Marrubium vulgare L. Leave Extract: Phytochemical Composition, Antioxidant and Wound Healing Properties

Abstract

Several factors contribute in wound generation, e.g., accidental traumas or surgery, and in certain cases, this dermal injury may have a devastating outcome. When skin damage occurs, the human body puts in place a sophisticated choreography, which involves numerous repairing processes to restore physiological conditions. Nevertheless, natural healing mechanisms are ineffective towards chronic or non-healing wounds and thus, therapeutic strategies may represent the only beneficial alternative to counteract these tissue insults. Over the years, numerous studies showed the great potential of plants in promoting wound healing, by virtue of their high contents in antioxidant species. These compounds trigger a molecular cascade that collimate into the promotion of reparative processes. In this article, we report on the potential effect on wound healing of Marrubium vulgare L., a medicinal plant well known for several pharmaceutical activities. To this aim, the methanolic extract was prepared and subjected to a phytochemical investigation, quantifying the amount of marrubiin via NMR and drawing the phytochemical fingerprint via high performance liquid chromatography—ultra violet/photodiode-array detection-electrospray/mass (HPLC-UV/PAD-ESI/MS) analysis. Lastly, the antioxidant properties and wound healing potential have been evaluated.

In vitro cytotoxicity evaluation of Marrubium vulgare L. methanol extract

“…Previous reported in vitro antioxidant activity studies, it was observed that M. vulgare aerial parts showed different and varying results. It can be concluded that this may due to the differences of the locations and extraction methods of the plant material [18][19][20][21]. In addition the plant material of the previous studies were either grown in cell culture or collected from various sites without standardization concerns, which is one of the major differences of our plant material, resulting difficult comparisons in this aspect.…”

Wound Healing: Contributions from Plant Secondary Metabolite Antioxidants

“…Throughout the world, a huge percentage of population depends upon the use of plantbased medicine because of their easy availability and also due to the lack of better healthcare alternatives. Plant-based medicines or herbal medicines have been effective and safe traditional methods practiced in many countries including China, India, and most African countries for the treatment of various diseases [2]. A large number of plant extracts, concoctions, poultices, decoctions, or pastes are equally used in many countries for treatment of diseases, cuts, wounds, and burns.…”

“…A large number of plant extracts, concoctions, poultices, decoctions, or pastes are equally used in many countries for treatment of diseases, cuts, wounds, and burns. Thus, since antiquity, several medicinal plants and plant-based strategies are widely known for their significant role in wound healing and skin regeneration as well as their therapeutic applications [2].…”

The use of a microwave‐assisted solvent extraction coupled with HPLC‐UV/PAD to assess the quality of Marrubium vulgareL. (white horehound) herbal raw material

“…Recent literature has evidenced that Marrubium vulgare shows several in vivo and in vitro activities including antihypertensive, antioxidant, antiinflammatory, antidiabetic, effects on respiratory system, digestive stimulant, antiasthmatic, hypolipidemic, antibacterial, and antifungal effects. Within this context, and as a part of our current research in this field, in the present contribution we focused on M. vulgare L. (white/common horehound), an annual herbaceous plant belonging to Lamiaceae family, native to Eurasia and northern Africa zones . Due to its biological activities, the European Medicines Agency has published a monograph for M. vulgare in which all the traditional, non‐clinical and clinical data are reported .…”