Marking resection margins in surgical biopsy specimens.

Bull, A D; Start, R. D.; Smith, J. H. F.

doi:10.1136/jcp.44.3.262-a

Cited by 7 publications

(10 citation statements)

References 12 publications

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“…Surgical pathology specimens often contain clinically relevant tissue margins, and identification of these margins must be maintained from the initial gross examination through to the final microscopic evaluation. Various methods of marking these margins have been described, [1][2][3][4][5][6][7][8][9][10][11][12] and one of the most useful methods is the application of a tissue-marking dye (TMD) along the margin. Multiple colours may be used to denote different margins, especially in complex specimens with intersecting or adjacent margins.…”

Section: Introductionmentioning

confidence: 99%

Variable fidelity of tissue‐marking dyes in surgical pathology

Williams

Haché

2014

Histopathology

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Section: Introductionmentioning

confidence: 99%

Variable fidelity of tissue‐marking dyes in surgical pathology

Williams

Haché

2014

Histopathology

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“…The dotting method with varnish can replace the stitching technique or other techniques such as primitive drawings, or the use of alcian blue solution , colored gelatins ,, acrylic pigments , and Typp‐ex fluid for marking the resection margins ,. Indian ink is not very satisfactory due to the long drying time and the tendency of the ink to spread . Acetone or Bouin’s solutions have been used recently to aid in the drying of Indian ink –.…”

Section: Discussionmentioning

confidence: 99%

Application of derm dotting in oral and maxillofacial surgery

Hevele

Hauben

Haspeslagh

et al. 2015

Oral Science International

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a b s t r a c tPurpose: Derm dotting is a new method of marking skin biopsies, and it is used by dermatopathologists to identify most diagnostic tissues on a microscopic slide. This method uses nail varnish to mark specific lesions and suspected section planes, or to orient skin resections. The nail varnish is resistant to different tissue processes, discernible while cutting the tissue block, and easily recognizable under the microscope. We examined the effectiveness of derm dotting on intraoral tissues. Methods: We used the derm-dotting technique on the intraoral tissues of nine patients who underwent resection of a squamous carcinoma. We also tested this method on frozen sections. Results: In all cases of resected tissue, the nail varnish was visible under gross examination, traceable while cutting the tissue block, and clearly visible in the definitive sections. The dots were preserved in the frozen sections, but they were lost in half of the decalcified tissues. Conclusions: Derm dotting is an inexpensive, simple method that can replace the stitching technique used by surgeons to orient specimens. The stitches have to be removed by the pathologist, therefore possibly creating an artifact in the biopsy. The varnish dots or lines can be used to orient the specimen. In addition, the dots can easily mark suspect borders or areas of interest to be examined by the pathologist, using different colors, if desired. With derm dotting, the pathologist receives a more representative slide enabling a more accurate clinicopathological correlation.

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“…Several marking techniques are proposed in the literature [ 10 , 11 , 12 ]. The most commonly used colour is “India ink” [ 13 , 14 ]. As alternative methods, alcian blue solution or Whiteout (Tipp-Ex ® ), which was mentioned in 1990 for example by Harris [ 15 ], are described.…”

Section: Introductionmentioning

confidence: 99%

Alteration of Tissue Marking Dyes Depends on Used Chromogen during Immunohistochemistry

Kiefer

Huber

Füllgraf

et al. 2021

Cells

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Pathological biopsy protocols require tissue marking dye (TMD) for orientation. In some cases (e.g., close margin), additional immunohistochemical analyses can be necessary. Therefore, the correlation between the applied TMD during macroscopy and the examined TMD during microscopy is crucial for the correct orientation, the residual tumour status and the subsequent therapeutic regime. In this context, our group observed colour changes during routine immunohistochemistry. Tissue specimens were marked with various TMD and processed by two different methods. TMD (blue, red, black, yellow and green) obtained from three different providers (A, B and C, and Whiteout/Tipp-Ex®) were used. Immunohistochemistry was performed manually via stepwise omission of reagents to identify the colour changing mechanism. Blue colour from provider A changed during immunohistochemistry into black, when 3,3′-Diaminobenzidine-tetrahydrochloride-dihydrate (DAB) and H2O2 was applied as an immunoperoxidase-based terminal colour signal. No other applied reagents, nor tissue texture or processing showed any influence on the colour. The remaining colours from provider A and the other colours did not show any changes during immunohistochemistry. Our results demonstrate an interesting and important pitfall in routine immunohistochemistry-based diagnostics that pathologists should be aware of. Furthermore, the chemical rationale behind the observed misleading colour change is discussed.

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Marking resection margins in surgical biopsy specimens.

Cited by 7 publications

References 12 publications

Variable fidelity of tissue‐marking dyes in surgical pathology

Variable fidelity of tissue‐marking dyes in surgical pathology

Application of derm dotting in oral and maxillofacial surgery

Alteration of Tissue Marking Dyes Depends on Used Chromogen during Immunohistochemistry

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