Marker‐assisted breeding of Pi‐1 and Piz‐5 genes imparting resistance to rice blast in PRR78, restorer line of Pusa RH‐10 Basmati rice hybrid

Abstract

Rice blast, caused by fungus Magnaporthe grisea, is a serious disease causing considerable economic damage worldwide. Best way to overcome disease is to breed for disease-resistant cultivars/parental lines of hybrids. Pusa RH10, first aromatic, fine-grain rice hybrid released and cultivated extensively in India. Hybrid and its parental lines, Pusa 6A and PRR78, are highly susceptible to blast. CO39 pyramid carrying two dominant, broad-spectrum blast-resistance genes, viz. Pi-1 and Piz-5, used as a donor parent to introgress these genes into PRR78 using marker-assisted backcrossing (MABC). Microsatellite markers RM5926 and AP5659-5 tightly linked to Pi-1 and Piz-5 genes, respectively, were used for foreground selection to derive introgression lines. Further, these lines were evaluated for agronomic performance, disease reaction and cooking quality traits along with PRR78. Most of the improved lines were on par with PRR78 for all traits evaluated except gelatinization temperature. Recurrent parent genome percentage (RPG) study also revealed similarity of these lines with PRR78. Hybrids derived using improved PRR78 lines were superior over Pusa RH10 in terms of yield.

Rapid Introgression of the Fusarium Wilt Resistance Gene into an Elite Cabbage Line through the Combined Application of a Microspore Culture, Genome Background Analysis, and Disease Resistance-Specific Marker Assisted Foreground Selection

“…Naidoo et al (2012) observed that when transferring lpa1-1 to a maize inbred line, the restoration rate of the genetic background in BC 2 F 1 plants reached 84% using marker-assisted screening of the genomic background. Similar findings were reported for rice regarding resistance to Pyricularia oryzae (84% in BC 3 ; Gouda et al, 2013), tolerance to planthoppers, stem borers, leaf folders, and herbicides (95.8% in BC 3 F 4 ; Wan et al, 2014), and the breeding of pepper varieties rich in capsaicin ester (96% in BC 2 F 1 ; Jeong et al, 2015). In the current study, before the background analyses in background populations, genetic background of 01-20 and D134 were determined.…”

Micronutrient productivity: a comprehensive parameter for biofortification in rice (Oryza sativa L.) grain

“…Judicious integration of the conventional wisdom of plant breeding and the power of molecular markers would ensure continued success in complex breeding programs . Discerning the molecular genetics of a trait would also aid in the selection process, especially when dealing with traits that might manifest negative associations in germplasm accessions.…”

Introgression of Pi2 and Pi5 Genes for Blast (Magnaporthe oryzae) Resistance in Rice and Field Evaluation of Introgression Lines for Resistance and Yield Traits

“…Introgression lines showed resistance to leaf and neck blast at blast hot spots of Karnataka, which shelters maximum diversity of M. oryzae population (Gouda et al. ). Management of neck blast is equally important as that of leaf blast, as it causes direct grain loss by rotten necks and chaffy panicles.…”

“…Pi2 gene has been isolated and characterized, which encodes a NBS-LRR (a nucleotide-binding site and leucine-rich repeat) protein (Zhou et al 2006). Resistance conferred by Pi2 proved it to be an extreme useful gene in rice blast resistance programmes (Chen et al 1995;Liu et al 2002;Gouda et al 2012;). Pi5 gene on chromosome-9 encode proteins carrying three motifs characteristic of R genes: an Nterminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain and a leucine-rich repeat (LRR) motif associated with broad-spectrum resistance to diverse blast isolates (Jeon et al 2003;Wang et al 2013;Kishor et al 2015).…”