Marker-assisted introgression of a QTL region to improve rust resistance in three elite and popular varieties of peanut (Arachis hypogaea L.)

Abstract

Key messageSuccessful introgression of a major QTL for rust resistance, through marker-assisted backcrossing, in three popular Indian peanut cultivars generated several promising introgression lines with enhanced rust resistance and higher yield.AbstractLeaf rust, caused by Puccinia arachidis Speg, is one of the major devastating diseases in peanut (Arachis hypogaea L.). One QTL region on linkage group AhXV explaining upto 82.62 % phenotypic variation for rust resistance was validated and introgressed from cultivar ‘GPBD 4’ into three rust susceptible varieties (‘ICGV 91114’, ‘JL 24’ and ‘TAG 24’) through marker-assisted backcrossing (MABC). The MABC approach employed a total of four markers including one dominant (IPAHM103) and three co-dominant (GM2079, GM1536, GM2301) markers present in the QTL region. After 2–3 backcrosses and selfing, 200 introgression lines (ILs) were developed from all the three crosses. Field evaluation identified 81 ILs with improved rust resistance. Those ILs had significantly increased pod yields (56–96 %) in infested environments compared to the susceptible parents. Screening of selected 43 promising ILs with 13 markers present on linkage group AhXV showed introgression of the target QTL region from the resistant parent in 11 ILs. Multi-location field evaluation of these ILs should lead to the release of improved varieties. The linked markers may be used in improving rust resistance in peanut breeding programmes.Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-014-2338-3) contains supplementary material, which is available to authorized users.

Mapping QTLs for Leafspot Resistance in Peanut Using SNP-Based Next-Generation Sequencing Markers

“…Nonetheless, selected major QTLs identified in this study can be further confirmed for their use in molecular breeding to enhance resistance to leafspot disease, as it was performed previously for other peanut diseases, such as root-knot nematode and rust resistance (Burow et al 2014;Varshney et al 2014). Transferring one or more of these QTLs to different genetic backgrounds and developing near-isogenic lines (NILs) will help validate the usefulness of these loci for future breeding programs.…”

Genetic variability for total phenolics, flavonoids and antioxidant activity of testaless seeds of a peanut recombinant inbred line population and identification of their controlling QTLs

“…Marker assisted selection or introgression for major QTLs in peanut has been performed for rust resistance in peanut (Varshney et al 2014). Reports on this variation in recombinant inbred population and identification of genomic regions controlling such variation is valuable for marker assisted selection of peanut with enhanced amount of these bioactive compounds in seeds.…”

Advancements in molecular marker development and their applications in the management of biotic stresses in peanuts

“…However, over the last decade, significant developments have been made in the use of various molecular approaches for biotic stress management in peanuts, and new efforts such as functional genomics are likely to play key roles in the future Varshney et al, 2014;Gajjar et al, 2014). Recently, Kanyika et al (2015) has identified 376 polymorphic SSR markers in 16 African groundnut cultivars with a wide range of disease resistance.…”

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