Maspin expression in early oral tongue cancer and its relation to expression of mutant-type p53 and vascular endothelial growth factor (VEGF)

Cho, Jae Hoon; Kim, Hyung Seok; Park, Chang Soo; Kim, Jin Kook; Jung, Kwang Yoon; Shin, Bong Kyoung; Kim, Han Kyum

doi:10.1016/j.oraloncology.2006.03.018

Cited by 27 publications

(25 citation statements)

References 18 publications

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“…It is also possible that the role of maspin differs according to the cell type. However, the majority of studies of OSCC (22,24), including the present study, indicate that maspin has a protective role against the oncogenic process, although certain investigators found no correlation (23) or even reached the opposite conclusion (9).…”

Section: Discussioncontrasting

confidence: 65%

“…Studies on maspin have almost exclusively investigated established malignant lesions and have published contradictory results. Certain studies revealed no correlation between protein expression and prognosis (23), while other studies correlated its overexpression with a better (22,24) or worse (9) prognosis of carcinoma lesions. These differences may be in part due to the fact that maspin is primarily expressed in the cytoplasm, but may also be observed in the nucleus, secretory vesicles and cell surface, thus this subcellular partition may affect the function of this protein (22).…”

Section: Discussionmentioning

confidence: 98%

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Differences in the expression of five senescence markers in oral cancer, oral leukoplakia and control samples in humans

Bascones-Martínez¹,

López-Durán²,

Cano‐Sánchez³

et al. 2012

Oncology Letters

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Abstract. Oncogene-induced senescence (OIS) may be a response to oncogenic activation, acting as a natural barrier against carcinogenesis at a premalignant stage. Thus, numerous cells in premalignant lesions enter senescence, but none or few in malignant tumours. This event could be due to the loss of senescence pathway effectors, including p16 (INK4a)-pRb or ARF-p53. The aim of this study was to characterize and compare the expression of certain senescent markers between oral precancer and cancer tissue samples. The expression of cyclin D1, Rb, maspin, p53 and mouse double minute 2 (MDM2) was analyzed in 20 paraffin-embedded tissue samples of normal oral mucosa (NOM), 14 samples of oral leukoplakia without dysplasia (OLD-), 11 samples of leukoplakia with dysplasia (OLD+) and 15 samples of oral squamous cell carcinoma (OSCC) by immunohistochemistry in tissue arrays. The expression of p16-pRb pathway markers, cyclin D1, maspin and Rb, was more frequent in OLD+ samples than in OSCC samples, although a statistical significance was only observed for maspin (P=0.036). Cyclin D1 expression was also significantly more frequent in OLD-samples vs. NOM samples. For the ARF-p53 pathway, the expression of p53 and MDM2 was significantly more frequent in the OLD-samples compared to in the NOM ones. These findings may indicate a role for cellular senescence in oral carcinogenesis, considering maspin as a reliable senescence marker and prognostic factor in oral premalignant lesions.

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Section: Discussioncontrasting

confidence: 65%

Section: Discussionmentioning

confidence: 98%

Differences in the expression of five senescence markers in oral cancer, oral leukoplakia and control samples in humans

Bascones-Martínez¹,

López-Durán²,

Cano‐Sánchez³

et al. 2012

Oncology Letters

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“…VEGF-A has been shown in breast cancer to constitute a marker of angiogenesis, correlating with vessel density, predict a worse prognosis, and constitute an effective target of molecular therapy [31]. Correlation of Maspin and VEGF-A expression have been reported in other tumor types [36][37][38], but to the best of our knowledge this is the first study to find this link in breast cancer. We observed a significant association between loss of Maspin expression and cytoplasmic accumulation of VEGF-A, suggesting the role of Maspin in angiogenesis.…”

Section: Discussionmentioning

confidence: 84%

Clinical significance of Maspin promoter methylation and loss of its protein expression in invasive ductal breast carcinoma: correlation with VEGF-A and MTA1 expression

et al. 2010

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Maspin is a serine protease inhibitor with tumor-suppressor activity. Maspin can suppress tumor growth and metastasis in vivo and tumor cell motility and invasion in vitro. Previous studies indicate that the loss of Maspin expression is closely linked to aberrant methylation of the Maspin promoter. We examined the promoter methylation status of Maspin in tumor and corresponding serum of breast cancer patients. In addition, protein expression of this gene was also assessed to determine possible correlation between promoter hypermethylation and gene silencing. Further, we investigated the correlation of Maspin expression with vascular endothelial growth factor (VEGF-A) and MTA1 expression. Maspin methylation was analyzed by methylation-specific PCR in 100 invasive ductal breast carcinoma patients' tumors and circulating DNA in a prospective study. Promoter hypermethylation was correlated with expression of the encoded protein in tumors by immunohistochemistry. Significant correlation was observed between promoter hypermethylation of Maspin (r = +0.88; p ≤ 0.0001) in tumors and paired sera. Significant association was found between Maspin promoter hypermethylation and loss of its protein expression (p = 0.01, OR = 3.1, 95% CI = 1.3-7.4). The expression of VEGF-A and MTA1 was lower in tumors with high Maspin expression compared to tumors with loss of Maspin expression. Our results indicate that aberrant promoter methylation is associated with loss of Maspin immunoreactivity in breast cancer tissues. Further, loss of Maspin expression is significantly correlated with increased expression of VEGF-A and MTA1.

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“…In a similar manner, the reduction we found for Maspin is expected. This as Maspin is a tumour supressor protein that was shown to suppress tumour growth and progression, angiogenesis, invasion and metastasis in various malignancies including head and neck cancer (Cho et al, 2007;Iezzi et al, 2007;Marioni et al, 2008). Accordingly, its reduction is expected to promote carcinogenesis.…”

Section: Discussionmentioning

confidence: 99%

Salivary analysis of oral cancer biomarkers

et al. 2009

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BACKGROUND: Oral cancer is a common and lethal malignancy. Direct contact between saliva and the oral cancer lesion makes measurement of tumour markers in saliva an attractive alternative to serum testing. METHODS: We tested 19 tongue cancer patients, measuring the levels of 8 salivary markers related to oxidative stress, DNA repair, carcinogenesis, metastasis and cellular proliferation and death. RESULTS: Five markers increased in cancer patients by 39 -246%: carbonyls, lactate dehydrogenase, metalloproteinase-9 (MMP-9), Ki67 and Cyclin D1 (CycD1) (Pp0.01). Three markers decreased by 16 -29%: 8-oxoguanine DNA glycosylase, phosphorylated-Src and mammary serine protease inhibitor (Maspin) (Pp0.01). Increase in salivary carbonyls was profound (by 246%, P ¼ 0.012); alterations in CycD1 (87% increase, P ¼ 0.000006) and Maspin (29% decrease, P ¼ 0.007) were especially significant. Sensitivity values of these eight analysed markers ranged from 58% to 100%; specificity values ranged from 42% to 100%. Both values were especially high for the CycD1 and Maspin markers, 100% for each value of each marker. These were also high for carbonyls, 90% and 80%, respectively, and for MMP-9, 100% and 79%, respectively. CONCLUSIONS: The significance of each salivary alteration is discussed. As all alterations correlated with each other, they may belong to a single carcinogenetic network. Cancer-related changes in salivary tumour markers may be used as a diagnostic tool for diagnosis, prognosis and post-operative monitoring.

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Maspin expression in early oral tongue cancer and its relation to expression of mutant-type p53 and vascular endothelial growth factor (VEGF)

Cited by 27 publications

References 18 publications

Differences in the expression of five senescence markers in oral cancer, oral leukoplakia and control samples in humans

Differences in the expression of five senescence markers in oral cancer, oral leukoplakia and control samples in humans

Clinical significance of Maspin promoter methylation and loss of its protein expression in invasive ductal breast carcinoma: correlation with VEGF-A and MTA1 expression

Salivary analysis of oral cancer biomarkers

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