ZnO-NPs loaded polyvinylidene
fluoride (PVDF) composite nanofibers
were fabricated by electrospinning and optimized using different concentrations
(0, 2, and 5 wt %) of ZnO-NPs. Characterization techniques, for example,
FTIR, SEM, XRD, and tensile strength analysis were performed to analyze
the composite nanofibers. Molecular docking calculations were performed
to evaluate the binding affinity of PVDF and ZnO@PVDF against the
hexon protein of adenovirus (PDB ID: 6CGV). The cytotoxicity of tested materials
was evaluated using MTT assay, and nontoxic doses subjected to antiviral
evaluation against human adenovirus type-5 as a human respiratory
model were analyzed using quantitative polymerase chain reaction assay.
IC
50
values were obtained at concentrations of 0, 2, and
5% of ZnO-loaded PVDF; however, no cytotoxic effect was detected for
the nanofibers. In 5% ZnO-loaded PVDF nanofibers, both the viral entry
and its replication were inhibited in both the adsorption and virucidal
antiviral mechanisms, making it a potent antiviral filter/mask. Therefore,
ZnO-loaded PVDF nanofiber is a potentially prototyped filter embedded
in a commercial face mask for use as an antiviral mask with a pronounced
potential to reduce the spreading of infectious respiratory diseases,
for example, COVID-19 and its analogues.