2008
DOI: 10.2971/jeos.2008.09028
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Marker-free cell discrimination by holographic optical tweezers

Abstract: We introduce a method for marker-free cell discrimination based on optical tweezers. Cancerous, non-cancerous, and drug-treated cells could be distinguished by measuring the trapping forces using holographic optical tweezers. We present trapping force measurements on different cell lines: normal pre-B lymphocyte cells (BaF3; "normal cells"), their Bcr-Abl transformed counterparts (BaF3-p185; "cancer cells") as a model for chronic myeloid leukaemia (CML) and Imatinib treated BaF3-p185 cells. The results are com… Show more

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“…Firstly, the digital holographic microscopy is applied for accurately detection the axial position of the cells. The second step is cell classification based on combining escape-force measurement [123] with other cell identifications. By combining the two steps in one system, a reliable object detection and tracking system can be obtained.…”
Section: Other Methodsmentioning
confidence: 99%
“…Firstly, the digital holographic microscopy is applied for accurately detection the axial position of the cells. The second step is cell classification based on combining escape-force measurement [123] with other cell identifications. By combining the two steps in one system, a reliable object detection and tracking system can be obtained.…”
Section: Other Methodsmentioning
confidence: 99%