus sakei (4%) (MARCEAU et al, 2004), Lactobacillus delbrueckii (3,48%) e Lactobacillus bulgaricus (1,74%) (HUTKINS; EllEFSoN; KASHKET, 1987). Ishikawa et al (2003) Crescimento em diferentes concentrações salinas na presença de solutos orgânicos. o crescimento das três linhagens também foi analisado na presença de diferentes porcentagens de NaCl e de três solutos orgânicos testados individualmente.…”

Pisciglobus halotolerans gen. nov., sp. nov., isolated from fish sauce

“…In addition, they could be differentiated from the genera Carnobacterium, Marinilactibacillus, Isobaculum, Granulicatella, Atopobacter, Trichococcus and Vagococcus based on 16S rRNA gene sequence analyses as detailed above and as reported by Holley et al (2002), Ishikawa et al (2003), Lawson et al (2000), Liu et al (2002), and Collins et al (1987Collins et al ( , 2002. Therefore, strains C01 T and C02 are considered to represent a novel species of a new genus of the BacillusLactobacillus cluster, for which the name Pisciglobus halotolerans gen. nov., sp.…”