Marinobacter denitrificans sp. nov., isolated from marine sediment of southern Scott Coast, Antarctica

Zhang, Mingxia; Li, Anzhang; Wu, Qingping; Yao, Qing; Zhu, Honghui

doi:10.1099/ijsem.0.004120

Cited by 15 publications

(13 citation statements)

References 43 publications

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“…Data for strains 6–8 taken from Gauthier et al . [5], Zhang et al [7] and Kim et al . [27], respectively.…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

“…Data for strains 1-5 from this study. Data for strains 6-8 taken from Gauthier et al [5], Zhang et al [7] and Kim et al [27], respectively. +, Positive; −, negative; w, weak; nd, no data.…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

“…[5] with Marinobacter hydrocarbonoclasticus as the type species. At the time of writing, 57 species of the genus Marinobacter with validly published names have been isolated from different environments, such as sediments [6, 7], seawater [8–10], saline soil [11] and salt flat [12]. The aim of the present study was to determine the exact taxonomic position of strains PJ-16 T and PJ-38, which were isolated from a sea tidal flat.…”

Section: Introductionmentioning

confidence: 99%

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Marinobacter panjinensis sp. nov., a moderately halophilic bacterium isolated from sea tidal flat environment

Yang

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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Two moderately halotolerant bacterium strains, designated PJ-16T and PJ-38, were isolated from a tidal flat of the red beach in Panjin City, Liaoning Province, PR China. Cells were found to be Gram-stain-negative, aerobic, motile, rod-shaped with a single polar flagellum. Optimum growth of strain PJ-16T occurred at 30 °C, pH 7.0 and 0.2–8.0 % (w/v) NaCl, and strain PJ-38 at 30 °C, pH 6.0–7.0 and 0.2–8.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain PJ-16T was most closely related to Marinobacter denitrificans KCTC 62941T (99.2 % 16S rRNA gene sequence similarity), Marinobacter algicola DSM 16394T (98.6 %), Marinobacter salarius JCM 19399T (98.4 %) and Marinobacter confluentis KCTC 42705T (98.2 %), and strain PJ-38 was most closely related to M. denitrificans KCTC 62941T (99.1 %), M. algicola DSM 16394T (98.6 %), M. salarius JCM 19399T (98.4 %) and M. confluentis KCTC 42705T (98.1 %). The G+C content of the genomic DNA of strain PJ-16T based on its draft genomic sequence was 57.4 mol%. The major cellular fatty acids of strain PJ-16T were C16 : 0, C16 : 1 ω7c/C16 : 1 ω6c and C18 : 1 ω9c. The major respiratory quinone of PJ-16T was ubiquinone-9 and the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The results of the phenotypic, phylogenetic and genomic analyses revealed that strains PJ-16T and PJ-38 represent a novel species of the genus Marinobacter , and the name Marinobacter panjinensis sp. nov. is proposed. The type strain is PJ-16T (= CGMCC 1.13694T= KCTC 72023T).

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“…Data for strains 6–8 taken from Gauthier et al . [5], Zhang et al [7] and Kim et al . [27], respectively.…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Marinobacter panjinensis sp. nov., a moderately halophilic bacterium isolated from sea tidal flat environment

Yang

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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“…Most species of the genus Marinobacter are Gram-stain-negative, rod-shaped, motile by flagella, and halophilic. Marinobacter members have been isolated from various environments, such as marine sediments [4,5], saline soils [6], mangrove sediments [7], marine salterns [8], surface seawater [9], or offshore sediments [10]. Members of this genus require salt for their growth; therefore, they are slight or moderate halophiles [11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Marinobacter azerbaijanicus sp. nov., a moderately halophilic bacterium from Urmia Lake, Iran

Sánchez-Porro,

Aghdam,

Montazersaheb

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

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A novel moderately halophilic, Gram-stain-negative and facultatively anaerobic bacterium, designated as strain TBZ242T, was isolated from water of Urmia Lake in the Azerbaijan region of Iran. The cells were found to be rod-shaped and motile by a single polar flagellum, producing circular and yellowish colonies. The strain could grow in the presence of 0.5–10 % (w/v) NaCl (optimum, 2.5–5 %). The temperature and pH ranges for growth were 15–45 °C (optimum 30 °C) and pH 7.0–11.0 (optimum pH 8.0) on marine agar. The 16S rRNA gene sequence analysis revealed that strain TBZ242T belonged to the genus Marinobacter, showing the highest similarities to Marinobacter algicola DG893T (98.8 %), Marinobacter vulgaris F01T (98.8 %), Marinobacter salarius R9SW1T (98.5 %), Marinobacter panjinensis PJ-16T (98.4 %), Marinobacter orientalis W62T (98.0 %) and Marinobacter denitrificans JB2H27T (98.0 %). The 16S rRNA and core-genome phylogenetic trees showed that strain TBZ242T formed a distinct branch, closely related to a subclade accommodating M. vulgaris, M. orientalis, M. panjinensis, M. denitrificans, M. algicola, M. salarius and M. iranensis, within the genus Marinobacter. Average nucleotide identity and digital DNA–DNA hybridization values between strain TBZ242T and the type strains of the related species of Marinobacter were ≤85.0 and 28.6 %, respectively, confirming that strain TBZ242T represents a distinct species. The major cellular fatty acids of strain TBZ242T were C16 : 0 and C16 : 1 ω7c/C16 : 1 ω6c and the quinone was ubiquinone Q-9. The genomic DNA G+C content of strain TBZ242T is 57.2 mol%. Based on phenotypic, chemotaxonomic and genomic data, strain TBZ242T represents a novel species within the genus Marinobacter, for which the name Marinobacter azerbaijanicus sp. nov. is proposed. The type strain is TBZ242T (= CECT 30649T = IBRC-M 11466T). Genomic fragment recruitment analysis showed that this species prefers aquatic saline environments with intermediate salinities, being detected on metagenomic databases of Lake Meyghan (Iran) with 5 and 18 % salinity, respectively.

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“…Most members of the genus Marinobacter have been isolated from saline environments, such as sea water and sediment, tidal flats, saline soil, solar salterns and salt lakes [6][7][8][9][10][11][12] but they have also been isolated from sites as diverse as associated with diatoms [13], dinoflagellates [14,15] or sponges [16], from mangrove sediments [17], hydrothermal sediment [18], Antarctic environments [19][20][21][22] or from wine-barrel-decalcification wastewater [23]. Recently, 16S rRNA gene sequences related to this genus have also been reported in culture-independent studies in different habitats, such as an oil and gas reservoir [24], salt mines [25], associated with algae [26] or at Rio Tinto (Spain) [27].…”

Section: Introductionmentioning

confidence: 99%

Marinobacter iranensis sp. nov., a slightly halophilic bacterium from a hypersaline lake

Rafieyan,

Amoozegar,

Makzum

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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A novel halophilic bacterium, strain 71-iT, was isolated from Inche-Broun hypersaline lake in Golestan province, in the north of Iran. It was a Gram-stain-negative, non-endospore forming, rod-shaped bacterium. It grew at 4–40 °C (optimum 30 °C), pH 6.0–11.0 (optimum pH 7.5) and with 0.5–15 % (w/v) NaCl [optimum 3 % (w/v) NaCl]. The results of phylogenetic analyses based on the 16S rRNA gene sequence comparison indicated its affiliation to the genus Marinobacter and the low percentage of identity with the most closely related species (97.5 %), indicated its placement as a novel species within this genus. Digital DNA–DNA hybridization (dDDH) values and average nucleotide identity (ANI) analyses of this strain against closely related species confirmed its condition of novel taxon. On the other hand, the percentage of the average amino acid identity (AAI) affiliated strain 71-iT within the genus Marinobacter . The DNA G+C content of this isolate was 57.7 mol%. The major fatty acids were C16 : 0 and C16 : 1ω7c and/or C16 : 1 ω6c. Ubiquinone-9 was the major isoprenoid quinone and diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylethanolamine (PE) were the main polar lipids of this strain. On the basis of the phylogenomic and phenotypic (including chemotaxonomic) features, we propose strain 71-iT (= IBRC M 11023T = CECT 30160T = LMG 29252T) as the type strain of a novel species within the genus Marinobacter , with the name Marinobacter iranensis sp. nov. Genomic detections of this strain in various metagenomic databases indicate that it is a relatively abundant species in environments with low salinities (approximately 5 % salinity), but not in hypersaline habitats with high salt concentrations.

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Marinobacter denitrificans sp. nov., isolated from marine sediment of southern Scott Coast, Antarctica

Cited by 15 publications

References 43 publications

Marinobacter panjinensis sp. nov., a moderately halophilic bacterium isolated from sea tidal flat environment

Marinobacter panjinensis sp. nov., a moderately halophilic bacterium isolated from sea tidal flat environment

Marinobacter azerbaijanicus sp. nov., a moderately halophilic bacterium from Urmia Lake, Iran

Marinobacter iranensis sp. nov., a slightly halophilic bacterium from a hypersaline lake

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