Marker-assisted backcrossing using microsatellites and validation of SCAR Phs marker for resistance to white mold in common bean

Carneiro, Flávia Fernandes; Santos, João Bosco dos; Leite, Monik Evelin

doi:10.2225/vol13-issue6-fulltext-13

Cited by 5 publications

(2 citation statements)

References 17 publications

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“…Many studies have shown the potential of MAS in gaining resistance to many diseases in common beans, such (Carneiro et al, 2010 ;Ender et al, 2008), and bean common mosaic virus (Potyvirus; Miklas et al, 2000).…”

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confidence: 99%

Marker‐assisted backcrossing for disease resistance and agronomic traits in Carioca beans

et al. 2021

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Angular leaf spot, a disease caused by the fungus Pseudocercospora griseola, can lead to yield losses of up to 70% in the common bean (Phaseolus vulgaris) crop. Due to the quantitative character of angular leaf spot, a cultivar with high resistance depends on the presence of several resistance loci. In the present study, a markerassisted backcrossing approach was used involving an Andean donor parent (AND 277) and a Mesoamerican recurrent parent (IAC-Milênio). For marker-assisted backcrossing in the BC 1 F 1 genotypes, 15 molecular markers previously mapped for angular leaf spot resistance loci were used, and selection was performed by genotypes grouped with the donor parent in discrimination analysis of principal components. Through molecular selection, 42% of the BC 2 F 3 families selected for the Carioca grain ideotype showed high resistance to angular leaf spot under controlled conditions of infection. Two lines resistant under natural conditions and tolerant to Fusarium wilt (Fusarium oxysporum f. sp. phaseoli J. B. Kendr. & W. C. Snyder) were selected for the competition trial. The lines selected, AM138 and AM167, showed superiority to the recurrent cultivar for six of the eight agronomic traits, including resistance to Race 65 of Colletotrichum lindemuthianum slow grain darkening, early maturity, and lighter grain color. Both advanced lines will be included in the value for cultivation and use trials; and as Carioca lines with resistance of Andean origin, they can be widely used as sources of resistance in breeding programs to overcome problems related to inter-gene pool crosses.

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Section: Core Ideasmentioning

confidence: 99%

Marker‐assisted backcrossing for disease resistance and agronomic traits in Carioca beans

et al. 2021

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“…For example,Neeraja et al (2007) used 32 markers (<3 markers per chromosome) in background selection for developing submergence tolerance in rice Oliveira et al (2008). also used only 20 markers (<2 markers per chromosome) in MABC program in common bean (Phaseolus vulgaris L.), andCarneiro et al (2010) used only 25 markers distributed on nine of 11 chromosomes in background selection for improving resistance to white mold in common bean.…”

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confidence: 99%

Marker‐Assisted Backcrossing to Develop an Elite Cytoplasmic Male Sterility Line in Rice

et al. 2015

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Cytoplasmic male sterility (CMS) is a cornerstone of hybrid production in many crops. In three-line hybrid systems, use of CMS, maintainer, and fertility restorer lines is necessary for production of hybrid seeds. Limited resources of CMS and low variation of CMS lines cause genetic vulnerability to pathogens. Therefore, diversifying the CMS sources is indispensible for a sustainable production system of hybrid seed. In this study, we attempted for the first time to transfer CMS into maintainer line Yosen B in restricted generations using the marker-assisted backcrossing (MABC) method. The resultant F 1 hybrid of IR68897 A/Yosen B cross was backcrossed to Yosen B, and CMS plants in each backcross generation (from BC 1 F 1 to BC 3 F 1 ) were selected based on phenotyping test and MABC. Molecular assessment of backcross progenies was conducted using a mitochondrial CMS-specific marker and 34 polymorphic nuclear simple-sequence repeat (SSR) markers in early generations (from BC 1 F 1 to BC 2 F 1 ) and was continued using 9 additional SSRs and 82 inter-simple sequence repeat (ISSR) markers in BC 3 F 1 . A MABC strategy could successfully recover the recurrent parent genome (RPG) in BC 3 F 1 generation, and decreased heterozygosity of final CMS plants. Restorability test with known wild-abortive restorer lines (viz. IR36 and IR24) showed that combination of Yosen A  IR24 could produce highly fertile F 1 hybrid. Evaluation of some important agronomic traits of the final CMS line (BC 4 F 1 ) at field condition showed that it was comparable to the original maintainer fertile counterpart. Phenotypic and marker-assisted selections could considerably decrease the time needed for full recovery of RPG so that final CMS line could show a high similarity to original fertile counterpart.

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Phenotypic and genotypic screening of common bean (Phaseolus vulgaris L.) landraces for resistance to collar rot fungus (Sclerotium rolfsii Sacc.) in North of Iran

et al. 2019

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Marker-assisted backcrossing using microsatellites and validation of SCAR Phs marker for resistance to white mold in common bean

Cited by 5 publications

References 17 publications

Marker‐assisted backcrossing for disease resistance and agronomic traits in Carioca beans

Marker‐assisted backcrossing for disease resistance and agronomic traits in Carioca beans

Marker‐Assisted Backcrossing to Develop an Elite Cytoplasmic Male Sterility Line in Rice

Phenotypic and genotypic screening of common bean (Phaseolus vulgaris L.) landraces for resistance to collar rot fungus (Sclerotium rolfsii Sacc.) in North of Iran

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