Marshall Barber and the century of microinjection: from cloning of bacteria to cloning of everything

Korzh, Vladimir; Strähle, Uwe

doi:10.1046/j.1432-0436.2002.700601.x

Cited by 25 publications

(22 citation statements)

References 35 publications

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“…First widely employed in bacteriology to isolate single cells, microsurgical procedures relied on micromanipulatorsinstruments that converted the manipulation of screws, knobs or joy-stick-style controls into microscopic three-dimensional movements of capillary needles or pipettes. 58 The first commercially available micromanipulators appeared in the 1930s; by the 1960s Leitz models dominated the market. 59 If much early microscopic work in experimental embryology had been done by directly holding instruments, nuclear transfer and chimera experiments relied on motion aided by amicromanipulator.…”

Section: Tinkering With Microinjectionmentioning

confidence: 99%

Tinkering with genes and embryos: the multiple invention of transgenic mice c. 1980

Myelnikov

2019

History and Technology

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Genetically modified or 'transgenic' mice are a routine experimental tool in biomedical research, commonly produced by injecting DNA into one-cell embryos. These animals were independently invented in 1980 by multiple university groups in the United States and Europe that combined expertise in mouse developmental biology and recombinant DNA techniques, or 'genetic engineering'. In this article, I examine this multiple invention and argue that research strategies, experimental practices, and funding arrangements that led to transgenic mice are best described as tinkering. These creative and speculative endeavors, combined with partial knowledge of what was happening in competing laboratories, created a fruitful atmosphere for research which led to the multiple invention. The tinkering was, however, underpinned by infrastructures that were crucial to success, some long established, such as mouse supply or embryological tools, and some emerging, such as the informal exchange of isolated genes.

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Section: Tinkering With Microinjectionmentioning

confidence: 99%

Tinkering with genes and embryos: the multiple invention of transgenic mice c. 1980

Myelnikov

2019

History and Technology

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“…Direct penetration employs a solid conduit or vehicle to concurrently penetrate the membrane and introduce cargo. Prevalent samples here are mechanical cell membrane penetration (micro- and nano-injection) [ 33 ] and gene gun with ballistic particles [ 34 ] as shown in Figure 2 . Unlike carrier-based delivery, active external force is utilized for puncturing the cell membrane to obtain access in direct penetration, where the target cell must respond to repair damage sustained to the plasma membrane or other cellular structures.…”

Section: Introductionmentioning

confidence: 99%

A Review on Electroporation-Based Intracellular Delivery

et al. 2018

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Intracellular delivery is a critical step in biological discoveries and has been widely utilized in biomedical research. A variety of molecular tools have been developed for cell-based gene therapies, including FDA approved CAR-T immunotherapy, iPSC, cell reprogramming and gene editing. Despite the inspiring results of these applications, intracellular delivery of foreign molecules including nucleic acids and proteins remains challenging. Efficient yet non-invasive delivery of biomolecules in a high-throughput manner has thus long fascinates the scientific community. As one of the most popular non-viral technologies for cell transfection, electroporation has gone through enormous development with the assist of nanotechnology and microfabrication. Emergence of miniatured electroporation system brought up many merits over the weakness of traditional electroporation system, including precise dose control and high cell viability. These new generation of electroporation systems are of considerable importance to expand the biological applications of intracellular delivery, bypassing the potential safety issue of viral vectors. In this review, we will go over the recent progresses in the electroporation-based intracellular delivery and several potential applications of cutting-edge research on the miniatured electroporation, including gene therapy, cellular reprogramming and intracellular probe.

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“…Barber’s methods were soon noticed by German Nobel Laureate Heinrich Hermann Robert Koch, who subsequently visited the United States in 1908 and observed a demonstration by Barber at the Sixth International Congress on Tuberculosis in Washington, DC (KU History, 2016). Albert Prescott Mathews, Professor of Physiological Chemistry in the Department of Physiology at the University of Chicago, was also aware of Barber’s work and sent Research Fellow George Lester Kite to Kansas to learn the micropipette technique in about 1912 (Terreros and Grantham, 1982; Korzh and Strähle, 2002). As we shall see, these are not the last times that the University of Kansas and the Physiology Department at the University of Chicago feature in the micropipette story.…”

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confidence: 99%

“…Publications from around this time, and later, indicate that Barber, Chambers, and Charles Vincent Taylor (Fig. 9) in the United States and Péterfi in Europe held each other’s technical and scientific expertise in high esteem while examples of equipment (including that via Janse and another from Barber to Robert Koch, according to Korzh and Strähle [2002]) passed each way between them. In his obituary of Péterfi, Robert Chambers (Chambers and Maskar, 1953) noted that the perfected Péterfi-Micromanipulator, manufactured by Carl Zeiss, had become the most widespread instrument of its kind in the world.…”

mentioning

confidence: 99%