Markers of Endothelial Dysfunction: E-selectin, Endothelin-1 and von Willebrand Factor in Patients with Coronary Heart Disease, Including in Combination with Type 2 Diabetes Mellitus

Zhito, A V; Yusupova, A. O.; Privalova, E V; Khabarova, N V; Belenkov, Yu. N.

doi:10.20996/1819-6446-2019-15-6-892-899

Cited by 4 publications

(2 citation statements)

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“…It has been reported that the release of ET-1 is the main cause of various pathological stresses, for example, shear stress, hypoxia, angiotensin II, thrombin activation, and inflammatory cytokines . ET-1 is now known to be one of the markers of endothelial dysfunction . Diabetes mellitus is a common metabolic disease and it is a growing health problem worldwide.…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical Immunoassay of Endothelin-1 Based on a Fenton-Type Reaction Using Cu(II)-Containing Nanocomposites as Nanozymes

Tian

Zhao

et al. 2020

Anal. Chem.

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Endothelin-1 (ET-1) is a powerful endogenous vasoconstrictor and it is closely related to the pathogenesis of endothelial dysfunction that is commonly involved in the initiation of vascular inflammation and in the development of vascular diseases. A new method for the electrochemical immunoassay of ET-1 was put forward in this work. ET-1 antibodies (Ab), gold nanoparticles (GNPs), and copper ions were employed to synthesize nanoenzyme-labeled antibodies, Ab-GNPs-Cu(II) nanocomposites, and the latter was evaluated using transmission electron microscopy, dynamic light scattering, UV−vis absorption spectrophotometry, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy. These nanocomposites could be captured on a glassy carbon electrode (GCE) modified with poly(thionine) (PTH) and ET-1, GCE/PTH/ET-1. The immobilized nanoenzymes, GNPs-Cu(II) nanoparticles, played a peroxidase mimic role. Hydroxyl radicals, • OH, generated by a Fenton-type reaction, oxidized PTH and induced the considerable cathodic current on an assembled sandwich-type electrode. Owing to the competitive immunoreaction, ET-1 in the solution inhibited the capture of Ab-GNPs-Cu(II) nanocomposites. The deficiency of • OH caused the decline of the electrochemical response. The cathodic current change was in proportion to the ET-1 concentration from 0.5 to 500 ng mL −1 . Cell morphology and viability investigations show that human umbilical vein endothelial cells, HUVECs, suffered from dysfunction when they were incubated in the presence of high-concentration glucose. Analyses on the growth medium using the developed method reveal that ET-1 was secreted by the injured cells and the release level of ET-1 was associated positively with the glucose concentration in the growth medium.

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Section: Introductionmentioning

confidence: 99%

“…7 ET-1 is now known to be one of the markers of endothelial dysfunction. 8 Diabetes mellitus is a common metabolic disease and it is a growing health problem worldwide. In patients with diabetes, endothelial dysfunction appears to be a consistent finding.…”

Section: ■ Introductionmentioning

confidence: 99%

Electrochemical Immunoassay of Endothelin-1 Based on a Fenton-Type Reaction Using Cu(II)-Containing Nanocomposites as Nanozymes

Tian

Zhao

et al. 2020

Anal. Chem.

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Endothelial function in benign and progressive course of periferal arterial disease

Kalinin¹,

Suchkov²,

Chobanyan³

et al. 2021

Kardiol. serdechno-sosud. khir.

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Study of type 1 vascular adhesion molecules in patients with acute myocardial infarction with ST segment elevation at different body weights

Zakovryashina

Шлык

Garina

2021

jour

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Objective: to study the level of adhesion molecules in patients with STEMI with different BMI at the hospital stage and 12 months after the index event.Materials and methods: the study included 126 people with STEMI who had undergone PCI and 27 people in the control group. The analysis of the level of svcam‑1 in peripheral blood at the beginning of the disease and after 12 months was carried out, BMI and waist volume were measured. An assessment of the nature and frequency of complications after STEMI was performed.Results: the levels of biomarkers of the vascular endothelial adhesion molecule type 1 increase during the acute period of stemi, statistically signifi tly decrease, but remain increased by 3.5 times 12 months after the index event compared with the initial values. There was no association of VCAM‑1 with visceral obesity in the groups of our patients. Vascular endothelial adhesion molecules of type 1 increase in patients with a fatal outcome, as well as with an increase in the severity of OSN and CHF. Thus, VCAM 1 can be a predictor of an unfavorable outcome of AMI.Conclusions: the article presents the study of a marker of systemic inflammation VCAM‑11 in patients with STEMI with various types of obesity or BMI at the stage of hospitalization and outpatient follow‑up during the year. The determination of the VCAM‑1 level can be used to assess the intensity of the inflammatory process and the risk of adverse outcomes.

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Markers of Endothelial Dysfunction: E-selectin, Endothelin-1 and von Willebrand Factor in Patients with Coronary Heart Disease, Including in Combination with Type 2 Diabetes Mellitus

Cited by 4 publications

References 50 publications

Electrochemical Immunoassay of Endothelin-1 Based on a Fenton-Type Reaction Using Cu(II)-Containing Nanocomposites as Nanozymes

Electrochemical Immunoassay of Endothelin-1 Based on a Fenton-Type Reaction Using Cu(II)-Containing Nanocomposites as Nanozymes

Endothelial function in benign and progressive course of periferal arterial disease

Study of type 1 vascular adhesion molecules in patients with acute myocardial infarction with ST segment elevation at different body weights

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