Marker profiles of human leukemia and lymphoma cell lines

Minowada, Jun; Koshiba, Hirofumi; Sagawa, Kimitaka; Kubonishi, Ichiro; Lok, M. S.; Tatsumi, E; Han, Tao; Srivastava, B. I. Sahai; Ohnuma, Takao

doi:10.1007/bf00405069

Cited by 100 publications

(68 citation statements)

References 24 publications

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“…Human non-T ALL cell lines Reh and Nalm 12 (11) were kindly provided by Dr. P. Ralph of the Memorial Sloan-Kettering Cancer Center. Cell line Josh-7 was derived from a patient with X-linked agammaglobulinemia (12).…”

Section: Methodsmentioning

confidence: 99%

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Induction of HLA-DC/DS (LEU 10) antigen expression by human precursor B cell lines.

Wang¹,

Al‐Katib²,

Lane³

et al. 1983

The Journal of Experimental Medicine

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The expression of HLA-DC/DS antigen detected by the monoclonal antibody Leu 10 was studied in three human precursor and pre-B cell lines (Josh 7, Reh, and Nalm 12). Flow cytometric analysis showed that none of these cell lines stained for the HLA-DC/DS antigen. In the presence of 1.6 X 10(-9) M of 12-O-tetradecanoylporbol-13-acetate (TPA), expression of this antigen was detected. The expression was completed after 168 h of incubation. Iodination of cell surface, immunoprecipitation by Leu 10 antibody, and two-dimensional gel analysis revealed that TPA-treated Josh 7 cells synthesized and expressed a 29,34 kD bimolecular complex with both alpha and beta chains different from those of HLA-DR antigen. Quantitative absorption experiments with cell lysates indicated a greater than 25-fold increase in HLA-DC/DS antigen in TPA-treated cells. With the induction of HLA-DC/DS antigen expression, there are concomitant decreases in the expression of the common acute lymphoblastic leukemia antigen (CALLA) and the enzymatic activity of terminal deoxynucleotidyl transferase. No appreciable changes in HLA-DR and Ig expression were observed. There was also no change in HLA-SB expression as detected by antibody ILR-1. However, DNA synthesis was markedly inhibited by TPA treatment. These results indicate that precursor and pre-B cell lines can be induced to mature in vitro. They also suggest that the expression of HLA-DC/DS antigen which precedes the expression of membrane Ig and follows the HLA-DR expression is relevant to human B cell development and cell interaction.

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Section: Methodsmentioning

confidence: 99%

“…By immunofluorescence, Josh 7 and Reh did not make any Ig whereas Nalm 12 made only cytoplasmic t~ chains. These cell lines represent human precursor and pre-B cell lines (11,12). Immunofluorescence studies as shown in Fig.…”

Section: Methodsmentioning

confidence: 99%

Induction of HLA-DC/DS (LEU 10) antigen expression by human precursor B cell lines.

Wang¹,

Al‐Katib²,

Lane³

et al. 1983

The Journal of Experimental Medicine

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“…The pre-B (CD19+ CD10 + Cm + /sIg À ) cell line NALM-6 and early B-cell/Burkitt's leukemia cell lines RAMOS, RAJI and DAUDI (CD19+sIgM+) (Uckun et al, 1991) were used in this study. The establishment, characterization and maintenance of these cell lines have been previously described in detail (Minowada et al, 1981). The leukemic T cell lines used in this study include, HuT 78 (Gootenberg et al, 1981), its clonal derivative H9 and Molt-3.…”

Section: Cell Lines and Patientsmentioning

confidence: 99%

Hypermethylation of the spleen tyrosine kinase promoter in T-lineage acute lymphoblastic leukemia

et al. 2003

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Sequence analysis of the noncoding first exon (exon 1) of the Syk gene demonstrated the presence of a previously cloned CpG island (GenBank #Z 65706). Transient transfection analysis in Daudi cells demonstrated promoter activity (18-fold increase over parental luciferase plasmid) for a 348 bp BstXI-BsrBI fragment containing this island. This region exhibits a high GC content (B75%), contains several SP1 binding sites and a potential initiator sequence, but lacks a strong TATA consensus. Bisulfite sequencing and methylation-specific PCR (MSP) of this region demonstrated that the Syk promoter CpG island was largely unmethylated in Blineage leukemia cell lines, control peripheral blood cells, human thymocytes and CD3 + T lymphocytes. However, dense methylation was seen in four T-lineage leukemia cell lines, Jurkat, H9, Molt 3 and HUT 78. MSP screening of leukemia cells from six T-lineage acute lymphoblastic leukemia (ALL) patients demonstrated methylation of the Syk promoter CpG island in one T-lineage ALL patient. Promoter methylation was correlated with reduced to absent expression of Syk mRNA and SYK protein in the T-lineage leukemia cell lines. Treatment of the leukemia lines Ha and Molt 3, with the methylation inhibitor, 5-aza-2 0 -deoxycytidine (5-aza-CdR) resulted in increased Syk mRNA expression. The presence of a methylated promoter sequence in these T-lineage leukemia cell lines and in one T-lineage patient suggests a potential role for SYK as a tumor suppressor in T-ALL.

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“…Indeed, certain lymphomas and certain leukemias such as chronic lymphatic leukemia are now considered by Berard et al (19) "simply to represent different clinical expressions of the same neoplastic process." Further, recent immunologic (20) …”

Section: Discussionmentioning

confidence: 99%

Critical assessment of epidemiologic studies on the human carcinogenicity of 1,3-butadiene.

Landrigan

1990

Environ Health Perspect

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,3-Butadiene, a major ingredient of synthetic rubber, has been shown to be carcinogenic in two animal species. To assess the possible human carcinogenicity of 1,3-butadiene, a critical review was undertaken of the epidemiologic literature.An early retrospective study of 8017 males employed in tire manufacturing found excess mortality for lymphatic and hematopoietic neoplasms in production workers (standardized mortality ratio, SMR = 560); these workers were exposed to 1,3-butadiene as well as to styrene and possibly to benzene. A recently updated epidemiologic study of 2568 workers at a butadiene manufacturing plant in Texas reported low mortality overall (SMR = 84) but found excess deaths for lymphosarcoma and reticulum cell sarcoma (SMR = 229). A retrospective study of workers employed at two synthetic rubber plants in Texas found excess mortality for lymphatic and hematopoietic malignancies in the older of these facilities; the excesses for lymphosarcoma (SMR = 224) and leukemia (SMR = 278) were most significant in wartime workers. A large, recently updated retrospective study of 12,113 workers employed in eight synthetic rubber manufacturing plants in the United States and Canada found excess mortality for lymphatic and hematopoietic cancer in production workers; the SMR for other lymphatic cancers in white production workers was 230, and the SMR for all lymphatic malignancies in black production workers was 507.These updated epidemiologic results strongly suggest an etiologic association between occupational exposure to 1,3-butadiene and human cancer. It is reasonable, therefore, to conclude that there now exists at least limited evidence for the human carcinogenicity of 1,3-butadiene.

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Marker profiles of human leukemia and lymphoma cell lines

Cited by 100 publications

References 24 publications

Induction of HLA-DC/DS (LEU 10) antigen expression by human precursor B cell lines.

Induction of HLA-DC/DS (LEU 10) antigen expression by human precursor B cell lines.

Hypermethylation of the spleen tyrosine kinase promoter in T-lineage acute lymphoblastic leukemia

Critical assessment of epidemiologic studies on the human carcinogenicity of 1,3-butadiene.

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