Mass spectrometric analysis for aromatic compounds in bile of fish sampled after the Exxon Valdez oil spill

Krahn, Margaret M.; Burrows, Douglas G.; Ylitalo, Gina M.; Brown, Donald W.; Wigren, Catherine A.; Collier, Tracy K.; Chan, Sin Lam; Varanasi, Usha

doi:10.1021/es00025a012

Cited by 114 publications

(43 citation statements)

References 13 publications

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“…However, the persistence of these compounds in tissues and body fluids of exposed marine organisms varies, depending upon the rates of uptake, metabolism and elimination (Krahn & Stein 1998). Vertebrates, such as fish and marine mammals, quickly metabolize PAHs into more polar forms that are then excreted into urine or secreted into the bile for rapid elimination via the gastroenteric tract (Roubal et al 1977, Krahn et al 1992.…”

Section: Contribution To the Theme Section 'Effects Of The Deepwater mentioning

confidence: 99%

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Determining oil and dispersant exposure in sea turtles from the northern Gulf of Mexico resulting from the Deepwater Horizon oil spill

Ylitalo¹,

Collier²,

Anulacion³

et al. 2017

Endang. Species. Res.

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Section: Contribution To the Theme Section 'Effects Of The Deepwater mentioning

confidence: 99%

“…Sea turtle bile samples (n = 25) were analyzed for fluorescence PAH metabolites using high-performance liquid chromatography with fluorescence detection (HPLC-F) (Krahn et al 1992). Biliary protein content was determined as described in da Silva et al (2006).…”

Section: Biliary Pah Metabolite Analysesmentioning

confidence: 99%

Determining oil and dispersant exposure in sea turtles from the northern Gulf of Mexico resulting from the Deepwater Horizon oil spill

Ylitalo¹,

Collier²,

Anulacion³

et al. 2017

Endang. Species. Res.

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“…The presence of FACs in the bile is an indicator of recent exposure to and metabolism of PACs [25]. Bile was collected from the gall bladder of the sole immediately prior to each mitogen assay to determine the concentration of FACs [25,26].…”

Section: Biliary Fluorescent Aromatic Compoundsmentioning

confidence: 99%

Leukoproliferative response of splenocytes from english sole (Pleuronectes vetulus) exposed to chemical contaminants

Arkoosh

Clemons

Huffman

et al. 1996

Enviro Toxic and Chemistry

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Abstract-The leukoproliferative (LP) response of splenic leukocytes from the marine benthic fish English sole (Pleuronectes vetulus) stimulated with the mitogens lipopolysaccharide (LPS), concanavalin A (Con A), and pokeweed mitogen (PWM) was examined as a biomarker of immunotoxic effects. English sole were exposed to contaminants, either by injection of an organic-solvent extract of a sediment containing polycyclic aromatic compounds (PACs) or placed for up to 5 weeks on a reference sediment containing 0.15 to 1.5% (v/v) of the PAC-contaminated sediment. English sole either injected with the contaminated extract or held on PAC-contaminated sediment had an augmented response to Con A. The LP response to LPS showed no relationship to PAC exposure in laboratoryexposed fish, while PWM showed no consistent relationship to exposure to PACs. In a field study, English sole captured from an urban area in Puget Sound, Washington, USA, contaminated with PACs and other chemical contaminants had a significantly augmented LP response to Con A and PWM in comparison to the LP response in fish from a nonurban reference site. Fish from another nonurban site also had an augmented LP response to Con A, indicating that the elevation of the Con A LP response can also result from factors other than chemical contaminant exposure. In addition, English sole from this site also had an augmented LP response to LPS, whereas fish from urban sites did not exhibit an augmented LP response to LPS. Overall, the results demonstrated that although the LP response in splenic leukocytes of English sole to Con A was linked to contaminant exposure, the LP response to Con A did not exhibit high specificity as an indicator of chemical contaminant exposure. However, the concerted use of Con A, LPS, and PWM allowed for identification of apparent chemical contaminant-induced alterations of the LP response in English sole from an urban area of Puget Sound.

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“…For example, elevated levels of metabolized aromatic hydrocarbons have recently been detected in tissues and fluids from fish samples exposed to the oil spill in Valdez, Alaska [2]. Such elevated PAH levels are of concern because of possible carcinogenic effects and food chain amplification.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence monitoring of polycyclic aromatic hydrocarbon biodegradation and effect of surfactants

Putcha

Domach

1993

Environ. Prog.

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Mass spectrometric analysis for aromatic compounds in bile of fish sampled after the Exxon Valdez oil spill

Cited by 114 publications

References 13 publications

Determining oil and dispersant exposure in sea turtles from the northern Gulf of Mexico resulting from the Deepwater Horizon oil spill

Determining oil and dispersant exposure in sea turtles from the northern Gulf of Mexico resulting from the Deepwater Horizon oil spill

Leukoproliferative response of splenocytes from english sole (Pleuronectes vetulus) exposed to chemical contaminants

Fluorescence monitoring of polycyclic aromatic hydrocarbon biodegradation and effect of surfactants

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